Liquid chromatography–tandem mass spectrometric method for determination of mosapride citrate in equine tissues

Aoki, Yoichi; Hakamata, Hideki; Igarashi, Yu; Uchida, Kazunari; Kobayashi, Hisato; Hirayama, Norio; Kotani, Akio; Kusu, Fumiyo

doi:10.1016/j.jchromb.2007.08.017

Cited by 11 publications

(13 citation statements)

References 20 publications

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“…Separation of mosapride in HPLC has been accomplished mainly by reverse-phase C 18 columns with mobile phases consist of acetonitrile/methanol-acidic aqueous buff ers (Table 4) (Aoki et al, 2007;Qin et al, 2007;Ramakrishna et al, 2005;Yokoyama et al, Table 2. Recovery of mosapride in spiked plasma (mean ± SD, n = 4) Concentration Absolute recovery (%) Relative recovery (%) (μg/mL) Mosapride Cisapride Mosapride/cisapride 0.025 99.4 ± 5.9 98.6 ± 4.0 100.9 ± 8.0 0.5 102.7 ± 4.1 96.8 ± 2.8 106.2 ± 4.5 5 96.8 ± 4.2 99.5 ± 1.0 97.4 ± 5.6 1997).…”

Section: Methods Developmentmentioning

confidence: 99%

“…Ramakrishna et al (2005) found that the addition of formic acid in mobile phase was necessary to increase the acidity for protonization of mosapride and for obtaining good peak shape for LC-MS/MS with a conventional C 18 column. The strategy was also employed in other LC-MS/MS methods using microbore and UPLC columns (Aoki et al, 2007;Qin et al, 2007;Sun et al, 2009). The use of gradient elution was recommended as it gave good peak shape and better sensitivity for mosapride and less contamination buildup (Aoki et al, 2007).…”

Section: Methods Developmentmentioning

confidence: 99%

“…Because of little arrhythmic eff ect associated with the QT prolongation, mosapride has been used increasingly in Japan, Taiwan and some other Asian countries (Curran and Robinson, 2008). And the potential of mosapride to be used in the fi eld of veterinary medicine and animal science has also been proposed recently (Aoki et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Animal models such as rats (Aoki et al, 2007), guinea pigs (Kojima et al, 2006) and mice (Asakawa et al, 2006) are commonly used in the pharmacokinetic and pharmacological studies of mosapride to explore its clinical eff ects. To investigate these issues, a sensitive assay that requires small sample volume for measuring mosapride in small animals such as rats is necessary.…”

Section: Introductionmentioning

confidence: 99%

“…Another LC-MS/MS method was reported to measure mosapride levels in 5 g of various equine tissues (Aoki et al, 2007). A new UPLC-MS/MS method has just been published to quantify mosapride in rat plasma, bile and urine (0.6 mL) and feces (0.2 g) for the characterization of metabolic profi le of mosapride in rats (Sun et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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HPLC‐fluorescence assay for measuring mosapride in small volumes of rat plasma

Cheng

Chang

Chou

2009

Biomedical Chromatography

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A simple and sensitive HPLC-fluorescence assay was developed for the determination of a gastroprokinetic agent mosapride in small volumes of rat plasma. Samples (50 microL) were treated with 200 microL of the internal standard solution (cisapride, 0.1 microg/mL in acetonitrile). Chromatographic separation was achieved on a C(18) column by gradient elution with the mobile phase of acetonitrile-water containing 20 mM potassium dihydrogen phosphate, at a flow rate of 1 mL/min. Fluorescence was measured with excitation and emission set at 315 and 354 nm, respectively. The retention time was about 16 min for cisapride and 20 min for mosapride. No endogenous substances were found to interfere. The calibration curve was linear from 0.015 to 10 microg/mL. The lower limit of quantification was 0.015 microg/mL. The intra- and inter-day precision expressed as relative standard deviation did not exceed 7.7%, and the accuracy was within 4.7% deviation of the nominal concentration. The method was used successfully to investigate the disposition kinetics of mosapride in rats.

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Section: Methods Developmentmentioning

confidence: 99%

Section: Methods Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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HPLC‐fluorescence assay for measuring mosapride in small volumes of rat plasma

Cheng

Chang

Chou

2009

Biomedical Chromatography

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Stability‐indicating methods for the determination of mosapride citrate in the presence of its degradation products according to ICH guidelines

Hegazy¹,

Yehia²,

Mostafa³

2011

Drug Testing and Analysis

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In the present work, different spectrophotometric methods and one spectrofluorimetric method have been developed and validated for the determination of mosapride citrate in the presence of its acid-induced degradation products. The drug was subjected to stress stability study including acid, alkali, oxidative, photolytic, and thermal stress degradation. The developed spectrophotometric methods included the use of first order derivative ((1)D), derivative of ratio spectra ((1)DD), mean centring of ratio spectra (MC) and H-point standard additions (HPSAM) spectrophotometric methods. For (1)D method, the peaks amplitudes at 282.8 and 319.6 nm were measured, while for (1)DD method those at 308 nm and 323 nm were measured. Mean centring of ratio spectra method used the values at 317 nm for calibration, while for HPSAM the absorbance at 273 and 288.6 nm were used. These methods were successfully applied for determination of mosapride in the concentration range of 5-70 µg.ml(-1). The spectrofluorimetric method was based on measuring the native fluorescence of mosapride in 0.1 M NaOH using λ(excitation) 276 nm and λ(emission) 344 nm and 684 nm with linearity ranges of 50-3000 ng.ml(-1) and 50-9000 ng.ml(-1), respectively. All the developed methods were validated according to the International Conference on Harmonization (ICH) guidelines and were applied for bulk powder and dosage form. The results obtained were statistically compared to each other using one-way ANOVA testing.

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Current literature in mass spectrometry

2008

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Reviews; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 27th. Feb. 2008)

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Liquid chromatography–tandem mass spectrometric method for determination of mosapride citrate in equine tissues

Cited by 11 publications

References 20 publications

HPLC‐fluorescence assay for measuring mosapride in small volumes of rat plasma

HPLC‐fluorescence assay for measuring mosapride in small volumes of rat plasma

Stability‐indicating methods for the determination of mosapride citrate in the presence of its degradation products according to ICH guidelines

Current literature in mass spectrometry

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