Lithography-based methods to manufacture biomaterials at small scales

Tran, Khanh T. M.; Nguyen, Thanh D.

doi:10.1016/j.jsamd.2016.12.001

Cited by 96 publications

(78 citation statements)

References 178 publications

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“…In neural tissue engineering, electrical stimuli together with a controlled topography (e.g., aligned nanofibers) have been identified as key parameters to promote nerve regeneration and neural stem cells differentiation; in particular, nerve guidance channels are indicated to induce a forced organization of the cells that can lead to a cytoskeletal modification according to the channels direction [31][32][33]. Photolithography has been used for the production of scaffolds and the manufacturing of biomaterials at small scales for tissue engineering applications [34][35][36]. On the other hand, the pyrolysis technique has been considered less frequently and specifically for neural tissue engineering [37,38].…”

Section: Introductionmentioning

confidence: 99%

Production of carbonized micro-patterns by photolithography and pyrolysis

Ginestra

Madou

Ceretti

2019

Precision Engineering

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The preparation of carbon micro-patterns is reported in this paper. Different carbon micro-patterns were created using photolithography of the epoxy-based negative photoresist SU-8. Photoresist patterns were optimized in terms of resolution and aspect ratio and subsequently subjected to pyrolysis to obtain carbonized and conductive 3D structures. The latter step requires the optimization of the resist cross-linking time as well as the temperature and time of the resist post-bake. This step is crucial in order to avoid any severe modification of the geometry of the patterns produced during the actual pyrolysis. By observing optical and scanning electron microscope images, the morphology of the structures before and after pyrolysis was studied and the same patterns were also characterized by a laser probe profilometer. Finally, the thus obtained carbon patterns on Si wafers were used to carry out cell culture tests with Neural Stem Cells (NSC). The adhesion and the arrangement of the stem cells were analyzed to verify the ability of the patterned substrates to guide the orientation and, therefore, the differentiation of the cells.

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Section: Introductionmentioning

confidence: 99%

Production of carbonized micro-patterns by photolithography and pyrolysis

Ginestra

Madou

Ceretti

2019

Precision Engineering

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“…Xu and Siedlecki [19] used photolithography and soft lithography to modify polyurethane urea to evaluate bacterial adhesion of S. epidermidis and S. aureus under dynamic conditions and concluded that bacterial adhesion followed a decreasing tendency as the shear rate increased on the modified surface compared to a flat surface, particularly for S. epidermidis in PBS, and adhesion reduction reached values up to 90%. However, photolithography presents several disadvantages, especially in the biomedical field, since it requires expensive equipment and facilities, is composed of several rigorous steps, no control over surface chemistry exists, and cannot be implemented on curved or nonplanar surfaces [20].…”

Section: Introductionmentioning

confidence: 99%

Surface Modification by Combination of Dip-Pen Nanolithography and Soft Lithography for Reduction of Bacterial Adhesion

Arango-Santander

Peláez-Vargas

Freitas

et al. 2018

Journal of Nanotechnology

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Dip-pen nanolithography (DPN) and soft lithography are techniques suitable to modify the surface of biomaterials. Modified surfaces might play a role in modulating cells and reducing bacterial adhesion and biofilm formation. The main objective of this study was threefold: first, to create patterns at microscale on model surfaces using DPN; second, to duplicate and transfer these patterns to a real biomaterial surface using a microstamping technique; and finally, to assess bacterial adhesion to these developed patterned surfaces using the cariogenic species Streptococcus mutans. DPN was used with a polymeric adhesive to create dot patterns on model surfaces. Elastomeric polydimethylsiloxane was used to duplicate the patterns and silica sol to transfer them to the medical grade stainless steel 316L surface by microstamping. Optical microscopy and atomic force microscopy (AFM) were used to characterize the patterns. S. mutans adhesion was assessed by colony-forming units (CFUs), MTT viability assay, and scanning electron microscopy (SEM). DPN allowed creating microarrays from 1 to 5 µm in diameter on model surfaces that were successfully transferred to the stainless steel 316L surface via microstamping. A significant reduction up to one order of magnitude in bacterial adhesion to micropatterned surfaces was observed. The presented experimental approach may be used to create patterns at microscale on a surface and transfer them to other surfaces of interest. A reduction in bacterial adhesion to patterned surfaces might have a major impact since adhesion is a key step in biofilm formation and development of biomaterial-related infections.

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“…Lithography enables the precise fabrication of 2D and 3D structures on small-scale moulds similar to planographic printing (printing from a flat surface, either on a plate or stone) [ 128 , 129 ]. There are advanced lithography-based methods that include photolithography, moulding technology and particle replication in non-wetting template (PRINT) with the potential to develop biotherapeutic formulations.…”

Section: General Strategies To Increase Duration Of Actionmentioning

confidence: 99%

“…Photolithography involves the use of light to transfer patterns onto a substrate. PRINT is a highly versatile method with an elastomeric mould containing wells and cavities that can be used to encapsulate oligonucleotides, polypeptides and synthetic viruses to yield an array of sizes, shapes, compositions and surface properties [ 128 , 130 , 131 ]. Galloway et al [ 132 ] developed a PLGA-based trivalent vaccine against influenza using PRINT technology and compared the generated immune response to soluble antigen.…”

Section: General Strategies To Increase Duration Of Actionmentioning

confidence: 99%

Injectables and Depots to Prolong Drug Action of Proteins and Peptides

et al. 2020

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Proteins and peptides have emerged in recent years to treat a wide range of multifaceted diseases such as cancer, diabetes and inflammation. The emergence of polypeptides has yielded advancements in the fields of biopharmaceutical production and formulation. Polypeptides often display poor pharmacokinetics, limited permeability across biological barriers, suboptimal biodistribution, and some proclivity for immunogenicity. Frequent administration of polypeptides is generally required to maintain adequate therapeutic levels, which can limit efficacy and compliance while increasing adverse reactions. Many strategies to increase the duration of action of therapeutic polypeptides have been described with many clinical products having been developed. This review describes approaches to optimise polypeptide delivery organised by the commonly used routes of administration. Future innovations in formulation may hold the key to the continued successful development of proteins and peptides with optimal clinical properties.

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Lithography-based methods to manufacture biomaterials at small scales

Cited by 96 publications

References 178 publications

Production of carbonized micro-patterns by photolithography and pyrolysis

Production of carbonized micro-patterns by photolithography and pyrolysis

Surface Modification by Combination of Dip-Pen Nanolithography and Soft Lithography for Reduction of Bacterial Adhesion

Injectables and Depots to Prolong Drug Action of Proteins and Peptides

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