Live Lymphocyte Arrays for Biosensing

Kim, Heejae; Cohen, Robert E.; Hammond, Paula T.; Irvine, Darrell J.

doi:10.1002/adfm.200500888

Cited by 64 publications

(63 citation statements)

References 67 publications

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“…By varying the size of microwells, we can generate single cell arrays as well as arrays accepting multicellular clusters composed of different number of cells. This type of lymphocyte-laden microwells has been successfully used for single cell based assays [18][19][20], but has not been used for the study of multicellular interactions. The effect of cluster size can be investigated by comparing cells in different sizes of 'social' microwells, where the degree of multicelluarity is controlled by the dimensions of the microwells.…”

Section: Resultsmentioning

confidence: 99%

“…1 [18]. Firstly, glass coverslips attached at the bottom of the MatTek dishes were functionalized with biotin and acrylate by the scheme shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…1.5 glass window) in a similar way as described elsewhere (Fig. 1) [18]. Firstly, glass surfaces were functionalized with biotin and acrylate groups as schematically shown in Fig.…”

Section: T Cell-laden Hydrogel Microwell Fabricationmentioning

confidence: 99%

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Cell-laden microwells for the study of multicellularity in lymphocyte fate decisions

Doh¹,

Kim²,

Krummel³

2010

Biomaterials

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Section: Resultsmentioning

confidence: 99%

“…1 [18]. Firstly, glass coverslips attached at the bottom of the MatTek dishes were functionalized with biotin and acrylate by the scheme shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Cell-laden microwells for the study of multicellularity in lymphocyte fate decisions

Doh¹,

Kim²,

Krummel³

2010

Biomaterials

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“…[15][16][17][18] The use of sophisticated threedimensional (3D) hydrogel structures would enable important advances in drug delivery, tissue engineering, and favorable material-device interaction. [19][20][21][22][23] For example, 2D cell cultures do not properly imitate the microambiance of threedimensionally-organized native tissue.…”

mentioning

confidence: 99%

Three-Dimensionally-Patterned Submicrometer-Scale Hydrogel/Air Networks That Offer a New Platform for Biomedical Applications

et al. 2008

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Phase mask interference lithography was employed to fabricate three-dimensional (3D) hydrogel structures with high surface area on neural prosthetic devices. A random terpolymer of poly(hydroxyethyl methacrylate-ran-methyl methacrylate-ran-methacrylic acid) was synthesized and used as a negative-tone photoresist to generate bicontinuous 3D hydrogel structures at the submicrometer scale. We demonstrated that the fully open 3D hydrogel/air networks can be used as a pH-responsive polymeric drug-release system for the delivery of neurotrophins to enhance the performance of neural prosthetic devices. Additionally an open hydrogel structure will provide direct access of neuronal growth to the device for improved electrical coupling.

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“…[90][91][92][93] For example, T-cell and B-cell interactions and the mechanisms of signaling between the two types of cells have been studied by locating cells in a microarray. 94 Rapid, sensitive detection of cell-cell signaling, peptide fragments, and pathogens has been performed on the antibody-functionalized surface of microwells. 95 In particular, localizing a number of single mouse ES cells in specific locations by patterning in the microarray has been shown to modulate cell-cell signaling.…”

Section: Microwell-based Analysis Systemmentioning

confidence: 99%

Technology Advancement for Integrative Stem Cell Analyses

Jeong

Choi

Lee

2014

Tissue Engineering Part B: Reviews

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Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose-by introducing a concept of vertical and horizontal approach-that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment.

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Live Lymphocyte Arrays for Biosensing

Cited by 64 publications

References 67 publications

Cell-laden microwells for the study of multicellularity in lymphocyte fate decisions

Cell-laden microwells for the study of multicellularity in lymphocyte fate decisions

Three-Dimensionally-Patterned Submicrometer-Scale Hydrogel/Air Networks That Offer a New Platform for Biomedical Applications

Technology Advancement for Integrative Stem Cell Analyses

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