Liver morphology in regulated mechanical jaundice (experimental study)

Abstract

Aim. To examine the morphology of the liver in controlled obstructive jaundice in experimental animalsMaterials and Methods. This research was conducted through an experimental study involving 24 male Shingilla rabbits and 18 white rats of both sexes, all of which were maintained under identical conditions of care and diet. To establish a model of varying degrees of obstructive jaundice, the animals were divided into three groups. Group I comprised animals (n=8) which, following cholecystectomy and drainage of the choledochus, experienced a complete block of hepaticocholedochus within 48 hours. Group II (n=8) included animals wherein the block of hepaticocholedochus persisted for 48-60 hours, and Group III (n=8) involved animals experiencing more than 60 hours of hepaticocholedochus blockage.Results. Histological examination of liver biopsy specimens from the experimental animals revealed distinctive morphological changes in the parenchyma based on the duration of obstructive jaundice modeling, as compared to the liver of a healthy animal. In the pathohistological study of the Group I animals, liver micro-preparations displayed uneven color throughout the entire area of the sections, central vein expansion with altered shape, and traces of a small number of erythrocytes. Analysis of biopsy specimens from Groups II and III revealed irreversible dystrophic and necrotic processes in hepatocytes.Conclusion. Thus, experimental studies, premised on the modeling of controlled obstructive jaundice, revealed that complete blockage of the hepaticocholedochus, over varying time intervals, corresponded with hepatocyte damage, underpinned by escalating cholestatic processes.