LncRNA AFAP1-AS Functions as a Competing Endogenous RNA to Regulate RAP1B Expression by sponging miR-181a in the HSCR

Chen, Guanglin; Peng, Lei; Zhu, Zezhang; Du, Chong; Zheng, Shen; Zang, Rujin; Su, Yang; Xia, Yankai; Tang, Weibing

doi:10.7150/ijms.18392

Cited by 30 publications

(26 citation statements)

References 28 publications

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“…Using TNM I stage lung adenocarcinoma samples in the TCGA database, Tian et al ( 40 ) demonstrated that FENDRR and LINC00312 had diagnostic value in patients with LUAD. A recent study has demonstrated that lncRNA can act as a ceRNA that adsorbs onto miRNAs to regulate mRNA expression ( 41 ). The ceRNA hypothesis is a novel regulatory mechanism that serves a role through miRNA competition ( 10 , 42 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of ceRNA network based on a RNA‑seq shows prognostic lncRNA biomarkers in human lung adenocarcinoma

Ran

et al. 2018

Oncol Lett

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Previous studies have emphasized the significant functions of long non-coding RNAs (lncRNAs) as competing endogenous RNAs (ceRNAs) in tumor biology. However, the functions of certain cancer lncRNAs in the lncRNA-related ceRNA network in lung adenocarcinoma (LUAD) are unknown. A systematic and integrative survey of RNA-seq data from The Cancer Genome Atlas (TCGA) was performed to identify candidate lncRNAs for the prognosis of LUAD. In total, 20,502 genes that contain 181 lncRNAs were evaluated in a cohort of 570 LUAD cases. Initially, 6,280 differentially expressed genes (fold-change >2, P<0.05) were obtained using R package, which includes 75 lncRNAs. Next, by univariate regression and multivariate Cox proportional hazards analysis, 32 genes were associated with survival in LUAD. Using these 29 mRNAs and 3 lncRNAs, a prognosis index (PI) was calculated to accurately estimate the survival in LUAD: PI=∑exprisk gene × HRrisk gene. Furthermore, the 32-gene signature was an independent prognostic indicator for LUAD (HR >1; P<0.05, by multivariate analysis). Weighted gene co-expression network analysis (WGCNA) of three risk lncRNAs-FAM138B, NHEG1 and TLX1NB-was performed, based on the P-values of the associated genes, and the top 27 miRNAs that bound to these lncRNAs were predicted by Miranda as target miRNAs. Next, these target miRNAs were transferred to the TarBase, miRTarBase, miRecards and starBase v2.0 databases to obtain their target genes. According to the previous miRNA-mRNA and miRNA-lncRNA data, three lncRNA-miRNA-mRNA ceRNA networks were established, based on the 29 prognostic mRNAs, forming a regulatory network in LUAD. The present study provided insight into the lncRNA-related ceRNA network in LUAD and has identified potential diagnostic and prognostic biomarkers.

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Section: Discussionmentioning

confidence: 99%

Identification of ceRNA network based on a RNA‑seq shows prognostic lncRNA biomarkers in human lung adenocarcinoma

Ran

et al. 2018

Oncol Lett

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“…Much evidence suggests a relationship between lncRNAs and HSCR, all based on the comparative expression assays of specific lncRNAs in bowel tissue from HSCR patients and controls [73][74][75][76][77][78]. In our study we have performed a differential qRT-PCR assay in the EPCs from HSCR patients and controls, which has allowed us to identify new lncRNAs as potential regulatory elements implicated in ENS development and HSCR onset.…”

Section: Discussionmentioning

confidence: 99%

Identification of New Potential LncRNA Biomarkers in Hirschsprung Disease

Torroglosa

Villalba‐Benito

Fernández

et al. 2020

IJMS

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Hirschsprung disease (HSCR) is a neurocristopathy defined by intestinal aganglionosis due to alterations during the development of the Enteric Nervous System (ENS). A wide spectrum of molecules involved in different signaling pathways and mechanisms have been described in HSCR onset. Among them, epigenetic mechanisms are gaining increasing relevance. In an effort to better understand the epigenetic basis of HSCR, we have performed an analysis for the identification of long non-coding RNAs (lncRNAs) by qRT-PCR in enteric precursor cells (EPCs) from controls and HSCR patients. We aimed to test the presence of a set lncRNAs among 84 lncRNAs in human EPCs, which were previously related with crucial cellular processes for ENS development, as well as to identify the possible differences between HSCR patients and controls. As a result, we have determined a set of lncRNAs with positive expression in human EPCs that were screened for mutations using the exome data from our cohort of HSCR patients to identify possible variants related to this pathology. Interestingly, we identified three lncRNAs with different levels of their transcripts (SOCS2-AS, MEG3 and NEAT1) between HSCR patients and controls. We propose such lncRNAs as possible regulatory elements implicated in the onset of HSCR as well as potential biomarkers of this pathology.

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“…As a result of the limitation of the enteric nervous system (ENS) cell model of HSCR, according to previous reporting papers, two most felicitous cell lines, human SKN-SH and SH-SY5Y cells, are neuroblastoma cell lines which derived from NCCs and used to investigate the mechanism of HSCR in many articles. 23–25 These two cell lines were obtained from Dr. Qiangsong Tong, at Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, China. 26 These two cell lines were used via in vitro experiments and cultivated in a humidified atmosphere of 5% CO 2 , at 37 °C, with DMEM containing high glucose levels (Boster, Wuhan, China), 10% fetal bovine serum (FBS) (Sciencell, Beijing, China), streptomycin (100 μg/mL) and penicillin (100 U/mL) (Sigma-Aldrich, St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with PLAGL2

Yuan

Chen

et al. 2019

Pediatr Res

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BackgroundThe miR-214 has been reported to be associated with various diseases, but its involvement in the pathophysiology of Hirschsprung disease (HSCR) is almost completely unexplored.MethodsIn our study, we conducted a series of experiments to unravel the biological role of miR-214 in the pathophysiology of HSCR. qRT-PCR and western blotting were utilized to investigate the relative expression levels of miR-214, mRNAs, and proteins of related genes in colon tissues from 20 controls without HSCR and 24 patients with HSCR. The potential biological role of miR-214 in two cell lines (SKN-SH and SH-SY5Y) was assessed using the CCK8 assay, EdU staining, transwell assay, and flow cytometry. The dual-luciferase reporter assay was used to confirm PLAGL2 as a common target gene of miR-214.ResultsAll results suggested that miR-214 is upregulated in HSCR tissue samples compared with controls. Additionally, we found that miR-214 could inhibit cell proliferation and migration by directly downregulating the expression of PLAGL2, and the extent of the miR-214-mediated inhibitory effects could be rescued by a PLAGL2 overexpression plasmid.ConclusionOur results revealed that miR-214 is indeed involved in the pathophysiology of HSCR and suppresses cell proliferation and migration by directly downregulating PLAGL2 in cell models.

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LncRNA AFAP1-AS Functions as a Competing Endogenous RNA to Regulate RAP1B Expression by sponging miR-181a in the HSCR

Cited by 30 publications

References 28 publications

Identification of ceRNA network based on a RNA‑seq shows prognostic lncRNA biomarkers in human lung adenocarcinoma

Identification of ceRNA network based on a RNA‑seq shows prognostic lncRNA biomarkers in human lung adenocarcinoma

Identification of New Potential LncRNA Biomarkers in Hirschsprung Disease

Increased miR-214 expression suppresses cell migration and proliferation in Hirschsprung disease by interacting with PLAGL2

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