Liquid chromatography–tandem MS/MS method for simultaneous quantification of paracetamol, chlorzoxazone and aceclofenac in human plasma: An application to a clinical pharmacokinetic study

Abstract

A facile, fast and specific method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous quantitation of paracetamol, chlorzoxazone and aceclofenac in human plasma was developed and validated. Sample preparation was achieved by liquid-liquid extraction. The analysis was performed on a reversed-phase C HPLC column (5 μm, 4.6 × 50 mm) using acetonitrile-10 mM ammonium formate pH 3.0 (65:35, v/v) as the mobile phase where atrovastatin was used as an internal standard. A very small injection volume (3 μL) was applied and the run time was 2.0 min. The detection was carried out by electrospray positive and negative ionization mass spectrometry in the multiple-reaction monitoring mode. The developed method was capable of determining the analytes over the concentration ranges of 0.03-30.0, 0.015-15.00 and 0.15-15.00 μg/mL for paracetamol, chlorzoxazone and aceclofenac, respectively. Intraday and interday precisions (as coefficient of variation) were found to be ≤12.3% with an accuracy (as relative error) of ±5.0%. The method was successfully applied to a pharmacokinetic study of the three analytes after being orally administered to six healthy volunteers.

Sensitive and selective determination of paracetamol in antipyretic children's syrup with a polyglycine modified glassy carbon electrode

“…Specicity and sensitivity are the most important parameters that help identify target analytes. 5 According to articles that have been published until now there are different advanced, rened and sensitive methods that have been applied for the determination of ACOP i.e., titrimetric, [13][14][15] colorimetry, [16][17][18][19] spectrophotometry and spectroscopy, [20][21][22][23] gas chromatography, [24][25][26][27][28] near-infrared transmittance spectroscopy, [29][30][31][32] high performance liquid chromatography (HPLC), [33][34][35] chemiluminescence, 36-39 uorescence, [40][41][42][43] liquid chromatography tandem mass spectrometry, [44][45][46] capillary electrophoresis 47,48 and thin-layer chromatography (TLC). 49,50 In general, these methods are not suitable for routine analysis of ACOP because they require expensive instruments or samples require pretreatment steps before analysis.…”

A Modest Uv Spectrophotometric Assisted by Chemometric Approach for Verification of Acetaminophen Level in Various Manufactured Tablets and Syrups in Indonesian Pharmacies

“…Consideration of a suitable method is required, such as fast, modest, inexpensive, and at the same time, the method does not influence the reliability, precision, and accuracy of the results. The studies have unveiled numerous techniques for the detection of drugs, whether in single or multicomponents [7][8][9]. Furthermore, only HPLC and UV approaches can be applied for the determination of paracetamol [10][11][12][13][14].…”

Differential Pulse Anodic Voltammetric Determination of Chlorzoxazone in Pharmaceutical Formulation using Carbon Paste Electrode

“…It is used as an adjunct in the symptomatic treatment of painful muscle spasm. 1,2 phy, [3][4][5][6][7][8][9][10][11][12][13] thin layer chromatography, [14][15][16][17] liquid chromatography-tandem mass spectrometry, 18 packed column supercritical fluid chromatography, [19][20] gas chromatography, [21][22][23] spectrophotometry, [24][25][26][27][28][29][30][31][32][33][34][35] fluorimetry, 36 and capillary zone electrophoresis. 37 Two papers have been described in literature concerning the voltammetric determination of chlorzoxazone based on the oxidation of the drug at the glassy carbon electrode and gold electrode.…”