Lipoprotein lipase activity of adipose tissue and skeletal muscle in insulin-deficient human diabetes

Taskinen, Marja‐Riitta; Nikkilä, Esko A.

doi:10.1007/bf01236268

Cited by 146 publications

(48 citation statements)

References 29 publications

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“…gestational age (37.6 wk) than large-for-date infants (41.2 wk), which might mask the inducing effect of insulin. On the other hand, lipoprotein lipase activity was high in almost all of our infants whereas insulin has been shown to be capable of increasing lipoprotein lipase activity in adult adipose tissue only if the basal lipoprotein lipase activity is low (34,35). Therefore, the similarity of lipoprotein lipase activities in groups 1-111 can be explained by assuming saturation of insulin induction.…”

Section: Resultsmentioning

confidence: 79%

Postheparin Plasma Lipoprotein and Hepatic Lipase Activities in Hyperinsulinemic Infants of Diabetic Mothers and in Large-for-Date Infants at Birth

et al. 1986

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ABSTRACT. To study postheparin plasma lipase activities into the circulation (2). Triglycerides in chylomicrons and very in nonfed newborn infants immediately after birth and to low-density lipoproteins of plasma are hydrolyzed by lipoprotein investigate the possible influence of fetal hyperinsulinemia lipase (3, 4). The lipase is located on the vascular endothelial on lipoprotein lipase activity, we measured lipoprotein and surface of adipose and muscle tissue. It is released into the hepatic lipase activities in 55 macrosomic newborn infants: circulation by heparin. Heparin also releases hepatic lipase from group I consisted of 21 infants born to mothers with insulin-the vascular endothelial surface of the liver. In contrast to lipodependent diabetes. The infants were hyperinsulinemic at protein lipase, the function of hepatic lipase in lipoprotein mebirth and had hypoglycemia and poor lipolysis at the age tabolism has not been fully established (4,5). of 2 h. Group I1 consisted of 18 infants born to mothers On the basis of animal experiments, lipoprotein lipase activity with gestational diabetes. Group I11 consisted of 16 large-of newborn infants has been suggested to be low at birth and to for-date infants born to nondiabetic mothers. The mean increase postnatally (1, 6, 7). If so, infants at birth might be postheparin plasma lipoprotein lipase activities at 2 h of incapable of using fat effectively for energy. Previously, we have age were similar (mean 36 pmol free fatty acids/ml/h; SEM shown that at the age of 1 to 4 days postheparin plasma lipopro-15) in groups 1-111. Lipoprotein lipase activity correlated tein lipase is already at the adult level in breast-milk-fed term negatively with cord-serum triglycerides (range 0.13-1.2 and preterm infants; their hepatic lipase activity is about three mmol/liter) but did not correlate with serum insulin (range times the adult value (8, 9). On the other hand, we found that 5.4-524 pU/ml) or C-peptide (range 0.6-21.0 pglliter). lipoprotein lipase activity increases during the first days of life Hepatic lipase activity was somewhat higher in group I whereas hepatic lipase activity remains unchanged in infants (mean 68 pmol free fatty acids/ml/h; SEM 23) than in receiving fat-containing parenteral nutrition (10). groups I1 and I11 (mean 55 pmol free fatty acids/ml/h; Fetal hyperinsulinemia is associated with macrosomia, hypo-SEM 14). Hemoglobin Alc was the only important factor glycemia, and inhibition of lipolysis in infants born to diabetic explaining the difference in hepatic lipase activities be-mothers (1 1). In adults, insulin is known to induce lipoprotein tween groups. Lipoproteins and apolipoproteins A-I, A-11, lipase (3, 12), and lipoprotein lipase activity of adipose tissue has and B were similar in all three groups. We conclude that been reported to be high in obese adults whose basal serum in large-for-date infants lipoprotein lipase is active at birth insulin level is elevated (13,14). This suggests that fetal hyperwithout exogenous fat induction, and tha...

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Section: Resultsmentioning

confidence: 79%

Postheparin Plasma Lipoprotein and Hepatic Lipase Activities in Hyperinsulinemic Infants of Diabetic Mothers and in Large-for-Date Infants at Birth

et al. 1986

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“…The infants born to insulin-dependent diabetic mothers had a lower gestational age (37.6 wk) than large-for-date infants (41.2 wk), which might mask the inducing effect of insulin. On the other hand, lipoprotein lipase activity was high in almost all of our infants whereas insulin has been shown to be capable of increasing lipoprotein lipase activity in adult adipose tissue only if the basal lipoprotein lipase activity is low (34,35). Therefore, the similarity of lipoprotein lipase activities in groups 1-111 can be explained by assuming saturation of insulin induction.…”

