Lipopolysaccharide and a phorbol ester stimulate secretion of tumor necrosis factor-α from alveolar macrophages through action on overlapping subsets of cells

Taylor, Michael J.; Hehnke, K. E.; Clark, Cheryl L.

doi:10.1002/jlb.54.5.384

Cited by 7 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More than 10 yr ago, Murphy and Herscowitz reported functional variability and subpopulations at differing stages of maturity among AM isolated from rabbits (18). From analyzing TNF production by individual porcine AM treated with LPS or phorbol ester, Taylor and colleagues have more recently shown that particulm" smaU subsets of AM respond to distinct stimuli (27), Thus, the variations observed with these AM cell lines most likely reflect the heterogeneity of the original pool of primary cells from which they were derived.…”

Section: Synopsismentioning

confidence: 97%

Characterization of proinflammatory cytokine production and CD14 expression by murine alveolar macrophage cell lines

Ryan

Golenbock

et al. 1997

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

Alveolar macrophages, which play a central role in lung defense, produce cytokines that help orchestrate local inflammatory responses. In sepsis and other pathological conditions, bacterial lipopolysaccharide endotoxin can induce alveolar macrophages (AM) to release proinflammatory cytokines, including tumor necrosis factor-alpha, interleukin-1, and interleukin-6. Studying the mechanisms that control alveolar macrophage cytokine production may lead to better therapies for conditions involving inflammatory lung injury. We and others have noted significant differences between alveolar macrophages and peritoneal macrophages, but large numbers of human or murine alveolar macrophages are rarely available for detailed mechanistic studies. We have obtained three murine alveolar macrophage cell lines (AMJ2C8, AMJ2C11, and AMJ2C20) and have begun to characterize their cytokine responses to proinflammatory stimuli. We measured the effects of endotoxin, interferon gamma, and the combination of the two on production of tumor necrosis factor, interleukin-1 beta, and interleukin-6 in each line. We also studied the expression of the endotoxin receptor CD14 by these cells, and investigated the effect of serum on their endotoxin responsiveness. We show here that all three of the cell lines responded in a manner comparable to that of primary murine alveolar macrophages. Observed variations between these lines may reflect the documented heterogeneity seen in populations of primary alveolar macrophages. These cell lines should expand the repertoire of tools available to investigators studying regulation of murine alveolar macrophage responses.

show abstract

Section: Synopsismentioning

confidence: 97%

Characterization of proinflammatory cytokine production and CD14 expression by murine alveolar macrophage cell lines

Ryan

Golenbock

et al. 1997

In Vitro Cell.Dev.Biol.-Animal

View full text Add to dashboard Cite

show abstract

“…There have been numerous studies of pig macrophage responses to activation that largely recapitulate findings from mouse or human systems. Porcine macrophages from various locations treated with LPS increase production of the major proinflammatory cytokine TNF-␣ [152][153][154] and expression of IL-1␤ [149,155,156], IL-6 [157,158], IL-8 [159,160], and IL-12 [155,161]. Useful microarray platforms are in their comparative infancy in pigs (see below) but have already been used in a study of transcriptional responses within the LNs of pigs infected with salmonella [90] and with monocytes infected with African swine fever virus [162].…”

Section: Inflammatory Cytokine Production In Pigsmentioning

confidence: 99%

The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and disease

Fairbairn

Kapétanovic

Sester

et al. 2011

Journal of Leukocyte Biology

166

144

View full text Add to dashboard Cite

The biology of cells of the mononuclear phagocyte system has been studied extensively in the mouse. Studies of the pig as an experimental model have commonly been consigned to specialist animal science journals. In this review, we consider some of the many ways in which the innate immune systems of humans differ from those of mice, the ways that pigs may address the shortcomings of mice as models for the study of macrophage differentiation and activation in vitro, and the biology of sepsis and other pathologies in the living animal. With the completion of the genome sequence and the characterization of many key regulators and markers, the pig has emerged as a tractable model of human innate immunity and disease that should address the limited, predictive value of rodents in preclinical studies.

show abstract

“…Luteal tissue was then dispersed, and a TNFa-RHPA was performed [20]. Briefly, the luteal cells (0.25 X 10 6 cells/ml) were mixed 1:1 with a 12% suspension of ovine red blood cells to which Protein A (Sigma, St. Louis, MO) had been attached by the chromium conjugation method, and this mixture was placed in incubation chambers (Cunningham chambers).…”

Section: Rhpamentioning

confidence: 99%

Presence and Localization of Tumor Necrosis Factor α in the Corpus Luteum of Nonpregnant and Pregnant Pigs1

Hehnke-Vagnoni

Clark

Taylor

et al. 1995

Biology of Reproduction

View full text Add to dashboard Cite

The objectives of this study were to determine whether tumor necrosis factor alpha (TNF alpha) is present in porcine corpus luteum (CL) and, if so, to ascertain which cells of the CL were associated with TNF alpha. The influence of the reproductive state of the animals on the amount or cellular localization of TNF alpha in the CL was also investigated. Western blot analysis demonstrated the presence of a 34-kDa immunoreactive TNF alpha-like protein in CL from nonpregnant and pregnant pigs. The intensity of TNF alpha immunostaining in bands observed in Western blots did not differ when luteal protein from nonpregnant pigs or from pregnant pigs was analyzed. Positive immunostaining for TNF alpha, determined by immunocytochemistry, was localized within endothelial cells of luteal sections. Positive immunocytochemical staining was recorded in all reproductive states examined except in CL that had undergone functional luteolysis (reduced progesterone concentration). A single-cell assay for TNF alpha secretion (a reverse hemolytic plaque assay) confirmed in vitro secretion of TNF alpha by a cell type in luteal tissue with a size and nuclear morphology similar to an endothelial cell. The presence of TNF alpha in the endothelial cells in functional CL is consistent with a role for TNF alpha in the regulation of vascular development of the CL in swine.

show abstract

Lipopolysaccharide and a phorbol ester stimulate secretion of tumor necrosis factor-α from alveolar macrophages through action on overlapping subsets of cells

Cited by 7 publications

References 17 publications

Characterization of proinflammatory cytokine production and CD14 expression by murine alveolar macrophage cell lines

Characterization of proinflammatory cytokine production and CD14 expression by murine alveolar macrophage cell lines

The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and disease

Presence and Localization of Tumor Necrosis Factor α in the Corpus Luteum of Nonpregnant and Pregnant Pigs1

Contact Info

Product

Resources

About