Lipopolysaccharides at Solid and Liquid Interfaces: Models for Biophysical Studies of the Gram-negative Bacterial Outer Membrane

Paracini, Nicolò; Schneck, Emanuel; Micciulla, Samantha

doi:10.1016/j.cis.2022.102603

Cited by 42 publications

(39 citation statements)

References 169 publications

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“…Also publications on the interface of lipopolysaccharide and lipid A bilayers are not numerous, e.g. ( Snyder et al, 1999 ; Wu et al, 2013 ; Murzyn and Pasenkiewicz-Gierula, 2015 ; Kim et al, 2016 ; Luna et al, 2021 ; Paracini et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Lipid/water interface of galactolipid bilayers in different lyotropic liquid-crystalline phases

Hryc

Szczelina

Markiewicz

et al. 2022

Front. Mol. Biosci.

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In this study, carried out using computational methods, the organisation of the lipid/water interface of bilayers composed of galactolipids with both α-linolenoyl acyl chains is analysed and compared in three different lyotropic liquid-crystalline phases. These systems include the monogalactosyldiglyceride (MGDG) and digalactosyldiglyceride (DGDG) bilayers in the lamellar phase, the MGDG double bilayer during stalk phase formation and the inverse hexagonal MGDG phase. For each system, lipid-water and direct and water-mediated lipid-lipid interactions between the lipids of one bilayer leaflet and those of two apposing leaflets at the onset of new phase (stalk) formation, are identified. A network of interactions between DGDG molecules and its topological properties are derived and compared to those for the MGDG bilayer.

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Section: Introductionmentioning

confidence: 99%

Lipid/water interface of galactolipid bilayers in different lyotropic liquid-crystalline phases

Hryc

Szczelina

Markiewicz

et al. 2022

Front. Mol. Biosci.

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“…The phospholipid outer membrane of Gram-ve bacteria is impermeable to lipophilic substances. Hydrophobic chemicals, which may normally permeate across phospholipid membranes, are one of the key advantages of Gram-ve bacteria's lipopolysaccharides (LPS) layer, allowing them to evade antimicrobials and establishing the basis for their rising antibiotic resistance (Tommasi et al, 2015;Paracini et al, 2022). Such results may explain why Escherichia coli is more resistant to extracts of mangrove than the Gram+ve bacteria evaluated in the current study.…”

Section: Discussionmentioning

confidence: 90%

In vitro antifungal and antibacterial potentials of organic extracts of Avicennia marina collected from Rabigh Lagoon, Red Sea Coasts in Saudi Arabia

et al. 2022

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Mangrove shrub Avicennia marina (Forsk.) Vierh was used to test the antifungal and antibacterial activities of aerial fractions in vitro. Aspergillus sp, Candida sp and Gram positive bacteria have all been found to be sensitive to mangrove extracts, whereas Gram negative bacteria have been found to be resistant to them. Agar disc diffusion and well-cut diffusion were employed to conduct antifungal and antibacterial activities. The MICs (minimum inhibitory concentrations) for each assay have been established. Several extracts from Mangrove reduced fungus growth (diameters fluctuated between 11 and 41 mm). The Ethyl acetate fraction showed particularly strong inhibition of C. tropicalis, C. albicanis, and A. fumigatus. They had 41, 40, and 25 mm-diameter inhibition zones, respectively. Nesoral, a synthetic antifungal medication, showed no significant changes in its MICs compared to different extracts. Enterococcus faecalis and Bacillus subtilis were inhibited by Petroleum Ether extracts at MICs of 0.78 and 0.35 mg/mL, respectively. It is possible that A. marina extracts may be exploited as a viable natural alternative that may be employed in the management of various infections, notably nosocomial bacterial infections, as anti-candidiasis and as anti-aspergillosis agents.

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“…The combination of Langmuir–Blodgett and Langmuir–Schaefer techniques is a method that has been reported to successfully form asymmetric bilayer membranes with high coverage [ 6 , 8 ]. Phospholipids, generally phosphatidylcholine (PC), were deposited as an inner leaflet by the Langmuir–Blodgett and Langmuir–Schaefer deposition techniques, and lipid A or E. coli crude LPS outer leaflets were deposited on an ultra-flat silicon wafer surface [ 8 , 46 ], allowing for the composition of each bilayer leaflet to be precisely controlled [ 47 , 48 ].…”

Section: Biomimetic Bacterial Membrane Systemsmentioning

confidence: 99%

“…A thin layer of peptidoglycan is located in the periplasm, which supports the shape and rigidity of the bacterium. The outer membrane is extremely asymmetric, with a variety of proteins embedded in the membrane that serve as an additional selectively permeable barrier for Gram-negative bacteria [ 5 , 6 ]. Escherichia coli ( E. coli ) is the most well-known of the Gram-negative bacteria species that have been extensively studied [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Solid and Liquid Surface-Supported Bacterial Membrane Mimetics as a Platform for the Functional and Structural Studies of Antimicrobials

Ren

Lyu

et al. 2022

Membranes

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Increasing antibiotic resistance has provoked the urgent need to investigate the interactions of antimicrobials with bacterial membranes. The reasons for emerging antibiotic resistance and innovations in novel therapeutic approaches are highly relevant to the mechanistic interactions between antibiotics and membranes. Due to the dynamic nature, complex compositions, and small sizes of native bacterial membranes, bacterial membrane mimetics have been developed to allow for the in vitro examination of structures, properties, dynamics, and interactions. In this review, three types of model membranes are discussed: monolayers, supported lipid bilayers, and supported asymmetric bilayers; this review highlights their advantages and constraints. From monolayers to asymmetric bilayers, biomimetic bacterial membranes replicate various properties of real bacterial membranes. The typical synthetic methods for fabricating each model membrane are introduced. Depending on the properties of lipids and their biological relevance, various lipid compositions have been used to mimic bacterial membranes. For example, mixtures of phosphatidylethanolamines (PE), phosphatidylglycerols (PG), and cardiolipins (CL) at various molar ratios have been used, approaching actual lipid compositions of Gram-positive bacterial membranes and inner membranes of Gram-negative bacteria. Asymmetric lipid bilayers can be fabricated on solid supports to emulate Gram-negative bacterial outer membranes. To probe the properties of the model bacterial membranes and interactions with antimicrobials, three common characterization techniques, including quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR), and neutron reflectometry (NR) are detailed in this review article. Finally, we provide examples showing that the combination of bacterial membrane models and characterization techniques is capable of providing crucial information in the design of new antimicrobials that combat bacterial resistance.

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Lipopolysaccharides at Solid and Liquid Interfaces: Models for Biophysical Studies of the Gram-negative Bacterial Outer Membrane

Cited by 42 publications

References 169 publications

Lipid/water interface of galactolipid bilayers in different lyotropic liquid-crystalline phases

Lipid/water interface of galactolipid bilayers in different lyotropic liquid-crystalline phases

In vitro antifungal and antibacterial potentials of organic extracts of Avicennia marina collected from Rabigh Lagoon, Red Sea Coasts in Saudi Arabia

Solid and Liquid Surface-Supported Bacterial Membrane Mimetics as a Platform for the Functional and Structural Studies of Antimicrobials

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