Lithium-containing biomaterials stimulate bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis

Liu, Lu; Liu, Yaqin; Feng, Chun; Chang, Jiang; Fu, Runqing; Wu, Tingting; Yu, Fei; Wang, Xiaoting; Xia, Lunguo; Wu, Chengtie; Fang, Bing

doi:10.1016/j.biomaterials.2018.11.007

Cited by 136 publications

(105 citation statements)

References 49 publications

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“…The exosome is a recently discovered extracellular vesicle that is important in intercellular communications [25]. Recent studies have reported that drugs, hormones and environmental factors could alter exosome contents and intercellular communication [26]. Therefore, we attributed in vivo re-epithelization promotion by PTHrP-2 to exosome-mediated changes in intercellular communication.…”

Section: Introductionmentioning

confidence: 90%

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities

et al. 2020

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Background: Diabetic wounds are a disturbing and rapidly growing clinical problem. A novel peptide, parathyroid hormone related peptide (PTHrP-2), is assumed as multifunctional factor in angiogenesis, fibrogenesis and reepithelization. This study aims to test PTHrP-2 efficiency and mechanism in wound healing. Methods: Through repair phenomenon in vivo some problems were detected, and further research on their mechanisms was made. In vivo therapeutic effects of PTHrP-2 were determined by HE, Masson, microfil and immunohistochemical staining. In vitro direct effects of PTHrP-2 were determined by proliferation, migration, Vascular Endothelial Grown Factor and collagen I secretion of cells and Akt/ Erk1/2 pathway change. In vitro indirect effects of PTHrP-2 was study via exosomes. Exosomes from PTHrP-2 untreated and treated HUVECs and HFF-1 cells were insolated and identified. Exosomes were co-cultured with original cells, HUVECs or HFF-1 cells, and epithelial cells. Proliferation and migration and pathway change were observed. PTHrP-2-HUVEC-Exos were added into in vivo wound to testify its hub role in PTHrP-2 indirect effects in wound healing. Results: In vivo, PTHrP-2 exerted multifunctional pro-angiogenesis, pro-firbogenesis and re-epithelization effects. In vitro, PTHrP-2 promoted proliferation and migration of endothelial and fibroblast cells, but had no effect on epithelial cells. Therefore, we tested PTHrP-2 indirect effects via exosomes. PTHrP-2 intensified intercellular communication between endothelial cells and fibroblasts and initiated endothelial-epithelial intercellular communication. PTHrP-2-HUVEC-Exos played a hub role in PTHrP-2 indirect effects in wound healing.Conclusion: These findings of this study indicated that PTHrP-2, a multifunctional factor, could promote wound healing via synergistic multicellular stimulating and exosomal activities.

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Section: Introductionmentioning

confidence: 90%

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities

et al. 2020

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“…Similarly, it has been proposed by Yang et al that exosome-derived miR-130a activated angiogenesis in gastric cancer in vascular endothelial cells, 31 and a recent study has revealed that lithium-containing biomaterials stimulated bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis. 32 Moreover, miR-130a has also been unravelled to increase osteogenic differentiation and attenuate adipogenic differentiation of bone marrow mesenchymal stem cells. 33 In addition, Li et al have found that miR-130a inhibition protected rat cardiac myocytes from hypoxia-triggered apoptosis, 34 and it has been uncovered that miR-130a alleviated neuronal apoptosis and changes in expression of Bcl-2/Bax and Caspase-3 in cerebral infarction rats.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‐130a regulates neurological deficit and angiogenesis in rats with ischaemic stroke by targeting XIAP

