Lithium doped calcium phosphate cement maintains physical mechanical properties and promotes osteoblast proliferation and differentiation

Abstract

Calcium phosphate cement (CPC) has been widely used in bone tissue repairing due to its physical mechanical properties and biocompatibility. Addition of trace element to CPC has shown promising evidence to improve the physical properties and biological activities of CPC. Lithium (Li) has effect on osteoblast proliferation and differentiation. In this study, we incorporated Li to CPC and examined the physical properties of Li/CPC and its effect on osteoblast proliferation and differentiation. We found that Li doped CPC maintained similar setting time, pore size distribution, compressive strength, composition, and morphology as CPC without Li. Additionally, Li doped CPC improved osteoblast proliferation and differentiation significantly compared to CPC without Li. To our knowledge, our results, for the first time, show that Li doped CPC has beneficial effect on osteoblast in cell culture while keeps the excellent physical-mechanical properties of CPC. This study will lead to potential application of Li doped CPC in bone tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 944-952, 2017.

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“…2B). Combined with the results shown above, which consistently reflected HA formation, we concluded that Li/CPC had favourable bioactivity in vitro to the same extent as that of CPC, a finding that is in accordance with other reports23.…”

“…Li/CPC was prepared according to a method described previously23. TTCP (Sigma–Aldrich, Saint Louis, MO, USA) and DCPA (Sigma–Aldrich) were mixed at a ratio of 1:1 as a powder phase.…”

“…Liquid extracts were prepared by immersion of cements in Dulbecco’s modified Eagle’s medium-alpha (DMEM-α; Hyclone, San Angelo, TX, USA) containing 10% FBS (Corning, Corning, NY, USA) and antibiotics (10 U/ml penicillin, 100 mg/ml streptomycin; Sigma–Aldrich) (1.25 cm 2 /ml, 37 °C, 24 h) according to International Standard Organization 10993-5 and the literature23. Differentiated solutions were prepared by supplementation with 100 nM dexamethasone (Sigma–Aldrich), 10 mM β-glycerophosphate (Sigma–Aldrich) and 0.2 mM ascorbic acid (Sigma–Aldrich) in different extracts.…”

“…Pilot studies by our research team have shown that lithium-doped calcium phosphate cement (Li/CPC) can promote the proliferation and differentiation of osteoblasts in vivo , and enhance osteogenesis in defects in rats compared with CPC222324. In the present study, we explored the underlying mechanism of the proliferation and differentiation of cells under stimulation by lithium released from Li/CPC, and observed the role of Li/CPC in the repair of osteoporotic bone defects.…”

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“…Using human osteosarcoma (MG63) cells, several studies in recent years also reported that lithium had promising effects on osteoblast proliferation and differentiation. Lithium-doped CPC had high biocompatibility, supported osteoblast attachment and growth, and promoted cell proliferation and differentiation in MG-63 cells (Li et al, 2017b). It was noteworthy that MG-63 cells cultured on bone substitute coated with lithium had higher cell viability, cell proliferation, cell differentiation, calcium deposition, and expression of osteogenesis-related genes (Guo et al, 2018;Liu et al, 2018;Ma et al, 2019).…”