Liquid Biopsy: An Emerging Diagnostic, Prognostic, and Predictive Tool in Gastric Cancer

Han, Hye Sook; Lee, Keun-Wook

doi:10.5230/jgc.2024.24.e5

Cited by 8 publications

(2 citation statements)

References 144 publications

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“…Accordingly, it infers that cancer cells gain growth superiority over their regular counterparts by switching metabolic energy patterns to anaerobic glycolysis and possibly fumarate respiration instead of securing more ATP. These studies, including ours, collectively support that understanding the metabolic risk of many malignancies, including esophageal cancer, cancer of the gastroesophageal junction, and gastric cancer, could improve the development of precise oncological and patient-tailored management of UGC (Han and Lee 2024 ). Overall, these data justify the evaluation of existing models of amino acid, lipid, carbohydrates and nucleic acid metabolism and their role in tumour growth, metastasis, and immunosurveillance.…”

Section: Discussionsupporting

confidence: 61%

Metabolomic profiling of upper GI malignancies in blood and tissue: a systematic review and meta-analysis

Balonov,

Mattis,

Jarmusch

et al. 2024

J Cancer Res Clin Oncol

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Objective To conduct a systematic review and meta-analysis of case–control and cohort human studies evaluating metabolite markers identified using high-throughput metabolomics techniques on esophageal cancer (EC), cancer of the gastroesophageal junction (GEJ), and gastric cancer (GC) in blood and tissue. Background Upper gastrointestinal cancers (UGC), predominantly EC, GEJ, and GC, are malignant tumour types with high morbidity and mortality rates. Numerous studies have focused on metabolomic profiling of UGC in recent years. In this systematic review and meta-analysis, we have provided a collective summary of previous findings on metabolites and metabolomic profiling associated with EC, GEJ and GC. Methods Following the PRISMA procedure, a systematic search of four databases (Embase, PubMed, MEDLINE, and Web of Science) for molecular epidemiologic studies on the metabolomic profiles of EC, GEJ and GC was conducted and registered at PROSPERO (CRD42023486631). The Newcastle–Ottawa Scale (NOS) was used to benchmark the risk of bias for case-controlled and cohort studies. QUADOMICS, an adaptation of the QUADAS-2 (Quality Assessment of Diagnostic Accuracy) tool, was used to rate diagnostic accuracy studies. Original articles comparing metabolite patterns between patients with and without UGC were included. Two investigators independently completed title and abstract screening, data extraction, and quality evaluation. Meta-analysis was conducted whenever possible. We used a random effects model to investigate the association between metabolite levels and UGC. Results A total of 66 original studies involving 7267 patients that met the required criteria were included for review. 169 metabolites were differentially distributed in patients with UGC compared to healthy patients among 44 GC, 9 GEJ, and 25 EC studies including metabolites involved in glycolysis, anaerobic respiration, tricarboxylic acid cycle, and lipid metabolism. Phosphatidylcholines, eicosanoids, and adenosine triphosphate were among the most frequently reported lipids and metabolites of cellular respiration, while BCAA, lysine, and asparagine were among the most commonly reported amino acids. Previously identified lipid metabolites included saturated and unsaturated free fatty acids and ketones. However, the key findings across studies have been inconsistent, possibly due to limited sample sizes and the majority being hospital-based case–control analyses lacking an independent replication group. Conclusion Thus far, metabolomic studies have provided new opportunities for screening, etiological factors, and biomarkers for UGC, supporting the potential of applying metabolomic profiling in early cancer diagnosis. According to the results of our meta-analysis especially BCAA and TMAO as well as certain phosphatidylcholines should be implicated into the diagnostic procedure of patients with UGC. We envision that metabolomics will significantly enhance our understanding of the carcinogenesis and progression process of UGC and may eventually facilitate precise oncological and patient-tailored management of UGC.

