Liver exhibits thermal variations according to the stage of fibrosis progression: A novel use of modulated‐differential scanning calorimetry for research in hepatology

Escobedo, Galileo; Arjona‐Román, José Luis; Meléndez‐Pérez, Rosalía; Suárez-Álvarez, Karina; Guzmán, Carolina; Aguirre-Garcı́a, Jesús; Gutiérrez-Reyes, Gabriela; Vivas, Oscar; Varela-Fascinetto, Gustavo; Rodrı́guez-Romero, Adela; Robles-Dı́az, Guillermo

doi:10.1111/hepr.12026

Cited by 8 publications

(7 citation statements)

References 43 publications

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“…The thermograms showed an increase of 9.25 • C in the temperature of denaturation of b-lactoglobulin attributed to the binding of vitamin D3, which increased the resistance and stability of the protein. Escobedo et al [44] presented a novel methodology employing MDSC for the detection and quantification of liver fibrosis. Liver fibrosis was induced on male Wistar rats by the use of two experimental procedures including common bile duct ligation (BDL) and carbon tetrachloride administration (CCl4).…”

Section: Food Productsmentioning

confidence: 99%

Application of Differential Scanning Calorimetry (DSC) and Modulated Differential Scanning Calorimetry (MDSC) in Food and Drug Industries

et al. 2019

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Phase transition issues in the field of foods and drugs have significantly influenced these industries and consequently attracted the attention of scientists and engineers. The study of thermodynamic parameters such as the glass transition temperature (Tg), melting temperature (Tm), crystallization temperature (Tc), enthalpy (H), and heat capacity (Cp) may provide important information that can be used in the development of new products and improvement of those already in the market. The techniques most commonly employed for characterizing phase transitions are thermogravimetric analysis (TGA), dynamic mechanical analysis (DMA), thermomechanical analysis (TMA), and differential scanning calorimetry (DSC). Among these techniques, DSC is preferred because it allows the detection of transitions in a wide range of temperatures (−90 to 550 °C) and ease in the quantitative and qualitative analysis of the transitions. However, the standard DSC still presents some limitations that may reduce the accuracy and precision of measurements. The modulated differential scanning calorimetry (MDSC) has overcome some of these issues by employing sinusoidally modulated heating rates, which are used to determine the heat capacity. Another variant of the MDSC is the supercooling MDSC (SMDSC). SMDSC allows the detection of more complex thermal events such as solid–solid (Ts-s) transitions, liquid–liquid (Tl-l) transitions, and vitrification and devitrification temperatures (Tv and Tdv, respectively), which are typically found at the supercooling temperatures (Tco). The main advantage of MDSC relies on the accurate detection of complex transitions and the possibility of distinguishing reversible events (dependent on the heat capacity) from non-reversible events (dependent on kinetics).

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Section: Food Productsmentioning

confidence: 99%

Application of Differential Scanning Calorimetry (DSC) and Modulated Differential Scanning Calorimetry (MDSC) in Food and Drug Industries

et al. 2019

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“…[5]), determined the variations of the percent of collagen positive area and specific heat capacity values on each fibrosis stage according to Metavir scoring system. Metavir score contemplates five progressive stages: F0, no fibrosis; F1, portal fibrosis; F2, few fibrosis septa; F3, numerous septa; F4, cirrhosis [3].…”

Section: Resultsmentioning

confidence: 99%

“…fibrosis) and fat deposits (i.e. steatosis) in liver may increase on different rate and affect the tissue thermal properties on each fibrosis stage [5,18].…”

Section: Resultsmentioning

confidence: 99%

“…without fibrosis and fat deposits in liver). Additionally, an early thermal analysis showed a linear progressive increase of the specific heat capacity of the hepatic tissue from 30 to 65°C, according to the Metavir score [5]. Starting from the specific heat value in normal conditions and the change rate between the fibrosis stages at 30°C, we calculated the heat capacity values for every Metavir score (see Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…The molecular composition of liver fibrosis consists of the increased extracellular matrix constituents, collagen type I and III, fibronectin, elastin and sulfated proteoglycans [4]. A previous study showed that the accumulation of the extracellular matrix modifies the thermal properties of hepatic tissue on each fibrosis stage, due to the changes on the liver composition and structure [5]. Hence, the development of an optical-integrated sensor for liver fibrosis thermal evaluation is imperative for providing a quantitative evaluation scale, as well as an objective diagnostic tool.…”

Section: Introductionmentioning

confidence: 99%

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Photothermal deflection of laser beam as means to characterize thermal properties of biological tissue: numerical study

et al. 2015

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Non-subjective and early diagnostic technique for liver fibrosis may decrease morbidity in patients and reduce medical costs. Liver fibrosis results in changes in density and thermal properties of tissue. In this work, we evaluate numerically the feasibility of using the optical beam deflection method (OBDM) by means of a thermo-optic material in contact with liver tissue to quantitate changes in thermal conduction. We use the finite-difference method to model the heat transfer in liver and acrylic slab. The response required for thermal characterization for different fibrosis stages is assessed by calculating the deflection angle using ray trace analysis. Numerical study shows the potential of the OBDM for developing an optical-integrated sensor as non-subjective diagnostic technique for liver fibrosis.

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Inhibition of Proprotein Convertase Subtilisin/Kexin Type 9 Ameliorates Liver Fibrosis via Mitigation of Intestinal Endotoxemia

Zou

et al. 2019

Inflammation

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Liver exhibits thermal variations according to the stage of fibrosis progression: A novel use of modulated‐differential scanning calorimetry for research in hepatology

Abstract: Because the liver shows a differential thermal signature according to the degree of fibrosis, mDSC could be a novel tool in the study of liver fibrosis progression.

Cited by 8 publications

References 43 publications

Application of Differential Scanning Calorimetry (DSC) and Modulated Differential Scanning Calorimetry (MDSC) in Food and Drug Industries

Application of Differential Scanning Calorimetry (DSC) and Modulated Differential Scanning Calorimetry (MDSC) in Food and Drug Industries

Photothermal deflection of laser beam as means to characterize thermal properties of biological tissue: numerical study

Inhibition of Proprotein Convertase Subtilisin/Kexin Type 9 Ameliorates Liver Fibrosis via Mitigation of Intestinal Endotoxemia

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