LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA

Chen, Mingwei; Zhang, Rongyuan; Lu, Le; Du, Jian; Chen, Chunyang; Ding, Keke; Wei, Xuedong; Zhang, Guangbo; Huang, Yuhua; Hou, Jianquan

doi:10.18632/aging.202203

Cited by 37 publications

(30 citation statements)

References 61 publications

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“…Cervical cancer, as one of the common human tumors, is one of the most common gynecological malignancies, 1–5 , 34 which is like other tumors 35–41 and they threatened seriously to human health as well as cervical cancer, 42–48 its morbidity and mortality rate are at the forefront of female malignancies. Surgery, radiotherapy and chemotherapy for early cervical cancer have good effects, but in advanced stage, the therapeutic effect of metastatic and recurrent cervical cancer is very limited and the survival rate is low.…”

Section: Discussionmentioning

confidence: 99%

<p>Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer</p>

Shi¹,

Wang²,

Dai³

et al. 2020

OTT

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Background Previous reports showed that APOC1 was associated with several cancers but the function of APOC1 in cervical cancer was unknown. This study aimed to investigate the clinical effect and function of APOC1 in cervical cancer. Materials and Methods In this study, the relative expression of APOC1 in cervical cancer was detected by RT-qPCR. In order to determine the cell proliferation and migration and invading ability and apoptosis more accurately, we used CCK8 assay, Edu assay, wound healing assay, migration and invasion assay, flow cytometry assay, co-immunoprecipitation, proteomics and Western blot by silencing and overexpressing APOC1 , respectively. The role of APOC1 on tumor progression was explored in vitro and vivo. Results The relative expression of APOC1 in cervical cancer tissues was up-regulated (P<0.05). In cervical cancer cell lines, silencing of APOC1 restrained cell progression and EMT, while over-expression of APOC1 accelerated cell progression and EMT in vivo and vitro (P<0.05). Conclusion APOC1 acts as an oncogene in cervical cancers and knockdown of APOC1 inhibited cervical cancer cells growth in vitro and in vivo. There is a close relationship between the relative expression of APOC1 and clinical outcome in cervical cancer patients.

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Section: Discussionmentioning

confidence: 99%

<p>Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer</p>

Shi¹,

Wang²,

Dai³

et al. 2020

OTT

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show abstract

“…Generally, lncRNAs show biological functions by sponging miRNAs, which normally degrade their target genes by binding to their 3'-UTR (26). miR-30a-5p expression is downregulated in renal cancer, and its overexpression ameliorates the malignant phenotypes of renal carcinoma cells by degrading SOX4 (27). miR-30a has been considered as a potential prognostic biomarker for clear cell renal cell carcinoma, and it inhibits cell migration and invasion of HCT116 cells (28).…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression

Chen

Fang

et al. 2021

Oncol Lett

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Long non-coding RNAs (lncRNAs) serve an important role in the development of esophageal cancer (EC), which is the eighth most common type of cancer worldwide. lncRNA opa-interacting protein 5 antisense transcript 1 (OIP5-AS1) is associated with human malignancy. However, the biological roles of OIP5-AS1 in the development of EC remain unclear. In the present study, transfection was conducted, and reverse transcription-quantitative PCR and western blot analysis were used for the detection of mRNA and protein expression, respectively. Furthermore, dual-luciferase reporter and RNA immunoprecipitation assays were used to study the interaction between miRNA and lncRNA or genes. The results revealed that OIP5-AS1 expression in EC tissues and cultured EC cells was upregulated, microRNA-30a (miR-30a) expression was downregulated. OIP5-AS1-knockdown suppressed the proliferation, migration and invasion of EC9706 and EC109 cells. miR-30a was confirmed to interact with OIP5-AS1, and miR-30a-mimics transfection ameliorated the effects of OIP5-AS1 in EC cells. Vesicular overexpressed in cancer prosurvival protein 1 (VOPP1) was verified as the direct target of miR-30a. VOPP1 expression was positively correlated with OIP5-AS1 expression in EC cells. Overexpression of VOPP1 ameliorated the negative effects of OIP5-AS1-knockdown on EC9706 and EC109 cells. In conclusion, OIP5-AS1 promoted the proliferation, migration and invasion of EC cells by increasing VOPP1 expression by sponging miR-30a.

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“…Cisplatin has been known as one of the most potential and widely used drugs, which is effective in a variety of solid cancers such as testicular, ovarian, head and neck, bladder, lung, cervical, melanoma, and lymphomas (21)(22)(23)(24)(25). The antimetabolite 5fluorouracil (5-FU), which can inhibit thymidylate synthase, is a widely used antitumor agent (26).…”

Section: Gcda Induces Erk1/2 Phosphorylation Which May Be Involved In Prolonged Survival Of Human Liver Cancer Cellsmentioning

confidence: 99%

Suppressing ERK Pathway Impairs Glycochenodeoxycholate-Mediated Survival and Drug-Resistance in Hepatocellular Carcinoma Cells

Zhou

Wang

et al. 2021

Front. Oncol.

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Glycochenodeoxycholate (GCDA), a toxic component in bile salts, is involved in carcinogenesis of gastrointestinal tumors. The objective of this research was to study the function of ERK1/2 in the GCDA-mediated survival and drug-resistance in hepatocellular carcinoma cells (HCCs). Firstly, extracellular signal-regulated kinase 1/2 (ERK1/2) was detected extensively expressed in liver cancer cells, and silencing ERK1/2 by RNA interference could suppress GCDA-stimulated survival and promote apoptosis. Furthermore, phosphorylation of endogenous ERK1/2 could be potently stimulated by GCDA in combination with enhanced chemoresistance in QGY-7703 hepatocellular carcinoma cells. The GCDA-mediated proliferation and chemoresistance could be impaired by PD98059, which acted as an inhibitor to block the phosphorylation of ERK1/2. Mechanistically, PD98059 was able to potently suppress GCDA-stimulated nuclear aggregation of ERK1/2 and p-ERK1/2, upregulate pro-survival protein Mcl-1 and downregulate pro-apoptotic protein Bim. The results of this study indicated that disruption of ERK1/2 by blocking phosphorylation or nuclear translocation may put forward new methods for solving the problem of GCDA-related proliferation and drug-resistance in liver cancer treatment.

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LncRNA PVT1 accelerates malignant phenotypes of bladder cancer cells by modulating miR-194-5p/BCLAF1 axis as a ceRNA

Cited by 37 publications

References 61 publications

<p>Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer</p>

<p>Apolipoprotein C1 (APOC1): A Novel Diagnostic and Prognostic Biomarker for Cervical Cancer</p>

Long non‑coding RNA OIP5‑AS1 promotes the progression of esophageal cancer by regulating miR‑30a/VOPP1 expression

Suppressing ERK Pathway Impairs Glycochenodeoxycholate-Mediated Survival and Drug-Resistance in Hepatocellular Carcinoma Cells

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