Lithium chloride assuages bone loss in experimental periodontitis in estrogen-deficient rats

Malta, Fernando de Souza; Napimoga, Marcelo Henrique; Marins, Letícia Macedo; Miranda, Tamires Szeremeske; Oliveira, Flavianny Bárbara de; Posch, Aline Tany; Feres, Magda; Duarte, Poliana Mendes

doi:10.1007/s00784-019-03067-9

Cited by 9 publications

(7 citation statements)

References 41 publications

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“…3). In support of these findings, previous investigations also reported that lithium chloride can increase the expression of RANKL (20,29). Nonetheless, the increased expression of RANKL that was observed, herein, during lithium treatment is somewhat surprising as bone healing was improved in the Lithium chloride group even though RANKL is a marker of osteoclastogenesis.…”

Section: Discussionsupporting

confidence: 84%

“…In fact, lithium chloride treatment increased the expression of the three markers of osteogenesis including OCN, a middle stage marker, OPN, a late stage marker and BSP, a marker of osteoblast differentiation (30). Previous studies have already revealed similar trends towards the increased expression of osteogenic/osteoblast markers in the presence of lithium chloride (16,20,(31)(32)(33). Taken together, these histometric and immunohistochemical findings suggest that lithium chloride may represent a promising pharmacological agent for bone repair in estrogen deficient status, particularly in the early to the middle healing stages.…”

Section: Discussionmentioning

confidence: 74%

“…In support of these findings, previous investigations have demonstrated potential benefits of lithium chloride on fracture healing (26,27) and alveolar bone repair (16)(17)(18)(19)(20)(21). Han et al (16) demonstrated that local administration of lithium chloride stimulated greater alveolar bone volume and mineral density during the healing of surgicallycreated periodontal defects in estrogen-sufficient rats.…”

Section: Discussionmentioning

confidence: 76%

“…Pre-clinical dental studies have shown benefits of lithium chloride, in states of both estrogen sufficiency and deficiency, on bone formation during orthodontic retention, midpalatal expansion and surgically-created periodontal defects, for improving implant osseointegration and for the attenuation of bone loss in a model of induced periodontitis (15)(16)(17)(18)(19)(20). A single previous study in estrogen-sufficient rats evaluated the effects of lithium chloride on the healing process of tooth sockets.…”

Section: Introductionmentioning

confidence: 99%

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Systemic Lithium Chloride Administration Improves Tooth Extraction Wound Healing in Estrogen-Deficient Rats

et al. 2020

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The purpose of this investigation was to evaluate the effects of lithium chloride (LiCl) on the socket healing of estrogen-deficient rats. Seventy-two rats were allocated into one of the following groups: Control, Ovariectomy and LiCl (150 mg/kg/2 every other day orally) + Ovariectomy. Animals received LiCl or water from the 14th day post-ovariectomy, until the completion of the experiment. On the 21st day after ovariectomy, the first molars were extracted. Rats were euthanized on the 10th, 20th and 30th days following extractions. Bone healing (BH), TRAP positive cells and immunohistochemical staining for OPG, RANKL, BSP, OPN and OCN were evaluated. The Ovariectomy group presented decreased BH compared to the LiCl group at 10 days, and the lowest BH at 20 days (p<0.05). At 30 days, the Ovariectomy and LiCl-groups presented lower BH than that of the Control (p<0.05). The number of TRAP-stained cells was the lowest in the LiCl group at 20 days and the highest in the Ovariectomy group at 30 days (p<0.05). At 10 days of healing, the LiCl group demonstrated stronger staining for all bone markers when compared to the other groups, while the Ovariectomy group presented higher RANKL expression than that of the Control (p<0.05). LiCl enhanced bone healing in rats with estrogen deficiency, particularly in the initial healing phases. However, as data on the effects of lithium chloride on bone tissue are still preliminary, more studies related to its toxicity and protocol of administration are necessary before its application in clinical practice.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

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Systemic Lithium Chloride Administration Improves Tooth Extraction Wound Healing in Estrogen-Deficient Rats

et al. 2020

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“…Drugs that target more than one gene were considered more credible and effective ( Table 2 ). Some of them, including Methotrexate, Melatonin, Resveratrol, Diacerein, Lithium and Infliximab, have been used to treat periodontitis or T2DM in animal experiments ( 43 – 47 , 49 ), while the remaining drugs (Hydroquinone, Echinacea, Hydrocortisone, Cisplatin, Omeprazole, Ibudilast, Lansoprazole) have not been found to have therapeutic effects in periodontitis with T2DM. Notably, Cisplatin, a metal-based anticancer agent, has been reported to aggravate periodontitis ( 48 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of cross-talk pathways and ferroptosis-related genes in periodontitis and type 2 diabetes mellitus by bioinformatics analysis and experimental validation

Pan

Sun

et al. 2022

Front. Immunol.

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PurposeThere is a bidirectional relationship between periodontitis and type 2 diabetes mellitus (T2DM). The aim of this study was to further explore the pathogenesis of this comorbidity, screen out ferroptosis-related genes involved in the pathological process, and predict potential drug targets to develop new therapeutic strategies.MethodsCommon cross-talk genes were identified from periodontitis datasets (GSE16134, GSE10334 and GSE106090) and T2DM databases (DisGeNET and GeneCard). Then, GO and KEGG enrichment analyses, PPI network analysis and hub gene identification were performed. The association between ferroptosis and periodontitis with T2DM was investigated by Pearson correlation analysis. Core ferroptosis-related cross-talk genes were identified and verified by qRT-PCR. Potential drugs targeting these core genes were predicted via DGIDB.ResultsIn total, 67 cross-talk genes and two main signalling pathways (immuno-inflammatory pathway and AGE-RAGE signalling pathway) were identified. Pearson correlation analysis indicated that ferroptosis served as a crucial target in the pathological mechanism and treatment of periodontitis with T2DM. IL-1β, IL-6, NFE2L2 and ALOX5 were identified as core ferroptosis-related genes and the qRT-PCR detection results were statistically different. In total, 13 potential drugs were screened out, among which, Echinacea and Ibudilast should be developed first.ConclusionsThis study contributes to a deeper understanding of the common pathogenesis of periodontitis and T2DM and provides new insights into the role of ferroptosis in this comorbidity. In addition, two drugs with potential clinical application value were identified. The potential utility of these drugs requires further experimental investigation.

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Lithium chloride stimulates bone formation in extraction socket repair in rats

Duarte

Miranda²,

Marins³

et al. 2022

Oral Maxillofac Surg

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Lithium chloride assuages bone loss in experimental periodontitis in estrogen-deficient rats

Cited by 9 publications

References 41 publications

Systemic Lithium Chloride Administration Improves Tooth Extraction Wound Healing in Estrogen-Deficient Rats

Systemic Lithium Chloride Administration Improves Tooth Extraction Wound Healing in Estrogen-Deficient Rats

Identification of cross-talk pathways and ferroptosis-related genes in periodontitis and type 2 diabetes mellitus by bioinformatics analysis and experimental validation

Lithium chloride stimulates bone formation in extraction socket repair in rats

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