Liquid chromatographic method for the determination of ticlopidine in human plasma

Róna, Kálmán; Ary, K; Gachályi, B; Klebovich, Imre

doi:10.1016/s0378-4347(97)00082-0

Cited by 13 publications

(10 citation statements)

References 8 publications

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“…Although Ró na et al [8] reported lower recovery than the obtained by Arnoux et al [12], our results are similar to Arnoux values. The formers have used a Bond Elut C 18 solid-phase extraction for the isolation of the compounds from plasma.…”

Section: Discussionsupporting

confidence: 86%

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Determination of ticlopidine in human plasma by capillary gas chromatography with ion trap detection

Pena

Lino

Silveira

2003

Journal of Pharmaceutical and Biomedical Analysis

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A gas chromatographic method for determination of ticlopidine in human plasma using mass spectrometric detection was developed. A Perkin Elmer model 8500 gas chromatograph coupled to an ion trap detector (ITD) in electron impact mode (EI) was used. The gas chromatograph was fitted with a 30 m )/0.32 mm i.d., DB-17 capillary column with a film thickness of 0.25 mm. The compounds were isolated from plasma by Extrelut-1 solid-phase extraction using hexane as the solvent of elution. The detection and quantification limits for this method were 0.005 and 0.01 mg/ml, respectively. The calibration curves were linear from 0.01 and 2.5 mg/ml. Recoveries from human plasma spiked at 0.01 and 2.0 mg/ml ranged from 84.4 and 87.3%. The coefficient of variation (CV) was between 5.1 and 6.9%. The results show that the sensitivity, accuracy and precision of the method are adequate for the determination of ticlopidine in human plasma samples. The ITD in SIM mode allows the unequivocal identification of ticlopidine. The suitability of this method was evaluated in the determination of ticlopidine in plasma from healthy volunteers following oral administration of a dose of 500 mg/day. #

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Section: Discussionsupporting

confidence: 86%

“…Methods based in solid-phase extraction using Extrelut [12] and Bond Elut C 18 columns [8] are also reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of ticlopidine in human plasma by capillary gas chromatography with ion trap detection

Pena

Lino

Silveira

2003

Journal of Pharmaceutical and Biomedical Analysis

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“…The samples were brought to room temperature and dissolved in the mobile phase (10 mM phosphate buffer, pH 5.0 : ACN = 4 : 6) by vortex mixing for 60 s, following the previous method with slight modification (23,24). After filtration with a 0.22-μm Ultrafree ® -MC filter (Millipore Co., Bedford, MA, USA), 100-μl aliquots were injected onto an HPLC apparatus.…”

Section: Hplc Conditionsmentioning

confidence: 99%

Comparison of the Effects of Omeprazole and Rabeprazole on Ticlopidine Metabolism In Vitro

et al. 2011

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Abstract. The thienopyridine derivative ticlopidine (TCL) is an inhibitor of adenosine diphosphate-induced platelet aggregation. Combination therapy with a thienopyridine derivative and aspirin is standard after coronary stenting, although more hemorrhagic complications occur with the combination therapy than with aspirin alone. A proton pump inhibitor (PPI) is required for prevention or treatment of upper gastrointestinal bleeding in such cases. We examined the effects of PPIs [omeprazole (OPZ) and rabeprazole (RPZ)] on TCL metabolism using pooled human liver microsomes prepared from various human liver blocks and 12 individual human liver microsomes. We calculated the K i values of each PPI for TCL metabolic activity and compared the inhibitory effect of each PPI on TCL metabolism. The K i values of OPZ and RPZ were 1.4 and 12.7 μM, respectively. The inhibitory effect of OPZ (78.6 ± 0.05%) was significantly greater than that of RPZ (24.2 ± 0.05%) (P < 0.001). Interestingly, a negative correlation existed between the inhibitory effect of OPZ and CYP2C19 activity (r = −0.909, P < 0.001). These results suggest that the inhibitory effect of OPZ is more potent than that of RPZ in vitro. In conclusion, RPZ appears preferable when administering TCL, aspirin, and a PPI in combination.

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“…UPLC coupled to electrospray tandem mass spectrometry was reported (Borges et al, 2004;Rona et al, 1997). The UPLC method has many advantages over reflectance near-infrared and Fourier transform Raman spectroscopy method for quantitation.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a stability indicating UPLC method for determination of ticlopidine hydrochloride in its tablet formulation

Ram

Kher

L.Dubal

et al. 2011

Saudi Pharmaceutical Journal

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The objective of the current study was the development of a simple, precise and accurate isocratic reversed-phase stability indicating Ultra Performance Liquid Chromatography [UPLC] assay method and validated for determination of ticlopidine hydrochloride in solid pharmaceutical dosage forms. Isocratic separation was achieved on a Zorbax SB-C18 (50 mm × 4.6 mm, 1.8 μm) column using mobile phase of methanol-0.01 M ammonium acetate buffer, pH 5.0 (80:20, v/v) at a flow rate of 0.8 ml min(-1), the injection volume was 4.0 μl and the detection was carried out at 235 nm by using photo-diode array detector. The drug was subjected to oxidation, hydrolysis, photolysis and heat to apply stress condition. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was linear in the drug concentration range of 62.5-375 μg ml(-1) with a correlation coefficient of 0.9999. The precision (relative standard deviation - RSD) of six samples was 1.31% for repeatability and the intermediate precision [RSD] among six-sample preparation was 0.77%. The accuracy (recovery) was between 98.80% and 101.50%. Degradation products produced as a result of stress studies did not interfere with detection of ticlopidine hydrochloride and the assay can thus be considered stability indicating.

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Liquid chromatographic method for the determination of ticlopidine in human plasma

Cited by 13 publications

References 8 publications

Determination of ticlopidine in human plasma by capillary gas chromatography with ion trap detection

Determination of ticlopidine in human plasma by capillary gas chromatography with ion trap detection

Comparison of the Effects of Omeprazole and Rabeprazole on Ticlopidine Metabolism In Vitro

Development and validation of a stability indicating UPLC method for determination of ticlopidine hydrochloride in its tablet formulation

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