Live cell extraction and HPLC–MS analysis for predicting bioactive components of traditional Chinese medicines

Li, Songlin; Li, Ping; Sheng, Liang-Hong; Li, Rui-Yan; Qi, Lian‐Wen; Zhang, Luyong

doi:10.1016/j.jpba.2006.01.014

Cited by 66 publications

(23 citation statements)

References 15 publications

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“…Moreover, we have used a new approach combining biomembrane extraction and high performance liquid chromatography to identify ferulic acid (FA), ligustilide, senkyunolide H, and senkyunolide I, which are some potentially bioavailable components in Angelica sinensis [Dong et al, 2005]. Other studies have identified compounds such as 3-butylphthalide, ligustilide and FA as potential bioactive components in Angelica sinensis using bio-extraction chromatography and immobilized liposome chromatography [Mao et al, 2002;Li et al, 2006].…”

mentioning

confidence: 99%

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Lam

Lin

Lao

et al. 2007

J of Cellular Biochemistry

110

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Angiogenesis plays an important role in a wide range of physiological processes such as wound healing and fetal development. Many diseases are associated with imbalances in regulation of angiogenesis, in which it is either excessive or there is insufficient blood vessel formation. Angelica sinensis (AS), commonly used in the prescriptions of Chinese medicine, is a potential candidate for curing such diseases. However, biological effects of AS on angiogenesis and underlying mechanisms are yet to be fully elucidated. This investigation describes the angiogenic effects of AS extract on human endothelial cells (HUVEC) in vitro and zebrafish in vivo. The extract was demonstrated, by XTT assay and microscopic cell counting, to stimulate the proliferation of HUVEC; in addition, flow cytometry analysis indicated that the extract increased the percentage of HUVEC in the S phase. The wound healing migration assay illustrated that a dramatic increase in migration could be measured in AS extract-treated HUVEC. Meanwhile, the number of invaded cells and the mean tube length were significantly increased in AS extract treatment groups. The extract was also demonstrated to promote changes in subintestinal vessels (SIVs) in zebrafish, one feature of angiogenesis. In addition, AS extract was found by real-time PCR to enhance vascular endothelial growth factor (VEGF) mRNA expression. In a bead-based immunoassay, higher levels of p38 and JNK 1/2 expression were also observed in effusions compared with control cells. All results suggest that Angelica sinensis extract can promote angiogenesis, and that the angiogenic effects involve p38 and JNK 1/2 phosphorylation.

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mentioning

confidence: 99%

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Lam

Lin

Lao

et al. 2007

J of Cellular Biochemistry

110

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“…were proposed for screening and analysis of the multiple bioactive compounds in TCMs by the groups of Zou and Li [4,9,[17][18][19][20][21][22][23][24][25][26]. HPLC or HPLC-MS is the commonly used analytical means, but according to the different sampling methods, these biofingerprint analyses are classified into three types, microdialysis, equilibrium dialysis and cell or target molecule extraction methods.…”

Section: Screening the Bioactive Markers By Interacting Tcms With Tarmentioning

confidence: 99%

“…Cell or target molecule extraction is based on the hypothesis that the potential bioactive compounds in the TCMs could selectively combine with the target cells or some target molecules, so the relative concentrations of the cell-combining components in the suspension medium should decrease and the cell-combining components would be detectable in the extract of denatured cells [22]. Based on the proposed methods, the potential active components of A. sinensis, Cordyceps sinensis, combined formulations of DBD, Shaofu Zhuyu decoction, etc.…”

Section: Screening the Bioactive Markers By Interacting Tcms With Tarmentioning

confidence: 99%

Recent analytical approaches in quality control of traditional Chinese medicines—A review

Jiang

David

et al. 2010

Analytica Chimica Acta

429

268

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“…This method was based on the hypothesis that, when cells are incubated with the extraction of the TCM, the potential bioactive compounds should selectively combined with the cell membrane or be transported into the cell system. Thereby, the relative concentrations of cell-combining components in the suspension medium will decrease and these components will be detected in the extract of denatured cells (Li et al, 2006;Yu et al, 2007). Li et al predicted the bioactive components of TCM by live cell extract and HPLC-MS (Li et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Thereby, the relative concentrations of cell-combining components in the suspension medium will decrease and these components will be detected in the extract of denatured cells (Li et al, 2006;Yu et al, 2007). Li et al predicted the bioactive components of TCM by live cell extract and HPLC-MS (Li et al, 2006). Dong et al proposed an approach for screening and analyzing bioactive components in TCM using red cell membrane extraction and HPLC-MS analysis (Dong et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Screening and analyzing the potential bioactive components from Shaofu Zhuyu decoction, using human umbilical vein endothelial cell extraction and high‐performance liquid chromatography coupled with mass spectrometry

Hua

et al. 2008

Biomedical Chromatography

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In this paper, a useful method for screening and analyzing the potential bioactive components in bioassay-guided fraction (SF-11) from Shaofu Zhuyu decoction was developed using human umbilical vein endothelial cell (HUVEC) extraction and high-performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on HUVEC damage induced by adrenaline in vitro were also investigated. The results showed that SF-11 significantly inhibited the endothelin (ET) release and reversed the NO secretion of HUVEC (p < 0.05), and promoted the PGI(2) release of HUVEC (p < 0.05). Two effective components, paeoniflorin and typhaneoside, from SF-11 were screened and identified using live cell extract and HPLC coupled with Q-TOF/MS spectrometry. The compounds, paeoniflorin and typhaneoside, showed significantly inhibiting effects on the ET release and reversing of NO secretion of HUVEC (p < 0.05), with similar effects to SF-11, and promoting the PGI(2) release of HUVEC at the concentration of 0.208 and 0.013 micromol/mL, respectively (p < 0.05). These data indicated that the method of live cell extraction coupled with HPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCMs.

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Live cell extraction and HPLC–MS analysis for predicting bioactive components of traditional Chinese medicines

Cited by 66 publications

References 15 publications

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

Recent analytical approaches in quality control of traditional Chinese medicines—A review

Screening and analyzing the potential bioactive components from Shaofu Zhuyu decoction, using human umbilical vein endothelial cell extraction and high‐performance liquid chromatography coupled with mass spectrometry

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