Liquid chromatography with tandem mass spectrometry-based proteomic discovery in aging and Alzheimer’s disease

Montine, Thomas J.; Woltjer, Randall L.; Pan, Catherine; Montine, Kathleen S.; Zhang, Jing

doi:10.1016/j.nurx.2006.05.002

Cited by 20 publications

(8 citation statements)

References 41 publications

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“…In addition, a variety of proinflammatory molecules, including cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α), and inducible nitric oxide synthase (iNOS), produced by activated glial cells, are elevated in the brains of AD transgenic animal models and patients. Moreover, another proinflammatory molecule, prostaglandin E2 (PGE2), is increased in the cerebrospinal fluid (CSF) of AD patients [1]. These proinflammatory molecules, which can be released by activated astrocytes, contribute to neuronal damage and accelerate the progression of neurodegenerative disorders.…”

Section: Introductionmentioning

confidence: 99%

3-N-Butylphthalide (NBP) Attenuates the Amyloid-�-Induced Inflammatory Responses in Cultured Astrocytes via the Nuclear Factor-κB Signaling Pathway

Wang

Zhang

Huang

et al. 2013

Cell Physiol Biochem

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Background/Aims: Activation of astrocytes is a common feature of Alzheimer's disease (AD). Proinflammatory molecules produced by activated astrocytes contribute to neuronal damage in AD. Moreover, dl-3-n-butylphthalide (NBP) has been reported to attenuate astroglial activation and exert neuroprotective effects in AD transgenic mice. However, the mechanism by which NBP inhibits activated astrocytes is poorly understood. Methods: In this study, the primary astrocytes were obtained from the cerebral cortices of 1-day-old Sprague-Dawley rats. The levels of GFAP, COX-2, NF-κB, and IκBa were examined by Western blotting and the levels of TNF-a and IL-6 were determined by ELISA. Results: NBP inhibited the amyloid-ß (Aß)-induced activation of astrocytes and the up-regulation of proinflammatory molecules. Importantly, NBP markedly suppressed Aß-induced IκBa degradation and nuclear factor-κB (NF-κB) translocation. Conclusion: Our results suggest that NBP attenuates Aß-induced activation of astrocytes and neuroinflammation via inhibition of the NF-κB signaling pathway.

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Section: Introductionmentioning

confidence: 99%

3-N-Butylphthalide (NBP) Attenuates the Amyloid-�-Induced Inflammatory Responses in Cultured Astrocytes via the Nuclear Factor-κB Signaling Pathway

Wang

Zhang

Huang

et al. 2013

Cell Physiol Biochem

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“…Thus, identification of proteins unique to each brain region, those associated with neurodegenerative mechanisms in particular, could yield opportunities to overcome major obstacles in the development of new protective and restorative therapies for prominent neurodegenerative diseases. As an emerging technology, MS-based proteomics is increasingly used for this purpose (3)(4)(5)(6)(7)(8)(9)(10).…”

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confidence: 99%

Proteomics Identification of Proteins in Human Cortex Using Multidimensional Separations and MALDI Tandem Mass Spectrometer

Pan

Shi

Jin

et al. 2007

Molecular & Cellular Proteomics

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It is essential to characterize the proteome of various regions of human brain because most, if not all, neurodegenerative diseases are region-specific. Here we report an in-depth proteomics identification of proteins extracted from the frontal cortex, a region playing a critical role in cognitive function. The integrated proteomics analytical flow consisted of biochemical fractionation, strong cation exchange chromatography, reverse phase liquid chromatography, and MALDI-TOF/TOF mass spectrometric analysis. In total, 812 proteins were confidently identified with two or more peptides. These proteins demonstrated diverse isoelectric points and molecular weights and are involved in several molecular functions, including protein binding, catalytic activity, transport, structure, and signal transduction. A number of proteins known to be associated with neurodegenerative diseases were also identified. Detailed characterization of these proteins will supply the necessary information to appropriately interpret proteins associated with aging and/or age-related neurodegenerative diseases. Finally 140 proteins found in the cortical proteome were present in the proteome of cerebrospinal fluid, providing tissue-specific candidates for biomarker discovery in body fluid.

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“…Clinical applications of MS‐based multiplex protein quantification have recently been reviewed and are summarized in Table . Quantitative proteomics in chronic kidney disease , cardiovascular disease , cancer , and diseases of the CNS including Alzheimer and psychiatric disorder have already been reviewed in detail.…”

Section: Applicationsmentioning

confidence: 99%

Clinical applications of MS‐based protein quantification

Sabbagh

Mindt

Neumaier

et al. 2016

Proteomics Clinical Apps

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Mass spectrometry-based assays are increasingly important in clinical laboratory medicine and nowadays are already commonly used in several areas of routine diagnostics. These include therapeutic drug monitoring, toxicology, endocrinology, pediatrics, and microbiology. Accordingly, some of the most common analyses are therapeutic drug monitoring of immunosuppressants, vitamin D, steroids, newborn screening, and bacterial identification. However, MS-based quantification of peptides and proteins for routine diagnostic use is rather rare up to now despite excellent analytical specificity and good sensitivity. Here, we want to give an overview over current fit-for-purpose assays for MS-based protein quantification. Advantages as well as challenges of this approach will be discussed with focus on feasibility for routine diagnostic use.

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Liquid chromatography with tandem mass spectrometry-based proteomic discovery in aging and Alzheimer’s disease

Cited by 20 publications

References 41 publications

3-N-Butylphthalide (NBP) Attenuates the Amyloid-�-Induced Inflammatory Responses in Cultured Astrocytes via the Nuclear Factor-κB Signaling Pathway

3-N-Butylphthalide (NBP) Attenuates the Amyloid-�-Induced Inflammatory Responses in Cultured Astrocytes via the Nuclear Factor-κB Signaling Pathway

Proteomics Identification of Proteins in Human Cortex Using Multidimensional Separations and MALDI Tandem Mass Spectrometer

Clinical applications of MS‐based protein quantification

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