Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: application to clinical study

Spectrophotometric Determination of Fluoxetine by Batch and Flow Injection Methods

“…The results indicate that the proposed method is more rapid, simpler, and more economical than the existing methods, 25,26) provides wider dynamic range, and avoids using toxic organic solvents.…”

Comparison of liquid chromatography with fluorescence detection to liquid chromatography–mass spectrometry for the determination of fluoxetine and norfluoxetine in human plasma

“…Experimental conditions for the iquid chromatography-mass spectrometry (LC-MS) method have been described previously [29], so only details of the liquid chromatographic method with fluorescence detection (LC-FLD) are given here.…”

“…We were able to calculate pharmacokinetic profiles of the drug after single and multiple dosing and consequently to monitor and assess established pharmacotherapy in this specific population of patients. The purpose of this study was to evaluate a simple, sensitive, rapid and highly selective HPLC-FLD method for the determination of FL and NORFL in human plasma and to compare it to a previously established HPLC-MS method [29].…”

“…Our research group has recently developed a sensitive and selective HPLC-MS method [29] used for the analysis of plasma samples from Parkinsonian patients. We were able to calculate pharmacokinetic profiles of the drug after single and multiple dosing and consequently to monitor and assess established pharmacotherapy in this specific population of patients.…”

A simple method to monitor serum concentrations of fluoxetine and its major metabolite for pharmacokinetic studies

“…Though several chromatographic methods have been developed for the determination of FLU and its major metabolite by means of HPLC/UV, HPLC/mass spectrometry or gas chromatography/mass spectrometry; these methods have a limit of quantification (LOQ) that is too high for a pharmacokinetic study [5][6][7]. In addition, some techniques require toxic reagents for derivatization such as (S)-trifluoroacetylpropyl chloride [8] or ethyl chloroformate [9].…”