Liposomes in chromatography

Cserháti, Tibor; Szőgyi, M.

doi:10.1002/bmc.1444

Cited by 14 publications

(9 citation statements)

References 55 publications

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“…Beside nanoparticles, carbon‐based nanomaterials such as nanotubes, fullerenes, graphene which exhibit very good optical, electrochemical, and adsorptive properties are subject of constant investigation . Other nanomaterials which cannot be neglected nor considered less important are liposomes and dendrimers .…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis of quantum dots: Minireview

et al. 2014

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It has been already three decades, since the fluorescent nanocrystals called quantum dots (QDs) appeared and attracted attention of a broad scientific community. Their excellent not only optical but also electronic properties predetermined QDs for utilization in a variety of areas. Besides lasers, solar cells, and/or computers, QDs have established themselves in the field of (bio)chemical labeling as well as medical imaging. However, due to the numerous application possibilities of QDs, there are high demands on their properties that need to be precisely controlled and characterized. CE with its versatile modes and possibilities of detection was found to be an effective tool not only for characterization of QDs size and/or surface properties but also for monitoring of their interactions with other molecules of interest. In this minireview, we are giving short insight in analysis of QDs by CE, and summarizing the advantages of this method for QDs characterization.

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Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis of quantum dots: Minireview

et al. 2014

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“…An especially attractive feature of the techniques is the possibility to on‐line couple surface plasmon resonance to MS for quantification and identification of substrates . Lipidomics in general is a growing field, and much research has been carried out on the analysis of liposomes by various techniques and on the use of liposomes in analyses, as well as on analyte–lipid membrane interactions; see [] and references therein.…”

Section: Introductionmentioning

confidence: 99%

Study on capillaries covalently bound with phospholipid vesicles for open‐tubular CEC and application to on‐line open‐tubular CEC‐MS

2013

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Phospholipid vesicles were covalently attached to iminoaldehyde-coated fused silica capillaries and applied to the separation of model steroids by open-tubular CEC (OT-CEC). The effects of reducing the formed Schiff's base with sodium borohydride and of the liposome composition on the stability of the coating were investigated. In addition, the studies were focused on the optimization of running conditions (pH values and composition of BGE solution) when CEC, using capillaries covalently bound with liposome dispersions, was coupled to MS. The effect of cholesterol in the liposome dispersion on the binding of model analytes was studied, using liposome dispersions comprising 80/20 mol% zwitterionic 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (POPC) and the negatively charged phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) and 40/40/20 mol% POPC/POPS/cholesterol. Cholesterol in liposomes (greatly) enhanced the stability of the capillaries by making the coatings more rigid, resulting in lower retention factors for all the studied model steroids. Although most of the studies were carried out by open tubular CEC-UV Vis, the applicability of the capillaries to on-line CEC-MS was demonstrated as well. On-line CEC-MS studies on model steroids proved the suitability of coated capillaries for analyte-lipid membrane interaction studies, and especially for such analytes that are difficult to detect by conventional on-line UV Vis.

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“…Several studies on drug partition behavior have for example employed liposomes passively immobilized to chromatographic gel filtration media (2,3). More recently, chromatographic systems based on covalent coupling of liposomes to silica gels has also been reported (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Immobilized lipodisks as model membranes in high-throughput HPLC-MS analysis

et al. 2013

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This is an accepted version of a paper published in Analytical and Bioanalytical Chemistry. This paper has been peer-reviewed but does not include the final publisher proof-corrections or journal pagination.Citation for the published paper: Meiby, E., Morin Zetterberg, M., Ohlson, S., Agmo Hernańdez, V., Edwards, K. (2013 AbstractLipodisks, also referred to as polyethylene glycol (PEG)-stabilized bilayer disks, have previously been demonstrated to hold great potential as model membranes in drug partition studies. In this study, an HPLC-MS system with stably immobilized lipodisks is presented. Functionalized lipodisks were immobilized on two different HPLC support materials either covalently by reductive amination or by streptavidin-biotin binding. An analytical HPLC column with immobilized lipodisks was evaluated by analysis of mixtures containing15 different drug compounds. The efficiency, reproducibility and stability of the system were found to be excellent. In situ incorporation of cyclooxygenase-1 (COX-1) in immobilized lipodisks on a column was also achieved. Specific binding of COX-1 to the immobilized lipodisks was validated by interaction studies with QCM-D. These results taken together open up for the potential to study ligand interactions with membrane proteins by weak affinity chromatography (WAC).

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Liposomes in chromatography

Cited by 14 publications

References 55 publications

Capillary electrophoresis of quantum dots: Minireview

Capillary electrophoresis of quantum dots: Minireview

Study on capillaries covalently bound with phospholipid vesicles for open‐tubular CEC and application to on‐line open‐tubular CEC‐MS

Immobilized lipodisks as model membranes in high-throughput HPLC-MS analysis

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