LncRNA OIP5-AS1 promotes the malignancy of pancreatic ductal adenocarcinoma via regulating miR-429/FOXD1/ERK pathway

Wu, Liping; Liu, Yongcun; Guo, Cheng; Shao, Yuan

doi:10.1186/s12935-020-01366-w

Cited by 39 publications

(26 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 10 LncRNA OIP5-AS1 overexpression contributes to proliferation, migration, and invasion in pancreatic ductal adenocarcinoma. 11 In addition, LINC01614 upregulation sponges miR-383 to promote glioma growth and invasion by affecting ADAM12 expression. 12 Therefore, illustrating the biological roles and molecular mechanisms of lncRNAs is of important significance.…”

Section: Introductionmentioning

confidence: 99%

LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

Zheng¹,

Xie²,

Xu³

et al. 2021

OTT

View full text Add to dashboard Cite

Background: Long noncoding RNAs (lncRNA) exert essential functions during tumorigenesis. However, how lncRNAs participate in glioma development remains poorly researched. This study aimed to determine how DDX11-AS1 affects glioma progression. Methods: Gene expression was analyzed by qRT-PCR. Survival rate curve was plotted in 56 glioma patients. Loss-of-function assays were performed to analyze proliferation, migration, and invasion through CCK8, colony formation, and transwell assays. Luciferase assay and RNA pulldown assays were conducted to illustrate the underlying molecular mechanism. Results: DDX11-AS1 expression was upregulated in glioma tissues and cells. DDX11-AS1 overexpression was linked with poor prognostic value. DDX11-AS1 knockdown suppressed proliferation, migration, and invasion while inducing apoptosis. DDX11-AS1 interacted with miR-499b-5p to eliminate it, leading to upregulation of RWDD4 expression. RWDD4 was upregulated in glioma while miR-499b-5p was downregulated. Conclusion: DDX11-AS1 upregulation promotes glioma progression through acting as a competing endogenous RNA for miR-499b-5p to upregulate RWDD4.

show abstract

Section: Introductionmentioning

confidence: 99%

LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

Zheng¹,

Xie²,

Xu³

et al. 2021

OTT

View full text Add to dashboard Cite

show abstract

“…OIP5-AS1 may sponge miR-34a, and then upregulate the expression of its downstream target snail, thereby promoting invasion and migration of OC cells. OIP5-AS1 has been characterized as an oncogenic lncRNA in many types of cancer [16][17][18]. In lung cancer, OIP5-AS1 targets miR-448 and Bcl-2 to regulate the proliferation and apoptosis of cancer cells [14].…”

Section: Discussionmentioning

confidence: 99%

“…LncRNAs are abnormally expressed in a variety of human malignant tumors, and these abnormally expressed LncRNAs can be used as biomarkers for early clinical diagnosis. Recently, it has found that OIP5-AS1, as a novel lncRNA, is abnormally expressed in multiple tumors and can be used as a clinical biomarker for liver hepatocellular carcinoma and adenocarcinoma [ 16 , 17 ]. However, its expression pattern and biological role in OC have not been well studied.…”

Section: Discussionmentioning

confidence: 99%

LncRNA OIP5-AS1 upregulates snail expression by sponging miR-34a to promote ovarian carcinoma cell invasion and migration

Jiang

et al. 2020

Biol Res

View full text Add to dashboard Cite

Background Although OIP5-AS1 has been characterized as an oncogenic lncRNA in many types of cancer, its role and underlying mechanism in ovarian carcinoma (OC) remains unknown. This study aimed to investigate the role of OIP5-AS1 in OC. Methods OC tissues and non-tumor tissues (ovary tissues within 3 cm around tumors) were collected from 58 OC patients (age range 36 to 67 years old, mean age 51.4 ± 5.9 years old). The expression of OIP5-AS1 and snail in paired tissues were determined by RT-qPCR. The interaction between OIP5-AS1 and miR-34a was predicted by IntaRNA2.0 and confirmed by dual luciferase reporter assay. The effects of overexpression of OIP5-AS1 and miR-34a on the expression of snail were analyzed by RT-qPCR and Western blotting. Cell invasion and migration were analyzed by Transwell assay. Results We observed that the expression of OIP5-AS1 and snail was upregulated and positively correlated with each other in OC. RNA–RNA interaction analysis showed that OIP5-AS1 might sponge miR-34a. In OC cells, overexpression of OIP5-AS1 resulted in the upregulated expression of snail, while overexpression of miR-34a downregulated the expression of snail. In addition, overexpression of miR-34a reduced the effects of overexpression of OIP5-AS1 on the expression of snail. In cell invasion and migration assay, overexpression of OIP5-AS1 and snail resulted in increased OC cell invasion and migration, while overexpression of miR-34a decreased OC cell invasion and migration. Moreover, overexpression of miR-34a attenuated the effects of OIP5-AS1 overexpression on OC cell invasion and migration. Conclusions Therefore, OIP5-AS1 may upregulate snail expression in OC by sponging miR-34a to promote OC cell invasion and migration.

