List of Contributors

Chlamydia psittaci inhibits the production of reactive oxygen species in human neutrophils by inhibiting NADPH oxidase through the p38/ERK signaling pathway

“…qPCR was performed on DNA extracted from hPMNs infected C. psittaci according to the manufacturer's instructions. Primers used for C. psittaci 16S Rrna(forword:5'-TCCGCAAGGACAGATACACA-3' reverse:5'-ACCCAGGCAGTCTCGTTAGA-3') and human β-actin (forword:5'-GGCATCGTGATGGACTCCG-3' reverse:5'-GCTGGAAGGTGGACAGCGA-3') were as previously described (Chen et al, 2021). Quantitative PCR was performed in a 20 µL reaction volume containing 2 µL DNA, 10 ml of 2× mix, 0.03 mM forward and reverse primers, and 6.8 µL ddH 2 O according to the instructions of the SYBR green I reagent kit (Qiagen, Shanghai, China).…”

“…No infection is a negative control, PMA stimulation is a positive control. C. psittaci of MOI (1,10,20,40) were used to infect hPMNs. As shown in Figure .1B, as the MOI of C. psittaci continues to increase, so does the load of C. psittaci.…”

“…In order to demonstrate the oxidative stress response after C. psittaci infection with hPMNs, ROS production in hPMNs infected and uninfected with C. psittaci was measured using DCFH-DA with and without PMA. After the hPMNs cells were infected with C. psittaci of different MOI (1,10,20,40) for 60 min, the ROS were detected by ow cytometry and statistically analyzed. As shown in Figure .3A,B,C,D, unstimulated hPMNs did not produce detectable ROS.…”