Liquid chromatography method with tandem mass spectrometry and fluorescence detection for determination of inflammatory biomarkers in gingival crevicular fluid as a tool for diagnosis of periodontal disease

Vernerová, Andrea; Krčmová, Lenka Kujovská; Heneberk, Ondřej; Radochová, Vladimíra; Švec, František

doi:10.1016/j.jpba.2022.114644

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…Neopterin was evaluated in various body fluids, including [10,51], urine, mixed saliva (oral fluid) [10,52,53], gingival crevicular fluid [44,53,54], synovial fluid [55], ascitic fluid [56], cerebrospinal fluid [13], amniotic fluid [57], and pus [58].…”

Section: Neopterin As a Biomarkermentioning

confidence: 99%

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Neopterin, the Cell-Mediated Immune Response Biomarker, in Inflammatory Periodontal Diseases: A Narrative Review of a More than Fifty Years Old Biomarker

2023

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Neopterin is a biomarker of the activation of cellular immunity. The purpose of this review is to summarise neopterin metabolism, methods of its detection, and its role in inflammation, focusing on periodontal inflammatory diseases. This derivative of guanosine is a non-enzymatic product of 7,8-dihydroneopterin oxidation caused by free radicals which protect activated macrophages from oxidative stress. Various methods, usually based on enzyme-linked immunosorbent essay, high-performance liquid chromatography, or radioimmunoassay were developed for the isolation of neopterin. A wide spectrum of diseases and conditions are known to affect neopterin levels, including cardiovascular, bacterial, viral, and degenerative diseases, as well as malignant tumours. Neopterin levels were found to increase in subjects with periodontitis, especially when the oral fluid and gingival crevicular fluid were evaluated. These findings confirm the role of activated macrophages and cellular immunity in periodontal inflammatory diseases. The gingival crevicular fluid and the oral fluid appear to be the most valuable biologic fluids for the evaluation of neopterin levels in periodontitis. For gingival crevicular fluid, neopterin can be determined as the concentration or the so-called total amount. Nonsurgical periodontal treatment was associated with a decrease in neopterin levels, but an increase was also reported, suggesting the possible role of macrophages in the resolution of the periodontal lesion.

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Section: Neopterin As a Biomarkermentioning

confidence: 99%

“…Np in gingival crevicular fluid was also detected by commercially available ELISA kits. Only one HPLC method has been developed for the simultaneous analysis of Np in conjunction with creatinine, kynurenine, and tryptophan [54].…”

Section: Neopterin As a Biomarkermentioning

confidence: 99%

Neopterin, the Cell-Mediated Immune Response Biomarker, in Inflammatory Periodontal Diseases: A Narrative Review of a More than Fifty Years Old Biomarker

2023

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“…Historically, sample purification has normally been performed off-chip, using affinity resins on solid supports such as magnetic beads, 42,43 or chromatography columns. [44][45][46] For more complex samples, such as whole blood, a centrifugation step is often employed to separate the target from the matrix. [47][48][49][50] A great deal of effort has been dedicated to moving these processes to microfluidic formats.…”

Section: Sample Processingmentioning

confidence: 99%

Simplifying the complex: accessible microfluidic solutions for contemporary processes within in vitro diagnostics

et al. 2022

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“…Samples contaminated with saliva or blood were discarded. The GCF samples were analyzed by the newly developed method, described previously in detail [25]. The amount of each GCF sample was obtained by calculating the weight difference of the retraction cord sealed in an Eppendorf tube before and immediately after the collection of GCF.…”

Section: Sample Collection Preparation and Analysismentioning

confidence: 99%

“…Upon transportation and centrifugation, the samples were stored at −80 °C. The sample preparation technique was carried out under optimized conditions using The GCF samples were analyzed by the newly developed method, described previously in detail [25]. The amount of each GCF sample was obtained by calculating the weight difference of the retraction cord sealed in an Eppendorf tube before and immediately after the collection of GCF.…”

Section: Sample Collection Preparation and Analysismentioning

confidence: 99%

Neopterin Levels in Periodontitis and after Nonsurgical Periodontal Therapy: Evaluation of Gingival Crevicular Fluid, Oral Fluid, Serum and Urinary Samples—A Case-Control Study

et al. 2022

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Periodontitis is a chronic inflammatory disease that leads to the destruction of the tooth-supporting tissues with complex immune response. Neopterin (Np), secreted via activated macrophages, is considered a biomarker of cellular immunity. The aim of this study was to evaluate the impact of periodontitis and nonsurgical periodontal therapy. Np gingival crevicular fluid (GCF), oral fluid, serum and urine levels were compared in subjects with periodontitis before periodontal treatment, three months after and in a healthy control. Np GCF concentrations in the study group after treatment were significantly higher than the control group (p = 0.038). The GCF total amount (amount of substance) was significantly higher in the study group before periodontal treatment than in the control group (p = 0.001) and higher than the levels taken after treatment collection (p = 0.024). The oral fluid Np concentrations in the study group after treatment were significantly increased compared to the before treatment concentrations (p = 0.020). The same trend was observed in the urine samples. Significant correlation was found between the serum and oral fluid Np concentrations (p = 0.001, ρ = 0.40). Our results confirm the impact of cellular immunity and macrophages on periodontitis and on the resolution of periodontal inflammation. The presence of neopterin in oral fluid most likely originates in the serum.

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Liquid chromatography method with tandem mass spectrometry and fluorescence detection for determination of inflammatory biomarkers in gingival crevicular fluid as a tool for diagnosis of periodontal disease

Cited by 6 publications

References 27 publications

Neopterin, the Cell-Mediated Immune Response Biomarker, in Inflammatory Periodontal Diseases: A Narrative Review of a More than Fifty Years Old Biomarker

Neopterin, the Cell-Mediated Immune Response Biomarker, in Inflammatory Periodontal Diseases: A Narrative Review of a More than Fifty Years Old Biomarker

Simplifying the complex: accessible microfluidic solutions for contemporary processes within in vitro diagnostics

Neopterin Levels in Periodontitis and after Nonsurgical Periodontal Therapy: Evaluation of Gingival Crevicular Fluid, Oral Fluid, Serum and Urinary Samples—A Case-Control Study

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