LncRNA DANCR improves the dysfunction of the intestinal barrier and alleviates epithelial injury by targeting the miR‐1306‐5p/PLK1 axis in sepsis

Abstract

Intestinal barrier dysfunction often occurs in various acute or chronic pathological conditions and has been identified as an important clinical problem. Herein, we explored the biological role and molecular mechanism of Polo‐like kinase 1 (PLK1) and differentiation antagonizing non‐protein coding RNA (DANCR) in intestinal barrier dysfunction caused by sepsis. RT‐qPCR analysis was used to examine PLK1, miR‐1306‐5p, and DANCR expression in NCM460 cells after LPS treatment. TUNEL assay and Western blot analysis were performed to explore PLK1 function in cell apoptosis and intestinal barrier in vitro. Hematoxylin and eosin staining, Western blot analysis, and TUNEL assay were used to investigate DANCR function in the intestinal barrier and cell apoptosis in vivo. The interaction between miR‐1306‐5p and PLK1 (or DANCR) was validated by luciferase reporter assay. As a result, PLK1 overexpression decreased cell apoptosis and promoted intestinal barrier function. Moreover, DANCR was validated as a sponge of miR‐1306‐5p to target PLK1. In addition, we found that DANCR overexpression decreased intestinal mucosal permeability and colon mucosa epithelial cell apoptosis in vivo. Conclusively, DANCR improved intestinal barrier dysfunction and alleviated epithelial injury by targeting the miR‐1306‐5p/PLK1 axis in sepsis.

MicroRNA-1306-5p Regulates the METTL14-Guided m6A Methylation to Repress Acute Myeloid Leukemia

“…Several miRNAs are also found to play crucial roles in AML by regulating various kinds of biological processes, such as miRNA-485-5p, miR-126, and miR-204 [12][13][14]. In addition, as reported previously, miR-1306-5p is involved in the progression of many diseases, such as amelogenesis imperfecta, cerebral ischemia/reperfusion injury, and sepsis [15][16][17]. However, the role of miR-1306-5p in AML is obscure.…”

lnc001776 Affects CPB2 Toxin-Induced Excessive Injury of Porcine Intestinal Epithelial Cells via Activating JNK/NF-kB Pathway through ssc-let-7i-5p/IL-6 Axis

“…An increasing amount of evidence indicated that lncRNAs can influence cellular functions by regulating mRNAs through adsorption of corresponding miRNAs [ 51 ]. lncRNA DANCR targeting miR-1306-5p/polo-like Kinase 1 ( PLK1 ) axis reduced intestinal mucosal permeability and apoptosis of colonic mucosal epithelial cells and improved intestinal barrier dysfunction [ 52 ]. lncRNA TUG1 overexpression, targeting the miR-186-5p/X-linked inhibitor of apoptosis protein ( XIAP ) axis, attenuated NLRP3 inflammatory vesicle-induced cardiomyocyte scorching [ 53 ].…”