LncNONO-AS regulates AR expression by mediating NONO

Zhao, Zhongquan; Qiao, Lei; Dai, Zinuo; He, Qijie; Lan, Xi; Huang, Siyi; He, Lin

doi:10.1016/j.theriogenology.2019.10.025

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…ar is an important receptor in the supporting cells of the testis. It plays an important role in maintaining the number of spermatogonia and the integrity of the blood testis barrier, in the completion of meiosis, in the adhesion of sperm cells and in the fertilization of sperm and other spermatogenesis processes [ 43 , 44 ]. In this study, ar was enriched in only IL vs. TE, indicating that it plays an important role in the development of the testis during the sex reversal process of M. albus .…”

Section: Discussionmentioning

confidence: 99%

Identification, characterization and functional analysis of gonadal long noncoding RNAs in a protogynous hermaphroditic teleost fish, the ricefield eel (Monopterus albus)

Yang

et al. 2022

BMC Genomics

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Background An increasing number of long noncoding RNAs (lncRNAs) have been found to play important roles in sex differentiation and gonad development by regulating gene expression at the epigenetic, transcriptional and posttranscriptional levels. The ricefield eel, Monopterus albus, is a protogynous hermaphroditic fish that undergoes a sequential sex change from female to male. However, the roles of lncRNA in the sex change is unclear. Results Herein, we performed RNA sequencing to analyse lncRNA expression patterns in five different stages of M. albus development to investigate the roles of lncRNAs in the sex change process. A total of 12,746 lncRNAs (1503 known lncRNAs and 11,243 new lncRNAs) and 2901 differentially expressed lncRNAs (DE-lncRNAs) were identified in the gonads. The target genes of the DE-lncRNAs included foxo1, foxm1, smad3, foxr1, camk4, ar and tgfb3, which were mainly enriched in signalling pathways related to gonadal development, such as the insulin signalling pathway, MAPK signalling pathway, and calcium signalling pathway. We selected 5 highly expressed DE-lncRNAs (LOC109952131, LOC109953466, LOC109954337, LOC109954360 and LOC109958454) for full length amplification and expression pattern verification. They were all expressed at higher levels in ovaries and intersex gonads than in testes, and exhibited specific time-dependent expression in ovarian tissue incubated with follicle-stimulating hormone (FSH) and human chorionic gonadotropin (hCG). The results of quantitative real-time PCR (qRT-PCR) analysis and a dual-luciferase assay showed that znf207, as the gene targeted by LOC109958454, was expressed in multiple tissues and gonadal developmental stages of M. albus, and its expression was also inhibited by the hormones FSH and hCG. Conclusions These results provide new insights into the role of lncRNAs in gonad development, especially regarding natural sex changes in fish, which will be useful for enhancing our understanding of sequential hermaphroditism and sex changes in the ricefield eel (M. albus) and other teleosts.

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Section: Discussionmentioning

confidence: 99%

Identification, characterization and functional analysis of gonadal long noncoding RNAs in a protogynous hermaphroditic teleost fish, the ricefield eel (Monopterus albus)

Yang

et al. 2022

BMC Genomics

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“…For example, our previous study showed that high expression of ANO1‐AS2 downregulated the ANO1 gene by interacting with the ANO1 gene promoter and influenced sperm motility and morphology (Saberiyan, Mirfakhraie, Gholami, et al, 2020). Moreover, it was shown that lncNONO‐AS regulated spermatogenesis in goats by regulating androgen receptor (AR) expression (Zhao et al, 2020). Furthermore, it was revealed that SLC7A11‐AS1 is associated with varicocele‐related male infertility (Sanei‐Ataabadi et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Reduced expression ofCFAP44andCFAP44‐AS1may affect sperm motility and morphology

et al. 2022

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Motility and morphology are two important characteristics of a fertile spermatozoon. CFAP44 gene encodes flagellar protein 44 involved in the formation and function of the flagella and cilia. Long non‐coding RNAs are regulatory elements involved in several processes, including reproduction. We aimed to study the alterations in the expressions of CFAP44 and CFAP44‐AS1 genes in infertile men with asthenozoospermia and terato‐asthenozoospermia. In this case–control study, a total of 105 subjects, including 35 TAZ patients, 34 AZ patients and 35 normozoospermic men, were enrolled. After RNA extraction from spermatozoa samples, quantitative real‐time PCR was performed to compare the expression of CFAP44 and CFAP44‐AS1 between the studied groups. A meaningful reduction in CFAP44 expression and a significant reduction in the expression of CFAP44‐AS1 were observed. Moreover, a positive correlation between both genes' expressions and normal sperm morphology was detected in NZ, AZ and TAZ groups. Also, there was a positive relation between CFAP44 gene expression and sperm motility in AZ and TAZ groups. The expression of CFAP44‐AS1 was positively correlated with sperm motility and morphology. Present results confirm the role of CFAP44 and CFAP44‐AS1 in the motility and morphology of spermatozoon, and deregulation of these genes may contribute to male infertility.

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“…This lncRNA is mainly expressed in the nucleus and plays an important role at the epigenetic level. It also regulates spermatogenesis and testicular development by regulating the expression of the androgen receptor (AR) [119]. The overexpression of lncNONO-AS can increase the expression of AR by mediating NONO.…”

Section: Lncrnas In Livestockmentioning

confidence: 99%

“…All of these lncRNAs were expressed in sperm from infertile men and were intercorrelated, thus suggesting that they may be associated with sperm viability. To better understand the reasons for spermatogenic failure in humans, Jan et al [119] created a transcriptomic dataset of distinct and well-defined germ cell subtypes (deposited in NCBI's Sequence Read Archive under accession number SRP069329). Using next-generation RNA sequencing, these authors also found that 137 lncRNAs and 110 RNA-binding proteins were significantly expressed in specific cells in human testis samples [135].…”

Section: Lncrnas In Humansmentioning

confidence: 99%

Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets

Zhao

Heng

Sahlu

et al. 2021

IJMS

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Long noncoding RNAs (lncRNAs) are composed of nucleotides located in the nucleus and cytoplasm; these are transcribed by RNA polymerase II and are greater than 200 nt in length. LncRNAs fulfill important functions in a variety of biological processes, including genome imprinting, cell differentiation, apoptosis, stem cell pluripotency, X chromosome inactivation and nuclear transport. As high throughput sequencing technology develops, a substantial number of lncRNAs have been found to be related to a variety of biological processes, such as development of the testes, maintaining the self-renewal and differentiation of spermatogonial stem cells, and regulating spermatocyte meiosis. These indicate that lncRNAs can be used as biomarkers and potential therapeutic targets for male infertility. However, only a few comprehensive reviews have described the role of lncRNAs in male reproduction. In this paper, we summarize recent findings relating to the role of lncRNAs in spermatogenesis, their potential as biomarkers for male infertility and the relationship between reproductive arrest and transgenerational effects. Finally, we suggest specific targets for the treatment of male infertility from the perspective of lncRNAs.

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LncNONO-AS regulates AR expression by mediating NONO

Cited by 10 publications

References 18 publications

Identification, characterization and functional analysis of gonadal long noncoding RNAs in a protogynous hermaphroditic teleost fish, the ricefield eel (Monopterus albus)

Identification, characterization and functional analysis of gonadal long noncoding RNAs in a protogynous hermaphroditic teleost fish, the ricefield eel (Monopterus albus)

Reduced expression ofCFAP44andCFAP44‐AS1may affect sperm motility and morphology

Long Noncoding RNAs: Recent Insights into Their Role in Male Infertility and Their Potential as Biomarkers and Therapeutic Targets

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