Lnc-MAP6-1:3 knockdown inhibits osteosarcoma progression by modulating Bax/Bcl-2 and Wnt/β-catenin pathways

Abstract

Osteosarcoma (OS) is the most common type of malignant bone tumor that affects children and adolescents. Still, the cellular and molecular mechanisms driving the development of this disease remain poorly understood. In this study, numerous dysregulated lncRNAs were identified by RNA-seq. As a result, we were able to find a novel lncRNA Lnc-MAP6-1:3 which is highly expressed in osteosarcoma. Using a set of approaches including gene knockdown, RT-PCR, oncogenic function assay and western blotting, we observed that knockdown of Lnc-MAP6-1:3 expression suppressed cell proliferation and colony formation, and promoted apoptosis in vitro. For the first time, we have identified that Lnc-MAP6-1:3 potentially influence the malignant behavior of osteosarcoma via Bax/Bcl-2 and Wnt/β-catenin signaling pathways. Henceforth, Lnc-MAP6-1:3 may provide a new molecular route of research and therapeutic applications for the diagnosis and treatment of osteosarcoma.

Anti-Myocardial Ischemia Reperfusion Injury Mechanism of Dried Ginger-Aconite Decoction Based on Network Pharmacology

“…Network pharmacological analysis implied that the molecular mechanism of the anti-MI/RI effect of DAD might be associated with apoptosis. The mammalian BCL-2 family member Bcl-2 was an anti-apoptotic protein, while Bax protein induced apoptosis by enhancing cytochrome c (Cyt-C) release from the mitochondria ( Aamazadeh et al, 2020 ; Lin et al, 2020 ). Therefore, the two targets of Bax and Bcl2 (Bax and Bcl2 belonged to the targets of DAD in anti-MI/RI) were validated in vitro .…”

Therapeutic Effects of Zoledronic Acid-Loaded Hyaluronic Acid/Polyethylene Glycol/Nano-Hydroxyapatite Nanoparticles on Osteosarcoma

“…The antiapoptotic protein Bcl-2, mainly located in the outer membrane of the mitochondria, was upregulated in osteosarcoma to inhibit the apoptosis induced by various stimuli, injury, and cell survival signals ( Valentin et al, 2018 ). Bax is an important pro-apoptotic gene ( Lin et al, 2020 ). When overexpressed, Bax can bind to homologous dimers and promote apoptosis.…”

Biomarker potential of lncRNA GNAS-AS1 in osteosarcoma prognosis and effect on cellular function

“…Elucidation of the molecular basis on which lncRNAs affect OS development and progression is essential to establish better treatment options [1,28]. Recently, lncRNAs have been identified that participate in OS progression, including cancerous cell growth, metastasis, and apoptosis [29][30][31]. LncRNA GNAS-AS1 was an important gene related to the formation and progression of nasopharyngeal carcinoma and breast cancer [17,18].…”