Liquid chromatography with tandem mass spectrometry method for the simultaneous determination of multiple sweet mogrosides in the fruits of<i>Siraitia grosvenorii</i>and its marketed sweeteners

Luo, Zuliang; Shi, Hongwu; Zhang, Kailun; Qin, Xijun; Guo, Yu‐Hua; Ma, Xiaojun

doi:10.1002/jssc.201600563

Cited by 19 publications

(17 citation statements)

References 31 publications

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“…The MV content accounts for 45.23–63.58% of the total content of the 21 targeted analytes. This result is consistent with previous reports that MV was found in a sample of whole fruits of S. grosvenorii at levels of 49.29–66.96% [27]. Moreover, its content was in the range of 4.86–13.49 mg/g in 15 varieties of luohanguo samples, all of whose contents, except the S10 variety, were all higher than the 5 mg/g set in the Chinese Pharmacopeia.…”

Section: Resultssupporting

confidence: 92%

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Identification of a Novel Specific Cucurbitadienol Synthase Allele in Siraitia grosvenorii Correlates with High Catalytic Efficiency

Qiao

Luo

et al. 2019

Molecules

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Mogrosides, the main bioactive compounds isolated from the fruits of Siraitia grosvenorii, are a group of cucurbitane-type triterpenoid glycosides that exhibit a wide range of notable biological activities and are commercially available worldwide as natural sweeteners. However, the extraction cost is high due to their relatively low contents in plants. Therefore, molecular breeding needs to be achieved when conventional plant breeding can hardly improve the quality so far. In this study, the levels of 21 active mogrosides and two precursors in 15 S. grosvenorii varieties were determined by HPLC-MS/MS and GC-MS, respectively. The results showed that the variations in mogroside V content may be caused by the accumulation of cucurbitadienol. Furthermore, a total of four wild-type cucurbitadienol synthase protein variants (50R573L, 50C573L, 50R573Q, and 50C573Q) based on two missense mutation single nucleotide polymorphism (SNP) sites were discovered. An in vitro enzyme reaction analysis indicated that 50R573L had the highest activity, with a specific activity of 10.24 nmol min−1 mg−1. In addition, a site-directed mutant, namely, 50K573L, showed a 33% enhancement of catalytic efficiency compared to wild-type 50R573L. Our findings identify a novel cucurbitadienol synthase allele correlates with high catalytic efficiency. These results are valuable for the molecular breeding of luohanguo.

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Section: Resultssupporting

confidence: 92%

“…The developed HPLC-MS/MS method was applied to determine the levels of the 21 mogrosides in the fruits of different S. grosvenorii varieties. The quantitative analyses were performed by means of an external standard method [27]. The results are summarized in Table A2, and a graphical representation of the results is shown in Figure 2.…”

Section: Resultsmentioning

confidence: 99%

Identification of a Novel Specific Cucurbitadienol Synthase Allele in Siraitia grosvenorii Correlates with High Catalytic Efficiency

Qiao

Luo

et al. 2019

Molecules

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“…The mass spectra of positive and negative ion modes were compared under full scan mode to choose the optimum ionization mode and identify the parent ions of the targeted analytes. At the same time, the important MS parameter declustering potential (DP) was optimized to obtain the maximum sensitivity [22]. With the selection of precursor ions, product ion scanning and MRM scanning were conducted to obtain specific fragment ions and the collision energies (CEs) in favor of highest response and least interference.…”

Section: Resultsmentioning

confidence: 99%

Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas

et al. 2019

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Currently, paclobutrazol is excessively used in the planting process of Ophiopogon japonicus (O. japonicus) due to its important role in regulating the growth of tuber roots, ultimately increasing the yield and shortening the growth cycle of Ophiopogonis Radix. For insight into this process and the potential risks of paclobutrazol and its mediated consequences on the secondary metabolites in Ophiopogonis Radix, corresponding high performance liquid chromatography-tandem mass spectrometric methods (HPLC-MS/MS) were developed in this study and then applied to Ophiopogonis Radix, soil, and water samples. The results demonstrated the detection of different levels of paclobutrazol residue were in Ophiopogonis Radix, soil, and water samples. In addition, the quantitative results of the secondary metabolites showed that paclobutrazol significantly decreased four steroidal saponins in Ophiopogonis Radix, especially ophiopogonin D, where the content was decreased from 824.87 to 172.50 mg/kg. Concurrently, ophiopogonanone C, a flavonoid in Ophiopogonis Radix, also significantly decreased from 2.66 to 1.33 mg/kg. In conclusion, the residual paclobutrazol and its negative regulation on the secondary metabolism of Ophiopogonis Radix brings potential hazards to the environment and human health. These results provide more comprehensive data that can be used for the reassessment of the use of paclobutrazol in O. japonicus and the formulation of related standards.

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“…It has the reputation of "Oriental fruit" and "Longevity fruit" in Southeast Asia and Western countries, and it is also a traditional specialty in northern Guangxi province of China [1,2]. To date, several classes of compounds were isolated from LHG such as triterpenoids (especially mogroside), flavonoids, vitamins, amino acid, saccharides, volatile oil and so on.…”

Section: Introductionmentioning

confidence: 99%

Microwave drying of Siraitia grosvenorii and its effect on the Vitamin C content

Zhou¹,

Huang²,

Li³

2018

Proceedings of the 2018 7th International Conference on Energy, Environment and Sustainable Development (ICEESD 2018)

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Siraitia grosvenorii (Luo hanguo, LHG) is a unique edible and medicinal plant in China. Drying the LHG with a rapid, highly efficient method and keeping good quality are urgent problems. In this paper, the changes of Vitamin C (VC) content in LHG under microwave drying were studied. The effects of key drying parameters (microwave power, drying time and microwave intermittent ratio) on the quality of LHG have been investigated by orthogonal experiments to reduce the loss of VC in the process of microwave drying. The optimum drying parameters for drying LHG were microwave power of 80 W, drying time of 30 min and microwave intermittent ratio of 1: 1. The optimally treated LHG has 34.072 mg/100g of VC content. These results have indicated that the microwave-heating not only prolongs the storage duration of the LHG but also reduces the moisture content and VC content, while the natural yellow-green color of the LHG is not affected much.

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Liquid chromatography with tandem mass spectrometry method for the simultaneous determination of multiple sweet mogrosides in the fruits ofSiraitia grosvenoriiand its marketed sweeteners

Cited by 19 publications

References 31 publications

Identification of a Novel Specific Cucurbitadienol Synthase Allele in Siraitia grosvenorii Correlates with High Catalytic Efficiency

Identification of a Novel Specific Cucurbitadienol Synthase Allele in Siraitia grosvenorii Correlates with High Catalytic Efficiency

Residue of Paclobutrazol and Its Regulatory Effects on the Secondary Metabolites of Ophiopogon japonicas

Microwave drying of Siraitia grosvenorii and its effect on the Vitamin C content

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