LncRNA HOXA10-AS Activated by E2F1 Facilitates Proliferation and Migration of Nasopharyngeal Carcinoma Cells Through Sponging miR-582-3p to Upregulate RAB31

Abstract

Nasopharyngeal carcinoma (NPC) is a kind of head and neck cancer with a characteristic regional distribution. Increasing evidence has illustrated that long noncoding RNAs (lncRNAs) exert the regulatory function in tumor development. Nevertheless, the specific functions of lncRNA HOXA10 antisense RNA (HOXA10-AS) in NPC remain to be clarified. In this research, quantitative reverse transcription polymerase chain reaction detected HOXA10-AS expression in NPC cells. Cell counting kit-8, colony formation, and transwell assays were utilized to measure the proliferation and migration of NPC cells. Moreover, mechanism assays detected the interaction of different genes. Briefly, HOXA10-AS was highly expressed in NPC cells. HOXA10-AS down-regulation restrained NPC cell proliferation and migration. Further, HOXA10-AS could bind to miR-582-3p by acting as a competing endogenous RNA. Besides, Ras-related protein Rab-31 (RAB31) was proven as the target gene of miR-582-3p. Additionally, E2F transcription factor 1 (E2F1) acted as a transcription factor to activate HOXA10-AS expression. In the final rescue assays, we observed that the effect of HOXA10-AS depletion on NPC cell growth could be fully reversed by RAB31 overexpression or miR-582-3p inhibition. In short, our research proved that HOXA10-AS activated by E2F1 facilitated proliferation and migration of NPC cells through sponging miR-582-3p to upregulate RAB31.

CDC25A inhibition suppresses cell proliferation and induces G1/S‑phase cell cycle arrest in nasopharyngeal carcinoma

“…an number of studies have highlighted the crucial role of e2F1 in various types of malignancies (12), including ovarian cancer (13), prostate cancer (14) and glioblastoma (15). it is noteworthy that the importance of e2F1 in NPC inflammation and tumorigenesis has also been identified in previously published studies (16,17). nevertheless, whether cdc25a functions in nPc via transcriptional regulation of e2F1 remains unclear.…”

Crosstalk between Long Non-Coding RNA and Spliceosomal microRNA as a Novel Biomarker for Cancer

“…Decrease in the level of lncRNA HOXA10-AS reduced the sponging of mir-340-3p, resulting in an increase in mir-340-3p and a subsequent decrease in HTR1D to ultimately suppress the malignancy of cancer [ 51 ]. Also, HOXA10-AS is highly expressed in nasopharyngeal carcinoma (NPC) cells [ 55 ]. It was demonstrated that HOXA10-AS, activated by E2F1, leads to an increase in the proliferation and migration of NPC cells, through the sponging of mir-582-3p.…”

“…It was demonstrated that HOXA10-AS, activated by E2F1, leads to an increase in the proliferation and migration of NPC cells, through the sponging of mir-582-3p. This sponging of mir-582-3p leads to upregulation of its target gene Ras-related protein Rab-31 (RAB31) and facilitates cell proliferation and migration of NPC cells [ 55 ].…”

Science and Research March Onward

“…Finally, Wang et al delve into the regulation of tumor development in nasopharyngeal carcinoma (NPC). 14 More precisely, they examine the role of long noncoding RNA HOXA10 antisense RNA in the proliferation and migration of NPC cells using several techniques including quantitative reverse transcription PCR and transwell assays. This article contributes to our understanding of the pathogenesis of NPC, and illustrates the diversity of research brought to the forefront by the American Journal of Rhinology and Allergy.…”