Liquid‐phase‐based separation systems for depletion, prefractionation and enrichment of proteins in biological fluids and matrices for in‐depth proteomics analysis – An update covering the period 2008–2011

Selvaraju, Subhashini; Rassi, Ziad El

doi:10.1002/elps.201100431

Cited by 73 publications

(50 citation statements)

References 98 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Purification of serum proteins remains a technique challenge in the field of proteomics. 35 Combination of Nano Aquity Ultra Performance Liquid Chromatography and LTQ Orbitrap may shed light in this regard.…”

Section: Discussionmentioning

confidence: 99%

Serum protein profiles of patients with lung cancer of different histological types

Yang¹,

Tian

Ren

et al. 2015

Asia-Pac J Clncl Oncology

View full text Add to dashboard Cite

Aims: To compare serum protein expression profiles between lung cancer patients and healthy individuals, and to examine whether there are differences in serum protein expression profiles among patients with lung cancers of different histological types and whether the characteristic expression of serum proteins may assist in differential diagnosis of various subtypes of lung cancers. Methods: Blood samples were collected from 123 lung cancer patients before commencement of treatment who attended Shanxi Cancer Hospital, China, between 2008 and 2013. Blood samples from 60 healthy individuals were also collected in the same period. Serum protein expression profiles were analyzed using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. The differences in the serum protein spectrums of lung cancer patients with different histological subtypes were analyzed by one-way Analysis of Variance and receiver operating characteristic curves. Results: A cluster of 48 protein mass-to-change ratio (M/Z) peaks was differentially expressed between sera of lung cancer patients and healthy individuals. The M/Z 1205, 4673, 1429 and 4279 peaks were differentially expressed among patients with lung squamous cell carcinomas, adenocarcinomas and small-cell lung carcinomas. Conclusion: These results reinforce the notion that profiling of serum proteins may be of diagnostic value in lung cancer, and suggest that the differences in serum protein profiles may be useful in differential diagnosis of lung cancers of varying histological subtypes.

show abstract

Section: Discussionmentioning

confidence: 99%

Serum protein profiles of patients with lung cancer of different histological types

Yang¹,

Tian

Ren

et al. 2015

Asia-Pac J Clncl Oncology

View full text Add to dashboard Cite

show abstract

“…Hence, it is foreseeable that specialized antigen arrays, such as fluid-phase systems, may replace ELISA-based studies for the clinical use of multiplexed antigen assays in clinical laboratories in the future (Selvaraju and El Rassi 2012;Tjalsma et al 2008). Regardless of these challenges, antigen-profiling advances will be of great clinical value for screening, diagnosis, prognosis, and monitoring not only infection but also therapeutic intervention in autoimmune diseases and cancer.…”

Section: Future Perspectivesmentioning

confidence: 99%

Proteome signatures—how are they obtained and what do they teach us?

Costa

Ferreira

Amado

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The dawn of a new Proteomics era, just over a decade ago, allowed for large-scale protein profiling studies that have been applied in the identification of distinctive molecular cell signatures. Proteomics provides a powerful approach for identifying and studying these multiple molecular markers in a vast array of biological systems, whether focusing on basic biological research, diagnosis, therapeutics, or systems biology. This is a continuously expanding field that relies on the combination of different methodologies and current advances, both technological and analytical, which have led to an explosion of protein signatures and biomarker candidates. But how are these biological markers obtained? And, most importantly, what can we learn from them? Herein, we briefly overview the currently available approaches for obtaining relevant information at the proteome level, while noting the current and future roles of both traditional and modern proteomics. Moreover, we provide some considerations on how the development of powerful and robust bioinformatics tools will greatly benefit high-throughput proteomics. Such strategies are of the utmost importance in the rapidly emerging field of immunoproteomics, which may play a key role in the identification of antigens with diagnostic and/or therapeutic potential and in the development of new vaccines. Finally, we consider the present limitations in the discovery of new signatures and biomarkers and speculate on how such hurdles may be overcome, while also offering a prospect for the next few years in what could be one of the most significant strategies in translational medicine research.

show abstract

“…This step also decreases the dynamic concentration range of plasma proteins, enriches the subproteome of interest and provides pre-fractionation before deeper analysis [34]. [35,36].…”

Section: Plasma Fractionation For Proteomicsmentioning

confidence: 99%

Medicinal Chemistry Meets Proteomics: Fractionation of the Human Plasma Proteome

Kovács¹,

Guttman²

2013

CMC

View full text Add to dashboard Cite

Human plasma and its fractions/derivatives are frequently used materials in biomedicine as it contains thousands and thousands of proteins representing the majority of human proteome. Several important methods were developed in the past for the fractionation of this important biological fluid and their use for medicinal purposes. One of the greatest challenges is the very large dynamic range of plasma proteins ranging up to 10-12 orders of magnitude. Early attempts were mainly based on methods such as salting out or cold ethanol precipitation, as well as chromatography utilizing affinity, size exclusion, ion exchange and hydrophobic interaction techniques. More recently, fractionation applications started with the depletion of the high abundant plasma components, such as serum albumin and immunoglobulins, before isolating lower abundant proteins of interest. Plasma volumes were utilized from the milliliter scale for diagnostic applications to hundreds of liters for industrial scale plasma fractionation (e.g., medicinal product manufacturing). In this paper we review this important part of medicinal chemistry, highlighting the traditional methods along with some of their variations as well as the most significant recent achievements of the field.

show abstract

Liquid‐phase‐based separation systems for depletion, prefractionation and enrichment of proteins in biological fluids and matrices for in‐depth proteomics analysis – An update covering the period 2008–2011

Cited by 73 publications

References 98 publications

Serum protein profiles of patients with lung cancer of different histological types

Serum protein profiles of patients with lung cancer of different histological types

Proteome signatures—how are they obtained and what do they teach us?

Medicinal Chemistry Meets Proteomics: Fractionation of the Human Plasma Proteome

Contact Info

Product

Resources

About