Liquid Chromatographic Analysis and Separation of Polypeptide Components from Honey Bee Venoms

Szókán, Gy.; Horváth, Judit; Almás, M.; Saftics, Gy.; Palócz, A.

doi:10.1080/10826079408013516

Cited by 24 publications

(8 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More sensitivity and better separation efficiency were involved than for other methods (Tab. 2).The sample concentration, limits of detection and quantification, values, and retention times (t R ) for melittin were less than those previously reported (Szokan et al, 1994;Pacáková et al, 1995;Rybak-Chmielewska and Szczęsna, 2004;Kokot and Matysiak, 2009). There was a significant difference of melittin in samples.…”

Section: Analysis Of Honeybee Venom and Its Medicinal Productsmentioning

confidence: 66%

“…The amount of melittin in the bee venom samples was 21.9, 23.7, 28.2, 31.8, 41.7, 46.4, 51.3, and 66.4%. The amount of melittin in Table 1 Szokan et al, (1994) 5000 μg/mL --31.2 Pacáková et al, (1995) 500 μg/mL 3 -18.1 Rybak -Chmielewska and Szczęsna (2004) 5000 μg/mL 40 -25.7 Kokot and Matysiak (2009) 300 μg /mL 4.8 14.4 16.4…”

Section: Resultsmentioning

confidence: 99%

“…The amount of melittin in the honeybee venom samples collected during the summer season ranged from 21.9 to 66.4% with a mean value of 38.9%. Szokan et al (1994) found the melittin content in bee venom samples to be 50-60%. In another study, the amount of melittin was reported to be 61 to 71% (Rybak-Chmielewska and Szczęsna, 2004).…”

Section: Analysis Of Honeybee Venom and Its Medicinal Productsmentioning

confidence: 99%

“…This peptide was named melittin by Ernst Habermann (Gauldie et al, 1976). Several analytical techniques have been reported for determinating biological compounds of honeybees, such as HPLC coupled with detections of ultraviolet, diode array and mass spectrometry (Rader et al, 1987;Szokan et al, 1994;Pacáková et al, 1995;Ziyavitdinov et al, 1995;Rybak-Chmielewska and Szczęsna 2004;Kokot and Matysiak, 2009;Zhou et al, 2010), and capillary electrophoresis (CE) (Pacáková et al, 1995;Pacáková and Stulik, 2000;Matysiak et al, 2011). In pharmaceutical industry, the RP-HPLC method is one of the most useful techniques for quality control of drugs formulations.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Qualitative and quantitative evaluation of melittin in honeybee venom and drug products containing honeybee venom

Haghi¹,

Hatami²,

Mehran³

2013

Journal of Apicultural Science

View full text Add to dashboard Cite

a b s t r a c t a reverse-phase high-performance liquid chromatographic (rp-hplc) method was developed and validated for the analysis of honeybee venom samples and drug products containing honeybee venom. the validation parameters were linearity, sensitivity, precision, and recovery. melittin is the main component of honeybee venom was extracted with pure water, and then evaluated by rp-hplc with a photodiode array (pda) detector. separation of the samples was achieved on a europa protein c 18 column with linear gradient elution of acetonitrile and 0.4% phosphoric acid at 25°c. there was a flow rate of 1 ml/min. detection was set at 220 nm. limits of detection (lod) and quantification (loQ) for melittin were 1.1 and 3.2 µg/ml, respectively. the amount of melittin in honeybee venom samples ranged from 21.9 to 66.4 %.

show abstract

Section: Analysis Of Honeybee Venom and Its Medicinal Productsmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Section: Analysis Of Honeybee Venom and Its Medicinal Productsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Qualitative and quantitative evaluation of melittin in honeybee venom and drug products containing honeybee venom

Haghi¹,

Hatami²,

Mehran³

2013

Journal of Apicultural Science

View full text Add to dashboard Cite

show abstract

“…Reversed-phase (RP) HPLC is an important method of evaluating BV tablets 19) . Therefore, we analyzed two kinds of BV pharmacopuncture (BV-I and II), three kinds of SPBV pharmacopuncture (SPBV-I, II, and III), and apitoxin using HPLC.…”