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Section: Discussionsupporting

confidence: 61%

Metabolomic profiling of upper GI malignancies in blood and tissue: a systematic review and meta-analysis

Balonov,

Mattis,

Jarmusch

et al. 2024

J Cancer Res Clin Oncol

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“…This approach also holds potential for early screening and monitoring of minimal residual disease [6]. Liquid biopsy captures tumor fragments or circulating tumor contents from body fluids such as blood [7], cerebrospinal fluid [8], ascites [9], saliva [10], bronchoalveolar lavage [11], and urine [12]. This allows for repeated assessments during diagnosis, treatment, and follow-up periods with less invasiveness and minimized complications [13,14].…”

Section: Introductionmentioning

confidence: 99%

Extracellular Vesicles for Childhood Cancer Liquid Biopsy

Singhto,

Pongphitcha,

Jinawath

et al. 2024

Cancers

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Liquid biopsy involves the utilization of minimally invasive or noninvasive techniques to detect biomarkers in biofluids for disease diagnosis, monitoring, or guiding treatments. This approach is promising for the early diagnosis of childhood cancer, especially for brain tumors, where tissue biopsies are more challenging and cause late detection. Extracellular vesicles offer several characteristics that make them ideal resources for childhood cancer liquid biopsy. Extracellular vesicles are nanosized particles, primarily secreted by all cell types into body fluids such as blood and urine, and contain molecular cargos, i.e., lipids, proteins, and nucleic acids of original cells. Notably, the lipid bilayer-enclosed structure of extracellular vesicles protects their cargos from enzymatic degradation in the extracellular milieu. Proteins and nucleic acids of extracellular vesicles represent genetic alterations and molecular profiles of childhood cancer, thus serving as promising resources for precision medicine in cancer diagnosis, treatment monitoring, and prognosis prediction. This review evaluates the recent progress of extracellular vesicles as a liquid biopsy platform for various types of childhood cancer, discusses the mechanistic roles of molecular cargos in carcinogenesis and metastasis, and provides perspectives on extracellular vesicle-guided therapeutic intervention. Extracellular vesicle-based liquid biopsy for childhood cancer may ultimately contribute to improving patient outcomes.

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Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients

Čeri,

Somborac-Bačura,

Fabijanec

et al. 2024

Sci Rep

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Liquid biopsy has an underexplored diagnostic potential in colorectal cancer (CRC). Sufficient quantity and quality of its elements (circulating cell-free DNA (ccfDNA), exosomes and exosomal RNA) are essential for accurate results. The present study aims to establish the optimal protocol for handling liquid biopsy samples. Samples were obtained by collecting peripheral blood from colorectal adenoma patients in CellSave tubes. Plasma was separated within six hours using differential centrifugation and aliquots stored at – 20/– 80 °C until further processing. Three methods for isolation of ccfDNA, and two combinations of kits for isolation of exosomes and exosomal RNA were tested. The quality and quantity of ccfDNA isolates were evaluated. Exosomes were characterised by determining size, concentration, and total and specific protein content. Expression of chosen microRNAs, miR-19a-3p and miR-92-3p, which have been implicated in CRC progression, were determined. The vacuum-column-based kit showed the highest quantities of isolated ccfDNA (P-value < 0.001). Kits for exosome isolation significantly differed in size (P-value = 0.016), concentration (P-value = 0.016) and protein content (P-value = 0.016). There was no significant difference in expressions of miR-19a-3p (P-value = 0.219) and miR-92a-3p (P-value = 0.094) between the two isolation kits. The new, adapted protocol described, enables simultaneous analysis of multiple elements when investigating potential biomarkers of CRC.

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Liquid Biopsy: An Emerging Diagnostic, Prognostic, and Predictive Tool in Gastric Cancer

Cited by 8 publications

References 144 publications

Metabolomic profiling of upper GI malignancies in blood and tissue: a systematic review and meta-analysis

Metabolomic profiling of upper GI malignancies in blood and tissue: a systematic review and meta-analysis

Extracellular Vesicles for Childhood Cancer Liquid Biopsy

Establishment of liquid biopsy procedure for the analysis of circulating cell free DNA, exosomes, RNA and proteins in colorectal cancer and adenoma patients

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