Liquid Chromatographic Method for the Determination of Benzo[<i>a</i>]pyrene in Filter Tar of Turkish Cigarettes

Aygün, S. Fatma; Demirci, and Ayla; Özcimder, Mustafa

doi:10.1021/jf940716b

Cited by 9 publications

(6 citation statements)

References 13 publications

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“…The main reason for low recovery is generally attributed to the complex composition of the tar mixture. Although the used solvent (dichloromethane) was the best choice for such an extraction, the enhancement of the recovery could not have been achieved [12]. The n-hexane extract of cigarette filters consists of a complex mixture of components.…”

Section: Extraction and Clean-upmentioning

confidence: 99%

Determination of some polycyclic aromatic hydrocarbons in filter tar of Turkish cigarettes

Aygün¹

2005

J of Separation Science

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A simultaneous determination method for the analysis of benzo[a]pyrene (BaP) and dibenzo[a,h]anthracene (DahA) in the filter tar of Turkish cigarettes has been developed. The method involved (a) the extraction of BaP and DahA with n-hexane from ACN solution in which the cigarette filters were extracted, and then (b) purification of the n-hexane extracts by elution on an XAD-2 column using n-hexane/dichloromethane (9:1, v/v) mixture. Separation and quantitative determination of BaP and DahA in the extracts were carried out by HPLC and fluorescence detection on a C18 RP column. The calculated recoveries for BaP and DahA were found in the range of 90-100% for each extraction and clean-up steps. Analysis of various filter tar of Turkish cigarettes showed that an average of 74.28 ng/filter of BaP and 5.24 ng/filter of DahA were present in Turkish cigarettes.

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Section: Extraction and Clean-upmentioning

confidence: 99%

Determination of some polycyclic aromatic hydrocarbons in filter tar of Turkish cigarettes

Aygün¹

2005

J of Separation Science

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“…Five brands of cigarettes (Cg1, Cg2, Cg3, Cg4 and Cg5) were bought randomly from the market, with the specifications given in Table-1. The analytical procedure described previously to determine benzo(a)pyrene 4 in filter tar was also applied here. Five cigarettes of each type were smoked by means of a standard smoking machine.…”

Section: Methodsmentioning

confidence: 99%

“…Since the smokers inhale the smoke into their body, most of the research focus on smoke analysis 1 . However, the filter (butt) is continuously in touch with the smoker's lips and consequently they have the chance that some hazardous compounds present in filter tar 2,3 , for instance benzo(a)pyrene [4][5][6][7] may enter into the body. That means, filter residue analyses deserves equal attention as smoke analysis.…”

Section: Introductionmentioning

confidence: 99%

Statistical Classification of Cigarettes Using High Pressure Liquid Chromatograms of Cigarette Filter Tar as Fingerprint

Demirci¹,

Ekiz²

2013

Asian J. Chem.

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In this work, we have showed that a liquid chromatogram can be used as finger print to characterize and classify cigarettes. A liquid chromatographic (high pressure liquid chromatography) procedure was first developed to obtain chromatograms of different cigarettes. The cellulose filter of a smoked cigarette was first dissolved in 5 mL acetonitrile. Then the organics were partitioned into the n-hexane phase. After concentrating the n-hexane phase to 100 µL, 5 µL was injected to a high pressure liquid chromatography. The chromatograms were obtained using a C18 reversed phase column. The mobile phase was 80 % acetonitrile/water with 1 mL min-1. The detector was UV spectrometer working at 254 nm. For the evaluation the chromatograms as fingerprints, five peaks were chosen randomly. The heights of the corresponding peaks were used for the statistical evaluation which was based on the discriminate analysis for classifying problems introduced by Anderson. To achieve this, a Matlab computer program was developed. The proposed method can be performed in a relatively short time.

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“…Reverse phase chromatography is preferred over normal phase in pharmaceutical analysis as most of the drugs can be retained by this phase. In addition water is used in reverse phase chromatography which is also an advantage [14]. The purpose of stability testing is to check the drug quality under the action of many environmental factors like temperature, acid, base and oxidative condition.…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating RP-HPLC method for simultaneous determination of methoxsalen and p-aminobenzoic acid in binary combination

John¹,

Azeem²,

Ashfaq³

et al. 2015

Bull. Chem. Soc. Eth.

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ABSTRACT. An accurate, simple and specific reverse phase LC procedure is established and validated for simultaneous estimation of methoxsalen and p-aminobenzoic acid in pharmaceutical formulated products and human serum. Chromatographic separation among methoxsalen, p-aminobenzoic acid and their degradation products have been achieved in less than 5 min with Hypersil-ODS column (250 mm x 4.6 mm, 5 µm), using acetonitrile and 1.28 mM phosphate buffer as mobile phase (60:40 v/v). Flow rate of the mobile phase was set as 1.5 mL min -1 and detection of all the analytes was carried out by diode-array detector at 254 nm. The developed method was validated according to ICH guidelines by performing its linearity, accuracy, precision, specificity, robustness and LOD/LOQ values. Response was linear and proportional to the concentrations (24-48 µg mL -1 ) for p-aminobenzoic acid and (9-18 µg mL -1 ) for methoxsalen. The LOD was 2.3 ng mL -1 for p-aminobenzoic acid and 6 ng mL -1 for methoxsalen whereas LOQ was 7.8 ng mL -1 for p-aminobenzoic acid and 20.4 ng mL -1 for methoxsalen. The developed method efficiently separated the principal peaks from degradation products and therefore can be applied successfully for concurrent analysis of methoxsalen and p-aminobenzoic acid in pharmaceutical dosage form, human serum and product stability studies.

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Liquid Chromatographic Method for the Determination of Benzo[a]pyrene in Filter Tar of Turkish Cigarettes

Cited by 9 publications

References 13 publications

Determination of some polycyclic aromatic hydrocarbons in filter tar of Turkish cigarettes

Determination of some polycyclic aromatic hydrocarbons in filter tar of Turkish cigarettes

Statistical Classification of Cigarettes Using High Pressure Liquid Chromatograms of Cigarette Filter Tar as Fingerprint

Stability-indicating RP-HPLC method for simultaneous determination of methoxsalen and p-aminobenzoic acid in binary combination

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