Deng

Fan

Mao

et al. 2020

J Cellular Molecular Medi

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MicroRNAs (miRNAs) have already been proposed to be implicated in the development of ischaemic stroke. We aim to investigate the role of miR‐130a in the neurological deficit and angiogenesis in rats with ischaemic stroke by regulating X‐linked inhibitor of apoptosis protein (XIAP). Middle cerebral artery occlusion (MCAO) models were established by suture‐occluded method, and MCAO rats were then treated with miR‐130a mimics/inhibitors or/and altered XIAP for detection of changes of rats’ neurological function, nerve damage and angiogenesis in MCAO rats. The oxygen‐glucose deprivation (OGD) cellular models were established and respectively treated to determine the roles of miR‐130a and XIAP in neuronal viability and apoptosis. The expression levels of miR‐130a and XIAP in brain tissues of MCAO rats and OGD‐treated neurons were detected. The binding site between miR‐130a and XIAP was verified by luciferase activity assay. MiR‐130a was overexpressed while XIAP was down‐regulated in MCAO rats and OGD‐treated neurons. In animal models, suppressed miR‐130a improved neurological function, alleviated nerve damage and increased new vessels in brain tissues of rats with MCAO. In cellular models, miR‐130a inhibition promoted neuronal viability and suppressed apoptosis. Inhibited XIAP reversed the effect of inhibited miR‐130a in both MCAO rats and OGD‐treated neurons. XIAP was identified as a target of miR‐130a. Our study reveals that miR‐130a regulates neurological deficit and angiogenesis in rats with MCAO by targeting XIAP.

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“…The exosome is a recently discovered extracellular vesicle that is important in intercellular communications (24). Recent studies have reported that drugs, hormones and environmental factors can alter exosome contents and intercellular communication (25). Therefore, we attribute in vivo reepithelization promotion by PTHrP-2 to exosome-mediated changes in intercellular communication.…”

mentioning

confidence: 92%

PTH Derivative Promotes Chronic Wound Healing via Synergistic Multicellular Stimulating and Exosomal Activities

Shen

Huang

Wang

et al. 2019

Preprint

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Background: Chronic diabetic wounds are a disturbing and rapidly growing clinical problem. Parathyroid hormone related peptide (PTHrP-2) was assumed as multifunctional factor in angiogenesis, fibrogenesis and re-epithelization. This study aims to test PTHrP-2 efficiency and mechanism in chronic wound healing. Methods: Through repair phenomenon in vivo some problems were detected, and further research on their mechanisms was made. In vivo therapeutic effects of PTHrP-2 was determined by HE, Masson, microfil and immunohistochemical staining. In vitro direct effects of PTHrP-2 was determined by proliferation, migration, Vascular Endothelial Grown Factor and collagen I secretion of cells and Akt/ Erk1/2 pathway change. In vitro indirect effects of PTHrP-2 was study via exosomes. Exosomes from PTHrP-2 untreated and treated HUVECs and HFF-1 cells were insolated and identified. Exosomes were co-cultured with original cells, HUVECs or HFF-1 cells, and epithelial cells. Proliferation and migration and pathway change were observed. PTHrP-2-HUVEC-Exos was added into in vivo wound to testify its hub role in PTHrP-2 indirect effects in wound healing. Results: In vivo, PTHrP-2 exerted multifunctional pro-angiogenesis, pro-firbogenesis and re-epithelization effects. In vitro, PTHrP-2 promoted proliferation and migration of endothelial and fibroblast cells, but had no effect on epithelial cells. Therefore, we tested PTHrP-2 indirect effects via exosomes. PTHrP-2 intensified intercellular communication between endothelial cells and fibroblasts and initiated endothelial-epithelial intercellular communication. PTHrP-2-HUVEC-Exos played hub role in PTHrP-2 indirect effects in wound healing. Conclusion: The findings of this study indicate that PTHrP-2, a multifunctional factor, can promote chronic wound healing via synergistic multicellular stimulating and exosomal activities.

show abstract

Lithium-containing biomaterials stimulate bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis

Cited by 136 publications

References 49 publications

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities

PTH Derivative promotes wound healing via synergistic multicellular stimulating and exosomal activities

MicroRNA‐130a regulates neurological deficit and angiogenesis in rats with ischaemic stroke by targeting XIAP

PTH Derivative Promotes Chronic Wound Healing via Synergistic Multicellular Stimulating and Exosomal Activities

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