show abstract

“…The knockdown of OIP5-AS1 inhibited the proliferation, invasion and migration of ovarian cancer cells, and promoted the apoptosis of ovarian cancer cells to a certain extent. OIP5-AS1 has been identi ed as an oncogene of human diseases in a variety of cancer cells, such as breast cancer cells [30] , non-small cell lung cancer cells [5] , pancreatic ductal carcinoma cells [29] , and multiple myeloma cells [28] .…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, lncRNAs play their roles through a variety of complex molecular mechanisms [20] , such as affecting the epigenetic activity of tumor cells in the nucleus, gene transcription and post-transcriptional modi cation of mRNA [2] . LncRNA OIP5-AS1 has been reported to inhibit the proliferation of multiple malignant tumors [29,28] . It can regulate a variety of biological activities of cells in some diseases and tumors.…”

Section: Introductionmentioning

confidence: 99%

LncRNA OIP5-AS1 Affects the Malignant Biological Behavior of Ovarian Cancer via the miR-153-3p/KLF5 Axis

Wang

Liu

et al. 2020

Preprint

View full text Add to dashboard Cite

Objectives: The purpose of this study was to investigate the expression and clinical significance of LncRNA OIP5-AS1 in ovarian cancer , as well as its effect on malignant biological behavior of ovarian cancer cells. Methods: The expression of OIP5-AS1, miR-153-3p and KLF5 in ovarian cancer (OC) tissues and cells were detected by RT-qPCR. Western Blotting was used to detect KLF5 expression. The expression patterns of OIP5-AS1, U6 and GAPDH in nuclear and cytoplasm fractions were detected using qRT-PCR. Besides, CCK-8 assay, clone formation assay, transwell, scratch test, and flow cytometry were respectively used to detect the cell activity, proliferation, invasiveness, healing of cells, and apoptosis rate of OC cells. Furthermore, The interaction between OIP5-AS1 and miR-153-3p and between miR-153-3p and KLF5 were verified by luciferase reporter assay, and the correlations among these three genes were analyzed.Results: OIP5-AS1 expression was up-regulated in ovarian cancer cell lines and tissues. Si-OIP5-AS1 inhibited cell proliferation, invasion and migration, and induced the apoptosis to a certain extent. Subcellular fraction assay revealed the location of OIP5-AS1 was mainly situated in the cytoplasm. In addition, miR-153-3p was a target of OIP5-AS1, and KLF5 was directly targeted by miR-153-3p. Si-OIP5-AS1 inhibited KLF5 expression, miR-153-3p inhibitor promoted KLF5 expression, and si-KLF5 inhibited OIP5-AS1 expression. Interestingly, expression of OIP5-AS1 and miR-153-3p, and expression of miR-153-3p and KLF5 were negatively correlated, while expression of OIP5-AS1 and KLF5 was positively correlated. In addition, si-KLF5 inhibited the malignant biological behavior of ovarian cancer cells, while miR-153-3p inhibitor had the opposite effect. Most importantly, the addition of si-OIP5-AS1 to mir-153-3p silenced cells could reverse the promotion effect of miR-153-3p inhibitor on the malignant biological behavior of ovarian cancer cells.Conclusions: OIP5-AS1 can be used as an effective prognostic indicator of ovarian cancer, which has the potential to be a new drug target.

show abstract

LncRNA OIP5-AS1 promotes the malignancy of pancreatic ductal adenocarcinoma via regulating miR-429/FOXD1/ERK pathway

Cited by 39 publications

References 43 publications

LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

LncRNA DDX11-AS1 Exerts Oncogenic Roles in Glioma Through Regulating miR-499b-5p/RWDD4 Axis

LncRNA OIP5-AS1 upregulates snail expression by sponging miR-34a to promote ovarian carcinoma cell invasion and migration

LncRNA OIP5-AS1 Affects the Malignant Biological Behavior of Ovarian Cancer via the miR-153-3p/KLF5 Axis

Contact Info

Product

Resources

About