Liposomes and immunoassays

Rongen, H.A.H.; Bult, A.; Bennekom, W.P. van

doi:10.1016/s0022-1759(97)00041-0

Cited by 163 publications

(90 citation statements)

References 139 publications

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“…Free uricase (250 µg) with 0.5 mL of blank lipid vesicles (used as an immunological adjuvant) 23 or UOXLVs (containing the equivalent dose of uricase) were multipoint injected into the rats subcutaneously. 24 The rats were boosted every 2 weeks for 6 weeks with the corresponding antigen. Blood was collected prior to the first injection and on day 7 after the last injection.…”

Section: Immunological Testmentioning

confidence: 99%

Improved biological properties and hypouricemic effects of uricase from Candida utilis loaded in novel alkaline enzymosomes

Tan¹,

Zhang²,

Wang³

et al. 2012

IJN

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Objective: Previous studies on various enzymosomes (functional lipid vesicles encapsulating an enzyme) have been mostly carried out in vitro and have focused on preserving catalytic activity and improving the stability of the enzyme. Until now, few studies have focused on their in vivo fate. Similarly, although we have previously reported the increased in vitro uricolytic activity (about 2.2 times higher than that of free uricase, or three times higher than that of PEGylated uricase, Puricase ® , under physiological pH and temperature) and improved stability of the novel alkaline enzymosomes (functional lipid vesicles encapsulating uricase from Candida utilis: uricase-containing lipid vesicles, UOXLVs), it is still necessary to study the biological properties and hypouricemic effects of UOXLVs in vivo. Methods:The enzyme kinetics, pharmacokinetics, pharmacodynamics, immunogenicity, and preliminary safety of UOXLVs were evaluated. Results: The Michaelis constant (K m ) value of the UOXLVs was slightly lower than that of the free enzyme. The enzyme release from the UOXLVs lasted over 12 hours and their circulation half-life was about sevenfold longer than that of the free uricase. Meanwhile, the UOXLVs had a 22-fold increase in the area under the curve compared with the free uricase. Furthermore, it took less than 3 hours for the UOXLVs to lower the plasma uric acid concentration from a high to a normal level, compared with 6 hours for the free uricase. In addition, the UOXLVs had much less immunogenicity than free uricase and were well tolerated by all animals throughout the observation period. Conclusion:The UOXLVs markedly improved the biological properties and enhanced the hypouricemic effects of uricase in vivo.

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Section: Immunological Testmentioning

confidence: 99%

Improved biological properties and hypouricemic effects of uricase from Candida utilis loaded in novel alkaline enzymosomes

Tan¹,

Zhang²,

Wang³

et al. 2012

IJN

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“…Antibodies were thiolated and attached to the DSPE-PEG2000-maleimide moiety of the nanosomes and isolated from the free antibody by gel filtration. 21,[30][31][32] For the control nanosomes, the antibody was not thiolated, but the antibody was mixed with the nanosomes. Equivalent size distribution, NIF labeling, phospholipid content, and antibody labeling among the different nanosome preparations were confirmed by transmission electron microscopy (TEM), fluorometry, and dynamic light scattering (DLS).…”

Section: Nanosome Chemistrymentioning

confidence: 99%

Noninvasive visualization of early osteoarthritic cartilage using targeted nanosomes in a destabilization of the medial meniscus mouse model

Cho¹,

Kim²,

Park³

et al. 2018

IJN

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Background Early stage osteoarthritis (OA) is clinically asymptomatic due to the avascular and the aneural nature of the cartilage tissue. Nevertheless, early detection of cartilage tissue is critical in order to impede the progression of OA. Hence, in order to develop effective preventive therapy for OA, diagnosis in the early stages is necessary. Methods To achieve this goal, we have developed targeted, fluorescent nanosomes conjugated with monoclonal anti-type II collagen antibodies (MabCII) for diagnosis of early OA. The MabCII-coated nanosomes (targeted-nanosomes) bind to the damaged cartilage explants in vitro and in vivo in an OA mouse model that mimics early stage OA. The OA mouse model was induced by destabilization of the medial meniscus (DMM) in 9–10 weeks old C57Bl/6 mice. Results The targeted-nanosomes enhanced the binding specificity to the cartilage tissue according to the severity of damage. Conclusion We show that MabCII-nanosomes can precisely detect early stage OA in the DMM mouse model. Thus, MabCII-nanosomes have the potential to be used as a non-invasive method for diagnosing the early osteoarthritic lesions.

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“…특히, 리포좀의 외부표면에 항체를 결합 시켜 만든 면역리포좀은 항체에 대한 특정 항원에만 작용 하므로 항원-항체 반응을 이용한 다양한 면역분석에 이용 될 수 있다. 면역리포좀을 이용한 면역분석법의 개발에 관 한 연구는 농약, 약물, 자연독소 등 광범위한 물질을 대상 으로 보고되고 있다 (Rongen et al, 1997). 면역분석은 수 행하기가 편리하고 신속하게 결과를 얻을 수 있다는 장점 과 더불어 간편한 키트의 형태로 제품화가 가능하므로 각 종 정성, 정량 분석에 범용적으로 쓰이고 있다.…”

Section: 서 론unclassified

Analysis of Salmonella Species from Eggs Using Immunoliposomes and Comparison with a Commercial Test Kit

Shin¹,

Kim²,

Lee³

et al. 2009

Korean Journal for Food Science of Animal Resources

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To suggest an improved diagnostic method for Salmonella spp., analyses were conducted with immunoliposomes and compared with the results from a commercial test kit. One sample out of 36 samples of eggshell was Salmonella-positive via immunoliposomes. In the case of the use of the commercial test kit, six samples out of 36 samples were Salmonellapositive. These Salmonella-positive samples were subjected to biochemical identification tests that confirmed that they were Salmonella-negative. As for the egg content samples, they were Salmonella-negative in both analyses with immunoliposomes and the commercial test kit. The Salmonella analysis with immunoliposomes reduced detection time, by 24 h compared to the commercial test kit. Bacteria, including Acinetobacter baumanni, Chryseomonas luteola, Enterobacter cloacae, Escherichia coli, Escherichia hermannii, Klebsiella pneumonia, Pantoea spp., and Pasteurella pneumotropica, were isolated from the eggshells. Other than Acinetobacter baumanni and Pasteurella pneumotropica most of the isolates were known to frequently appear during egg production processing.

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Liposomes and immunoassays

Cited by 163 publications

References 139 publications

Improved biological properties and hypouricemic effects of uricase from Candida utilis loaded in novel alkaline enzymosomes

Improved biological properties and hypouricemic effects of uricase from Candida utilis loaded in novel alkaline enzymosomes

Noninvasive visualization of early osteoarthritic cartilage using targeted nanosomes in a destabilization of the medial meniscus mouse model

Analysis of Salmonella Species from Eggs Using Immunoliposomes and Comparison with a Commercial Test Kit

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