Lithium impacts the function of hematopoietic stem cells via disturbing the endoplasmic reticulum stress and Hsp90 signaling

Liu, Yalin; Zhao, Yifan; Wu, Jiaojiao; Liu, Ting; Tang, MengKe; Yao, Ye; Xue, Peng; He, Miao; Xu, Yanyi; Zhang, Peng; Gu, Minghua; Qu, Weidong; Zhang, Yubin

doi:10.1016/j.fct.2023.114081

Cited by 4 publications

(2 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the canonical Wnt signaling drives the proliferation of HSCs, and the non-canonical Wnt signaling promotes myelopoiesis while suppressing lymphopoiesis in HSCs (Florian et al, 2012;López-Ruano et al, 2015;Zhao et al, 2018;Zhao et al, 2023). A direct action and/or an indirect action of a metal may impact certain signaling pathways to alter the behavior of HSCs (Liu et al, 2023;Zhao et al, 2018;Zhao et al, 2023). Thus, investigating the signaling pathways responsible for alteration of HSCs behavior is a pivotal aspect for elucidating the mechanisms of the immune and hematopoietic toxicology of metals.…”

Section: Screen the Potential Signaling Molecules In Hscs During Meta...mentioning

confidence: 99%

“…Metals, such as lead (Pb), cadmium (Cd), and lithium (Li), broadly exist in the environment and are toxic to the immune and hematopoietic systems (Bjorklund et al, 2020;Liu et al, 2023;Mirkov et al, 2021). Hematopoietic stem cells (HSCs), the progenitor of all blood cells, are able to self-renew and differentiate into all types of immune cells, as well as erythrocytes and platelets (Metcalf, 2007).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ex Vivo Evaluation of the Function of Hematopoietic Stem Cells in Toxicology of Metals

Wu,

Liu,

Tang

et al. 2024

Current Protocols

Self Cite

View full text Add to dashboard Cite

A variety of metals, e.g., lead (Pb), cadmium (Cd), and lithium (Li), are in the environment and are toxic to humans. Hematopoietic stem cells (HSCs) reside at the apex of hematopoiesis and are capable of generating all kinds of blood cells and self‐renew to maintain the HSC pool. HSCs are sensitive to environmental stimuli. Metals may influence the function of HSCs by directly acting on HSCs or indirectly by affecting the surrounding microenvironment for HSCs in the bone marrow (BM) or niche, including cellular and extracellular components. Investigating the impact of direct and/or indirect actions of metals on HSCs contributes to the understanding of immunological and hematopoietic toxicology of metals. Treatment of HSCs with metals ex vivo, and the ensuing HSC transplantation assays, are useful for evaluating the impacts of the direct actions of metals on the function of HSCs. Investigating the mechanisms involved, given the rarity of HSCs, methods that require large numbers of cells are not suitable for signal screening; however, flow cytometry is a useful tool for signal screening HSCs. After targeting signaling pathways, interventions ex vivo and HSCs transplantation are required to confirm the roles of the signaling pathways in regulating the function of HSCs exposed to metals. Here, we describe protocols to evaluate the mechanisms of direct and indirect action of metals on HSCs. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Identify the impact of a metal on the competence of HSCsBasic Protocol 2: Identify the impact of a metal on the lineage bias of HSC differentiationBasic Protocol 3: Screen the potential signaling molecules in HSCs during metal exposureAlternate Protocol 1: Ex vivo treatment with a metal on purified HSCsAlternate Protocol 2: Ex vivo intervention of the signaling pathway regulating the function of HSCs during metal exposure

show abstract

Section: Screen the Potential Signaling Molecules In Hscs During Meta...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ex Vivo Evaluation of the Function of Hematopoietic Stem Cells in Toxicology of Metals

Wu,

Liu,

Tang

et al. 2024

Current Protocols

Self Cite

View full text Add to dashboard Cite

show abstract

Impact of Lithium on the Immune System: An Investigation of T-Cell Subpopulations and Cytokine Responses in Rats

Matur,

Akyol,

Toplan

et al. 2024

Biol Trace Elem Res

View full text Add to dashboard Cite

The aim of this study was to investigate the dose-dependent adverse effects of long-term dietary lithium administration on specific aspects of the defense system in rats. Additionally, the study aimed to explore the inflammatory activities of lithium beyond its recognized anti-inflammatory properties. Forty Wistar Albino rats were involved, which were randomly allocated into the control and four treatment groups. The control group received standard rat feed, and the experimental groups’ diet was added 1 g/kg, 1.4 g/kg, 1.8 g/kg, and 2.2 g/kg lithium bicarbonate, respectively. CD4+, CD8+, and CD161 + cells were assessed by flow cytometry. TNF-α, IFN-γ, IL-1β, and IL-2 and IL-4, IL-6, and IL-10 levels were measured. The proportion of CD4 + cells and the CD4+/CD8 + ratio (P = 0.005 and P = 0.038, respectively) were reduced with the highest dose of lithium compared to the control group. The data regarding pro-inflammatory cytokines showed a dose-dependent increase in serum TNF-α and IFN-γ levels (P = 0.023 and P = 0.001, respectively). On the other hand, serum IL-1β and IL-2 levels were decreased in a dose-dependent manner (P = 0. 001 and P = 0. 001, respectively). As for anti-inflammatory cytokines, a dose-dependent decrease was determined in serum IL-4 level (P = 0.002), while no significant changes were noted in IL-6 and IL-10 levels (P = 0.507 and P = 0.732, respectively). In conclusion, lithium adversely impacted the cellular defense system. Furthermore, apart from its anti-inflammatory properties, lithium exhibited cytokine-mediated inflammatory activities. Therefore, lithium’s potential adverse effects on the immune system should be considered in immunodeficient patients and those with an inflammatory status treated with high doses of lithium.

show abstract

Importance of salinity on regulating the environmental fate and bioaccumulation of lithium in the Yangtze River Estuary

Zou,

Wang,

Yang

et al. 2024

Science of The Total Environment

View full text Add to dashboard Cite

Lithium impacts the function of hematopoietic stem cells via disturbing the endoplasmic reticulum stress and Hsp90 signaling

Cited by 4 publications

References 49 publications

Ex Vivo Evaluation of the Function of Hematopoietic Stem Cells in Toxicology of Metals

Ex Vivo Evaluation of the Function of Hematopoietic Stem Cells in Toxicology of Metals

Impact of Lithium on the Immune System: An Investigation of T-Cell Subpopulations and Cytokine Responses in Rats

Importance of salinity on regulating the environmental fate and bioaccumulation of lithium in the Yangtze River Estuary

Contact Info

Product

Resources

About