Section: Discussionmentioning

confidence: 99%

Major Components of Clinically used Bee Venom Pharmacopuncture^※

Roh

2017

The Acupuncture

View full text Add to dashboard Cite

[Abstract]Objectives : Thepurposeofthisstudywastoanalyzethecomponentsoftheclinicallyusedbee venom(BV)pharmacopuncture. Methods : Twokindsofbeevenompharmacopuncture(BV-IandII),threekindsofseparatepurificationBV(SPBV)pharmacopuncture(SPBV-I,II,andIII),andapitoxinwereinvestigatedin thisstudy.Weperformedacomponentanalysisofmelittin,apamin,andphospholipaseA 2 usinghigh-performanceliquidchromatography(HPLC). Results : 1.BV-Icontainedapproximately40%moremelittinthanBV-IIdid.2.InthethreeseparatepurificationBVpharmacopuncture,SPBV-I,SPBV-II,andSPBV-III, phospholipaseA 2 contentdecreasedremarkably. 3.ThemelittincontentinSPBV-Iincreasedby5%comparedtothatinBV-I. 4.TheamountofmelittininapitoxinwassimilartothatinSPBV-I.

show abstract

New CZE-DAD method for honeybee venom analysis and standardization of the product

et al. 2011

View full text Add to dashboard Cite

The aim of this study was to develop a new precise and accurate CZE-DAD method for honeybee venom analysis using cytochrome c as an internal standard. The 64.5 cm total length, 56 cm effective length, 75 μm ID, and 360 μm OD uncoated fused-silica capillary was used. The samples were injected into the capillary under a 50-mbar pressure for 7 s. There were 15 kV of electric field across the capillary applied. The current intensity was 26 μA. The separation was carried out at 25 °C. The analysis was run with the normal electrode polarity. The following steps and parameters were taken into account for the validation of the developed method: selectivity, precision, accuracy, linearity, limit of detection and limit of quantitation. All steps of the validation procedure proved that the developed analytical procedure was suitable for its intended purpose. Possibly this was the first study in which several honeybee venom components were separated and five of them were identified by capillary zone electrophoresis. In addition, the developed method was applied for quantitative analysis of 38 honeybee venom samples. The content (relative to the dry venom mass) of analyzed peptides in honeybee venom samples collected in 2002–2007 was as follows: apamine from 0.93% to 4.34% (mean, 2.85 ± 0.79%); mast cell degranulating peptide (MCDP) from 1.46% to 4.37% (mean, 2.82 ± 0.64%); phospholipase A2 from 7.41% to 20.25% (mean, 12.95 ± 3.09%); melittin from 25.40% to 60.27%, (mean, 45.91 ± 9.78%). The results were compared with the experimental data obtained for the same venom samples analyzed earlier by the HPLC method. It was stated that HPCE and HPLC data did not differ significantly and that the HPCE method was the alternative for the HPLC method. Moreover, using the results obtained principal component analysis (PCA) was applied to clarify the general distribution patterns or similarities of four major honeybee venom constituents collected from two different bee strains in various months and years. PCA has shown that the strain of bee appears to be the only criteria for bee venom sample classification. Strong correlations between apamine, MCDP, phospholipase A2, and melittin were confirmed. These correlations have to be taken into account in the honeybee venom standardization. The developed method due to its simplicity can be easily automated and incorporated into routine operations both in the bee venom identification, quality control, and standardization of the product.Electronic supplementary materialThe online version of this article (doi:10.1007/s00216-010-4627-2) contains supplementary material, which is available to authorized users.

show abstract

Liquid Chromatographic Analysis and Separation of Polypeptide Components from Honey Bee Venoms

Cited by 24 publications

References 18 publications

Qualitative and quantitative evaluation of melittin in honeybee venom and drug products containing honeybee venom

Qualitative and quantitative evaluation of melittin in honeybee venom and drug products containing honeybee venom

Major Components of Clinically used Bee Venom Pharmacopuncture^※

New CZE-DAD method for honeybee venom analysis and standardization of the product

Contact Info

Product

Resources

About

Liquid Chromatographic Analysis and Separation of Polypeptide Components from Honey Bee Venoms

Cited by 24 publications

References 18 publications

Qualitative and quantitative evaluation of melittin in honeybee venom and drug products containing honeybee venom

Qualitative and quantitative evaluation of melittin in honeybee venom and drug products containing honeybee venom

Major Components of Clinically used Bee Venom Pharmacopuncture※

New CZE-DAD method for honeybee venom analysis and standardization of the product

Contact Info

Product

Resources

About

Major Components of Clinically used Bee Venom Pharmacopuncture^※