Liquid extraction surface analysis nanospray electrospray ionization based lipidomics for in situ analysis of tumor cells with multidrug resistance

Zong, Li; Pi, Zifeng; Liu, Shu; Xing, Junpeng; Liu, Zhiqiang; Song, Fengrui

doi:10.1002/rcm.8229

Cited by 16 publications

(10 citation statements)

References 33 publications

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“…Another group identified 24 biomarkers and found the pathways of protein and cysteine synthesis, glutamine metabolism and the ammonia cycle were related to drug resistance by using MCF-7/ADR and its parental MCF-7/S cells with ultra-performance liquid chromatography-tandem mass spectrometry 75. Moreover, the ratios of GSH/GSSG, ATP/ADP and ATP/AMP were prominently higher in MCF-7/ADR cells compared to MCF-7/S cells 76. Statins are considered to have antitumour potential in recent years 77.…”

Section: The Application Of Metabolomics In the Clinical Practice Of Bcmentioning

confidence: 99%

Metabolomics: a promising diagnostic and therapeutic implement for breast cancer

Chen¹,

Li²,

Li³

et al. 2019

OTT

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Breast cancer (BC) is the most commonly diagnosed cancer among women and the leading cause of cancer death. Despite the advent of numerous diagnosis and treatment methods in recent years, this heterogeneous disease still presents great challenges in early diagnosis, curative treatments and prognosis monitoring. Thus, finding promising early diagnostic biomarkers and therapeutic targets and approaches is meaningful. Metabolomics, which focuses on the analysis of metabolites that change during metabolism, can reveal even a subtle abnormal change in an individual. In recent decades, the exploration of cancer-related metabolomics has increased. Metabolites can be promising biomarkers for the screening, response evaluation and prognosis of BC. In this review, we summarized the workflow of metabolomics, described metabolite signatures based on molecular subtype as well as reclassification and then discussed the application of metabolomics in the early diagnosis, monitoring and prognosis of BC to offer new insights for clinicians in breast cancer diagnosis and treatment.

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Section: The Application Of Metabolomics In the Clinical Practice Of Bcmentioning

confidence: 99%

Metabolomics: a promising diagnostic and therapeutic implement for breast cancer

Chen¹,

Li²,

Li³

et al. 2019

OTT

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“…179 Zong et al have employed LESA-MS for the in situ analysis of lipids in breast cancer cells dried on glass microscope slides, permitting the characterization of multidrug resistance based on changes in cellular lipid profiles. 180 The integration of atomic force microscopy for imaging cell morphology prior to and postanalysis indicated structural differences of cells post-LESA sampling, revealing the effective extraction of lipids from the cells. Another study profiled lipids in mycobacterial cells directly from colonies on agar media.…”

Section: ■ Innovative Sampling Methodsmentioning

confidence: 99%

“…of 1 mm) and the sample surface, which is aspirated toward a nanoESI source, facilitating the spot sampling of metabolites and lipids directly from the cell cultures . Zong et al have employed LESA-MS for the in situ analysis of lipids in breast cancer cells dried on glass microscope slides, permitting the characterization of multidrug resistance based on changes in cellular lipid profiles . The integration of atomic force microscopy for imaging cell morphology prior to and postanalysis indicated structural differences of cells post-LESA sampling, revealing the effective extraction of lipids from the cells.…”

Section: Innovative Sampling Methodsmentioning

confidence: 99%

Mass Spectrometry-Based Cellular Metabolomics: Current Approaches, Applications, and Future Directions

et al. 2020

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“…The various liquid extraction techniques include liquid extraction surface analysis (LESA) [8], nanospray desorption electrospray ionisation (nano-DESI) and devices such as the FlowProbe (Prosolia Inc. Indianapolis, IN) and the MasSpec Pen [9]. In the last decade, these techniques have become established for analysis of small molecules, metabolites and lipids directly from biological samples (LESA [10][11][12], nano-DESI [13][14][15], FlowProbe [16], MasSpec Pen [17]), but in situ protein analysis has proven a greater challenge.…”

Section: Liquid Junction Surface Samplingmentioning

confidence: 99%

In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates

Hale

Cooper

2020

Biochemical Society Transactions

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Advances in sample preparation, ion sources and mass spectrometer technology have enabled the detection and characterisation of intact proteins. The challenges associated include an appropriately soft ionisation event, efficient transmission and detection of the often delicate macromolecules. Ambient ion sources, in particular, offer a wealth of strategies for analysis of proteins from solution environments, and directly from biological substrates. The last two decades have seen rapid development in this area. Innovations include liquid extraction surface analysis, desorption electrospray ionisation and nanospray desorption electrospray ionisation. Similarly, developments in native mass spectrometry allow protein–protein and protein–ligand complexes to be ionised and analysed. Identification and characterisation of these large ions involves a suite of hyphenated mass spectrometry techniques, often including the coupling of ion mobility spectrometry and fragmentation techniques. The latter include collision, electron and photon-induced methods, each with their own characteristics and benefits for intact protein identification. In this review, recent developments for in situ protein analysis are explored, with a focus on ion sources and tandem mass spectrometry techniques used for identification.

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Liquid extraction surface analysis nanospray electrospray ionization based lipidomics for in situ analysis of tumor cells with multidrug resistance

Cited by 16 publications

References 33 publications

<p>Metabolomics: a promising diagnostic and therapeutic implement for breast cancer</p>

<p>Metabolomics: a promising diagnostic and therapeutic implement for breast cancer</p>

Mass Spectrometry-Based Cellular Metabolomics: Current Approaches, Applications, and Future Directions

In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates

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