Lmod3 promotes myoblast differentiation and proliferation via the AKT and ERK pathways

Lin, Fei-Hu; Wang, An Min; Dai, Wuhou; Chen, Song; Ding, Yahui

doi:10.1016/j.yexcr.2020.112297

Cited by 13 publications

(13 citation statements)

References 44 publications

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“…Several signal pathways are known to regulate myogenic differentiation, such as MEK, ERK, and p70S6K [ 24 , 25 , 26 ]. To examine whether these signals are altered or not by depletion of DPY19L3 gene, we assessed the phosphorylation levels of MEK/ERKs and p70S6K in both parent and C2C12-DPY19L3-KO cells.…”

Section: Resultsmentioning

confidence: 99%

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

et al. 2021

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DPY19L3 has been identified as a C-mannosyltransferase for thrombospondin type-1 repeat domain-containing proteins. In this study, we focused on the role of DPY19L3 in the myogenic differentiation of C2C12 mouse myoblast cells. We carried out DPY19L3 gene depletion using the CRISPR/Cas9 system. The result showed that these DPY19L3-knockout cells could not be induced for differentiation. Moreover, the phosphorylation levels of MEK/ERK and p70S6K were suppressed in the DPY19L3-knockout cells compared with that of parent cells, suggesting that the protein(s) that is(are) DPY19L3-mediated C-mannosylated and regulate(s) MEK/ERK or p70S6K signaling is(are) required for the differentiation.

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Section: Resultsmentioning

confidence: 99%

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

et al. 2021

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“…AKT and ERK are crucial signalling pathways for the maintenance of cellular functions, such as proliferation, angiogenesis, and migration under different conditions [25][26][27][28]. Walker et al reported that the stimulation of endothelial insulin receptors promoted VEGF-A signalling and angiogenesis through ERK1/2 [29].…”

Section: Discussionmentioning

confidence: 99%

G-CSF promotes the viability and angiogenesis of injured liver via direct effects on the liver cells

Liu

Zhang²,

Tong

et al. 2022

Mol Biol Rep

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Background Presently, liver transplantation is the only treatment strategy for liver failure (LF). Although granulocyte-colony stimulating factor (G-CSF) exhibits protective functions in LF, it is not clear whether it directly affects the liver cells. Methods and Results We established an injured liver cell model and observed that G-CSF treatment promoted cell viability and enhanced Ki67 and VEGF-A expression. Thereafter, human umbilical vein endothelial cells (HUVECs) were cultured in a conditioned medium collected from the G-CSF-treated injured liver cells. HUVECs’ proliferation and tubule formation were promoted. Furthermore, in an injured liver mouse model, confirmed via haematoxylin–eosin staining, we evaluated serum alanine aminotransferase activity, Ki67 expression, and microvessel density (MVD). G-CSF treatment significantly relieved liver injury, upregulated Ki67 expression, and enhanced MVD in the injured mouse liver tissue. Additionally, AKT and ERK signal targets were explored, and it was demonstrated that the effects of G-CSF on injured liver cells were mediated through the AKT and ERK signalling pathways. Conclusions G-CSF promotes injured liver viability and angiogenesis by directly affecting injured liver cells via the AKT and ERK signalling pathways. These findings improve our understanding of the role of G-CSF in recovery from LF.

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“…LMOD3, mainly expressed in skeletal muscle, belongs to the Leiomodin protein family [ 46 , 47 ]. Deletion of LMOD3 leads to inhibition of myogenic differentiation as MyoD, MyoG, and MyHC are significantly suppressed in C2C12 cells [ 48 ]. LMOD3 was associated with skeletal muscle development due to its more abundant level in Yorkshire pigs than Beijing Black pigs.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Expression Profiling of mRNAs, lncRNAs and circRNAs in Skeletal Muscle of Two Different Pig Breeds

Hou

Wang

Zhao

et al. 2021

Animals

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RNA-Seq technology is widely used to analyze global changes in the transcriptome and investigate the influence on relevant phenotypic traits. Beijing Black pigs show differences in growth rate and meat quality compared to western pig breeds. However, the molecular mechanisms responsible for such phenotypic differences remain unknown. In this study, longissimus dorsi muscles from Beijing Black and Yorkshire pigs were used to construct RNA libraries and perform RNA-seq. Significantly different expressions were observed in 1051 mRNAs, 322 lncRNAs, and 82 circRNAs. GO and KEGG pathway annotation showed that differentially expressed mRNAs participated in skeletal muscle development and fatty acid metabolism, which determined the muscle-related traits. To explore the regulatory role of lncRNAs, the cis and trans-target genes were predicted and these lncRNAswere involved in the biological processes related to skeletal muscle development and fatty acid metabolismvia their target genes. CircRNAs play a ceRNA role by binding to miRNAs. Therefore, the potential miRNAs of differentially expressed circRNAs were predicted and interaction networks among circRNAs, miRNAs, and key regulatory mRNAs were constructed to illustrate the function of circRNAs underlying skeletal muscle development and fatty acid metabolism. This study provides new clues for elucidating muscle phenotypic variation in pigs.

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Lmod3 promotes myoblast differentiation and proliferation via the AKT and ERK pathways

Cited by 13 publications

References 44 publications

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

Involvement of DPY19L3 in Myogenic Differentiation of C2C12 Myoblasts

G-CSF promotes the viability and angiogenesis of injured liver via direct effects on the liver cells

Genome-Wide Expression Profiling of mRNAs, lncRNAs and circRNAs in Skeletal Muscle of Two Different Pig Breeds

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