Lipopolysaccharides Trigger Two Successive Bursts of Reactive Oxygen Species at Distinct Cellular Locations

Shang-Guan, Keke; Wang, Min; Htwe, Nang Myint Phyu Sin; Li, Ping; Li, Yaoshen; Qi, Fan; Zhang, Dawei; Cao, Min; Kim, Chanhong; Weng, Haiyong; Cen, Haiyan; Black, Ian; Azadi, Parastoo; Carlson, Russell W.; Stacey, Gary; Liang, Yan

doi:10.1104/pp.17.01637

Cited by 63 publications

(43 citation statements)

References 55 publications

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“…In Arabidopsis , ROS production during the immune response is mainly achieved by the NADPH oxidase RBOHD 39 . However, a recent report on LPS perception also suggested that long lasting ROS production could originate from chloroplasts 61 . We found that sustained ROS production triggered by unsaturated SRBs was fully dependent on RBOHD demonstrating that a long lasting oxidative burst could also be generated by the membrane bound NADPH oxidase.…”

Section: Discussionmentioning

confidence: 99%

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Luzuriaga-Loaiza

Schellenberger

Gaetano

et al. 2018

Sci Rep

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Stimulation of plant innate immunity by natural and synthetic elicitors is a promising alternative to conventional pesticides for a more sustainable agriculture. Sugar-based bolaamphiphiles are known for their biocompatibility, biodegradability and low toxicity. In this work, we show that Synthetic Rhamnolipid Bolaforms (SRBs) that have been synthesized by green chemistry trigger Arabidopsis innate immunity. Using structure-function analysis, we demonstrate that SRBs, depending on the acyl chain length, differentially activate early and late immunity-related plant defense responses and provide local increase in resistance to plant pathogenic bacteria. Our biophysical data suggest that SRBs can interact with plant biomimetic plasma membrane and open the possibility of a lipid driven process for plant-triggered immunity by SRBs.

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Section: Discussionmentioning

confidence: 99%

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Luzuriaga-Loaiza

Schellenberger

Gaetano

et al. 2018

Sci Rep

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“…ROS assays were carried out as described previously (Shang‐Guan et al ., ). Briefly, leaf discs (0.2 cm 2 ) were incubated overnight in a 96‐well plate with water, and 200 mM luminol (Sigma‐Aldrich, Saint Louis, MO, USA), 20 mg/mL horseradish peroxidase (Sigma‐Aldrich, Saint Louis, MO, USA), or 100 nM flg22 (GenScript, Nanjing, China) were then added.…”

Section: Methodsmentioning

confidence: 97%

Tomato SlSAP3, a member of the stress‐associated protein family, is a positive regulator of immunity against Pseudomonas syringae pv. tomato DC3000

Liu

Wang

Jiang

et al. 2019

Molecular Plant Pathology

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Summary Tomato stress‐associated proteins (SAPs) belong to A20/AN1 zinc finger protein family, some of which have been shown to play important roles in plant stress responses. However, little is known about the functions and underlying molecular mechanisms of SAPs in plant immune responses. In the present study, we reported the function of tomato SlSAP3 in immunity to Pseudomonas syringae pv. tomato (Pst) DC3000. Silencing of SlSAP3 attenuated while overexpression of SlSAP3 in transgenic tomato increased immunity to Pst DC3000, accompanied with reduced and increased Pst DC3000‐induced expression of SA signalling and defence genes, respectively. Flg22‐induced reactive oxygen species (ROS) burst and expression of PAMP‐triggered immunity (PTI) marker genes SlPTI5 and SlLRR22 were strengthened in SlSAP3‐OE plants but were weakened in SlSAP3‐silenced plants. SlSAP3 interacted with two SlBOBs and the A20 domain in SlSAP3 is critical for the SlSAP3‐SlBOB1 interaction. Silencing of SlBOB1 and co‐silencing of all three SlBOB genes conferred increased resistance to Pst DC3000, accompanied with increased Pst DC3000‐induced expression of SA signalling and defence genes. These data demonstrate that SlSAP3 acts as a positive regulator of immunity against Pst DC3000 in tomato through the SA signalling and that SlSAP3 may exert its function in immunity by interacting with other proteins such as SlBOBs, which act as negative regulators of immunity against Pst DC3000 in tomato.

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“…Plants are also able to sense LPS and activate signaling pathways that ultimately lead to an innate immune response that includes reactive oxygen species (ROS) bursts, callose deposition, nitric oxide production, and transcription of defense-related genes [52][53][54]. The mechanisms underlying the recognition of LPS have only recently started to be understood.…”

Section: Animal and Plant Receptors Recognize Lps And Mount An Immunementioning

confidence: 99%

The Role of Pseudomonas aeruginosa Lipopolysaccharide in Bacterial Pathogenesis and Physiology

2019

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The major constituent of the outer membrane of Gram-negative bacteria is lipopolysaccharide (LPS), which is comprised of lipid A, core oligosaccharide, and O antigen, which is a long polysaccharide chain extending into the extracellular environment. Due to the localization of LPS, it is a key molecule on the bacterial cell wall that is recognized by the host to deploy an immune defence in order to neutralize invading pathogens. However, LPS also promotes bacterial survival in a host environment by protecting the bacteria from these threats. This review explores the relationship between the different LPS glycoforms of the opportunistic pathogen Pseudomonas aeruginosa and the ability of this organism to cause persistent infections, especially in the genetic disease cystic fibrosis. We also discuss the role of LPS in facilitating biofilm formation, antibiotic resistance, and how LPS may be targeted by new antimicrobial therapies.

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Lipopolysaccharides Trigger Two Successive Bursts of Reactive Oxygen Species at Distinct Cellular Locations

Abstract: TITLE 6Lipopolysaccharides trigger two successive bursts of reactive oxygen species at 7 distinct cellular locations 8 AUTHORS

Cited by 63 publications

References 55 publications

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Tomato SlSAP3, a member of the stress‐associated protein family, is a positive regulator of immunity against Pseudomonas syringae pv. tomato DC3000

The Role of Pseudomonas aeruginosa Lipopolysaccharide in Bacterial Pathogenesis and Physiology

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