Section: Discussionmentioning

confidence: 79%

Postheparin Plasma Lipoprotein and Hepatic Lipase Activities in Hyperinsulinemic Infants of Diabetic Mothers and in Large-for-Date Infants at Birth

et al. 1986

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ABSTRACT. To study postheparin plasma lipase activities in nonfed newborn infants immediately after birth and to investigate the possible influence of fetal hyperinsulinemia on lipoprotein lipase activity, we measured lipoprotein and hepatic lipase activities in 55 macrosomic newborn infants: group I consisted of 21 infants born to mothers with insulindependent diabetes. The infants were hyperinsulinemic at birth and had hypoglycemia and poor lipolysis at the age of 2 h. Group I1 consisted of 18 infants born to mothers with gestational diabetes. Group I11 consisted of 16 largefor-date infants born to nondiabetic mothers. The mean postheparin plasma lipoprotein lipase activities at 2 h of age were similar (mean 36 pmol free fatty acids/ml/h; SEM 15) in groups 1-111. Lipoprotein lipase activity correlated negatively with cord-serum triglycerides (range 0.13-1.2 mmol/liter) but did not correlate with serum insulin (range 5.4-524 pU/ml) or C-peptide (range 0.6-21.0 pgtliter). Hepatic lipase activity was somewhat higher in group I (mean 68 pmol free fatty acids/ml/h; SEM 23) than in groups I1 and I11 (mean 55 pmol free fatty acids/ml/h; SEM 14). Hemoglobin Alc was the only important factor explaining the difference in hepatic lipase activities between groups. Lipoproteins and apolipoproteins A-I, A-11, and B were similar in all three groups. We conclude that in large-for-date infants lipoprotein lipase is active at birth without exogenous fat induction, and that these infants are capable of hydrolyzing fat, their main source of energy, immediately after birth. In addition, we conclude that postheparin plasma lipoprotein lipase activity is not affected by fetal hyperinsulinemia. (Pediafr Res 20: 527-531,1986) Abbreviations FFA, free fatty acids Hb, hemoglobin Soon after birth fat becomes the major metabolic fuel (1): breast-milk fat is readily absorbed through the bowel wall as chylomicrons and liver produces endogenous lipoproteins into

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“…The mechanism by which basal LPL regulates its response to insulin remains a mystery. Its physiological r61e could be to prevent excessive storage of fat in adipose tissue and, on the other hand, to promote storage of fat after a period of lipolysis such as may follow insulin deficiency in diabetics [1] or a reduction of insulin secretion during fasting [3].…”

Section: Discussionmentioning

confidence: 99%

“…Insulin deficiency and insulin resistance in diabetic subjects are associated with subnormal levels of LPL which are restored with insulin treatment [1,2]. In healthy subjects, adipose tissue LPL decreases during fasting [3,4] and increases with carbohydrate feeding [5][6][7].…”

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confidence: 99%

Response of adipose tissue lipoprotein lipase activity and serum lipoproteins to acute hyperinsulinaemia in man

et al. 1984

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Summary. In order to assess the short-term effects of hyperinsulinaemia and hyperglycaemia on adipose tissue lipoprotein lipase activity and on serum lipoproteins, we measured these variables in ten normal subjects during euglycaemic and hyperglycaemic hyperinsulinaemic clamps. The mean steadystate plasma glucose and insulin concentrations, respectively, were 4.7 mmol/1 and 101 mU/1 during euglycaemic moderateinsulin clamp, 4.9 mmol/1 and 565 mU/1 during euglycaemic high-insulin clamp, and 8.8 mmol/1 and 148 mU/l during hyperglycaemic clamp. Saline infusion was used as control. The adipose tissue lipoprotein lipase activity rose significantly over 5 h during high-insulin clamp (p< 0.01) and during hyperglycaemic clamp (p< 0.05), but did not change during the moderate-insulin clamp. The magnitude of change of lipoprotein lipase activity from baseline (either rise or fall) was inversely related to the preclamp activity during euglycaemic moderate-insulin clamp (r=-0.67), during hyperglycaemic clamp (r= -0.68) and during infusion of saline (r= -0.75, p<0.05). Total serum triglyceride concentration decreased significantly during all clamp studies compared with the control experiment. This change was mainly accounted for by a decrease of VLDL triglyceride. The LDL cholesterol level fell by an average of 5% (p< 0.05) during the high-insulin clamp and by 10% (p< 0.05) during the hyperglycaemic clamp. The HDL cholesterol level did not change significantly. It is concluded that adipose tissue lipoprotein lipase activity in man is increased by physiological insulin levels during hyperglycaemia and also by supraphysiological insulin levels during euglycaemia, but is not influenced by physiological hyperinsulinaemia without hyperglycaemia. Low basal lipoprotein lipase activity is more sensitive to insulin-glucose stimulation than primarily high lipoprotein lipase activity. Acute hyperinsulinaemia decreases VLDL triglyceride and LDL cholesterol concentrations.

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Lipoprotein lipase activity of adipose tissue and skeletal muscle in insulin-deficient human diabetes

Cited by 146 publications

References 29 publications

Postheparin Plasma Lipoprotein and Hepatic Lipase Activities in Hyperinsulinemic Infants of Diabetic Mothers and in Large-for-Date Infants at Birth

Postheparin Plasma Lipoprotein and Hepatic Lipase Activities in Hyperinsulinemic Infants of Diabetic Mothers and in Large-for-Date Infants at Birth

Postheparin Plasma Lipoprotein and Hepatic Lipase Activities in Hyperinsulinemic Infants of Diabetic Mothers and in Large-for-Date Infants at Birth

Response of adipose tissue lipoprotein lipase activity and serum lipoproteins to acute hyperinsulinaemia in man

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