2014
DOI: 10.1128/jvi.02297-13
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Liposome-Antigen-Nucleic Acid Complexes Protect Mice from Lethal Challenge with Western and Eastern Equine Encephalitis Viruses

Abstract: Alphaviruses are mosquito-borne viruses that cause significant disease in animals and humans. Western equine encephalitis virus (WEEV) and eastern equine encephalitis virus (EEEV), two New World alphaviruses, can cause fatal encephalitis, and EEEV is a select agent of concern in biodefense. However, we have no antiviral therapies against alphaviral disease, and current vaccine strategies target only a single alphavirus species. In an effort to develop new tools for a broader response to outbreaks, we designed … Show more

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Cited by 17 publications
(28 citation statements)
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“…Immunization with the CLNC/WEEV/E1ecto mixture provided full protection after challenge. Passive serum transfer from immunized to naïve mice conferred protection to challenge, indicating that antibody is sufficient for protection [Phillips et al 2014]. Liposomes containing different cationic compounds and neutral DPPC were loaded with influenza HA by adsorption.…”
Section: Other Cationic Lipid Complexesmentioning
confidence: 99%
“…Immunization with the CLNC/WEEV/E1ecto mixture provided full protection after challenge. Passive serum transfer from immunized to naïve mice conferred protection to challenge, indicating that antibody is sufficient for protection [Phillips et al 2014]. Liposomes containing different cationic compounds and neutral DPPC were loaded with influenza HA by adsorption.…”
Section: Other Cationic Lipid Complexesmentioning
confidence: 99%
“…This immunization provided 100 % protection in mice challenged with WEEV subcutaneously, intranasally, or via mosquito. Moreover, 90 % of the mice survived EEEV challenge, demonstrating strong cross-protection of the WEEV immunization and the potential to develop a pan-encephalitic alphavirus vaccine [41]. [28].…”
Section: Vaccines Under Development For Zoonotic Alphaviruses Encephamentioning
confidence: 98%
“…As current vaccine strategies target only a single alphavirus species (VEEV), Phillips et al [41] designed an alphavirus vaccine comprised of cationic lipid nucleic acid complexes and the ectodomain of the WEEV E1 protein. This immunization provided 100 % protection in mice challenged with WEEV subcutaneously, intranasally, or via mosquito.…”
Section: Vaccines Under Development For Zoonotic Alphaviruses Encephamentioning
confidence: 99%
“…Passage history for virus strains used has been previously published (13,14). Detailed descriptions of the molecular cloning methods used to construct recombinant WEEV.McM reporter viruses are provided below and have been previously published (7).…”
Section: Virusesmentioning
confidence: 99%