LncRNA SPRY4-IT was concerned with the poor prognosis and contributed to the progression of thyroid cancer

Zhou, Hua‐Cheng; Li, Songwei; Zhou, Xuejun

doi:10.1038/s41417-017-0003-0

Cited by 24 publications

(13 citation statements)

References 30 publications

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“…Of note, a few studies indicated that lncRNAs was associated with cancer progression and could serve as biomarkers for thyroid cancer. For example, lncRNA SPRY4-IT1 was observed to be overexpressed and correlated with poor prognosis in thyroid cancer [21]. In the present study, we demonstrated for the first time that ZFAS1 was overexpressed in thyroid cancer tissues and associated with associated with age, lymph node metastasis, and histological type in thyroid cancer.…”

Section: Discussionsupporting

confidence: 58%

Long Non-Coding RNA ZFAS1 as a Novel Potential Biomarker for Predicting the Prognosis of Thyroid Cancer

Han

Huang

2019

Med Sci Monit

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Background Thyroid cancer is a type of endocrine cancers with rapidly increased incidence. Recent studies have indicated long non-coding RNAs (lncRNAs) played crucial roles in thyroid cancer tumorigenesis and progression. However, the roles of most lncRNAs in thyroid cancer were still unclear. Material/Methods We used TCGA (The Cancer Genome Atlas), GSE50901, GSE29265, and GSE33630 datasets to analyze the expression pattern of ZFAS1 (ZNFX1 antisense RNA 1). The correlation between ZFAS1 and clinicopathological features in thyroid cancer was analyzed. Cell proliferation and cell cycle assays were used to validate the roles of ZFAS1 in thyroid cancer cell lines. DAVID (the database for annotation, visualization and integrated discover) system was used to perform GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis. The starBase datasets and Cytoscape was used to perform ceRNA (competitive endogenous RNA) network. Results We demonstrated ZFAS1 was highly expressed in thyroid cancer compared to normal samples. Moreover, upregulation of ZFAS1 was positively correlated with clinicopathological features and poor prognosis in thyroid cancer. Functional validation showed knockdown of ZFAS1 suppressed cell proliferation and cell cycle in thyroid cancer cells. Bioinformatics analysis showed ZFAS1 was associated with translation, rRNA processing, intra-Golgi vesicle-mediated transport, ribosome, and ubiquitin-mediated proteolysis. Conclusions Our study suggested ZFAS1 could serve as a biomarker for thyroid cancer.

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Section: Discussionsupporting

confidence: 58%

Long Non-Coding RNA ZFAS1 as a Novel Potential Biomarker for Predicting the Prognosis of Thyroid Cancer

Han

Huang

2019

Med Sci Monit

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“…LncRNA HOXA-AS2 facilitates tumorigenesis and progression of PTC by enhancing the Wnt/β-catenin signaling (15) while LncRNA PTCSC3 attenuates drug resistance of ATC to chemotherapeutic drugs by inhibiting the STAT3/INO80 pathway (16). Furthermore, LncRNA SPRY4-IT promotes the progression of TC by targeting the TGF-β/Smad signaling (17). Therefore, identifying and exploring new lncRNAs that regulate the malignancy of TC may uncover potential therapeutic targets for the treatment of TC.…”

Section: Introductionmentioning

confidence: 99%

LncRNA NBR2 Inhibits the Malignancy of Thyroid Cancer, Associated With Enhancing the AMPK Signaling

Yang

Zheng

et al. 2020

Front. Oncol.

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Long non-coding RNA NBR2 is a transcript of the neighbor of BRCA1 gene 2 and can regulate tumor development. However, there is little information on the role of NBR2 in the progression of thyroid cancers (TC). Here, we show that NBR2 expression is down-regulated in TC tissues and associated with histologic subtypes of TC. NBR2 expression was variably reduced in different TC cells. While NBR2 silencing significantly enhanced the malignancy of BCPAP cells by increasing cell proliferation, clonogenicity, wound healing, and invasion as well as tumor growth in vivo, and decreasing spontaneous apoptosis, NBR2 over-expression had opposite effects in BHT101 cells. Furthermore, treatment with A-769662 (a specific AMPK activator), like NBR2 over-expression, significantly attenuated the malignancy of BHT101 cells while treatment with Compound C (a specific AMPK inhibitor) significantly rescued that NBR2reduced malignancy of BHT101 cells. In comparison with non-tumor thyroid epithelial Nthy-ori 3-1 cells, obviously increased GLUT-1 expression, but decreased AMPK and ACC phosphorylation were detected in TC cells. While NBR2 silencing further enhanced GLUT-1 expression and reduced AMPK and ACC phosphorylation as well as the EMT process in BCPAP cells. NBR2 over-expression also had opposite effects in BHT101 cells. Similar patterns of GLUT-1 expression and AMPK and ACC phosphorylation were detected in the different types of xenograft TC tumors in vivo. Therefore, such data indicated that NBR2 acted as a tumor suppressor of thyroid cancers associated with enhancing the AMPK signaling and NBR2 may be a potential biomarker and therapeutic target for thyroid cancers.

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“…SPRY4-IT1 levels were highly positively correlated with tumor size, TNM stage, invasion depth, distant metastasis, and reduced OS (overall survival) and DFS (disease-free survival) in gastric cancer [ 14 ]. Recently, Zhou et al reported that SPRY4-IT was concerned with the poor prognosis and contributed to the progression of thyroid cancer [ 22 ]. These reports suggest that SPRY4-IT1 plays an oncogenic role in human cancers.…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells

et al. 2018

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Accumulating evidence has demonstrated that long non-coding RNAs (lncRNAs) play a critical role in the development of human cancers including pancreatic cancer. Long non-coding RNA SPRY4-IT1 (sprouty4-intron transcript 1) has been reported to play an oncogenic role in various types of human carcinomas. However, the role of SPRY4-IT1 in pancreatic cancer is unclear. The objective of this study was to determine the function of SPRY4-IT1 on proliferation and invasion in pancreatic cancer. In the current study, we dissected the function and mechanism of SPRY4-IT1 by multiple approaches including Real-time RT-PCR, Western blotting analysis, MTT assay, Wound healing assay, Transwell assay, and transfection. We found that down-regulation of SPRY4-IT1 inhibited cell growth and induced cell apoptosis in pancreatic cancer cells. Moreover, SPRY4-IT1 knockdown induced cell cycle arrest at G0/G1 phase. Furthermore, inhibition of SPRY4-IT1 retarded cell migration and invasion in pancreatic cancer cells. Overexpression of SPRY4-IT1 enhanced cell growth and invasion, and inhibited cell apoptosis in pancreatic cancer cells. Mechanistically, suppression of SPRY4-IT1 inhibited the expression of Cdc20 in pancreatic cancer cells. Our findings demonstrated that inhibition of SPRY4-IT1 could be a potential therapeutic approach for the treatment of pancreatic cancer.

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LncRNA SPRY4-IT was concerned with the poor prognosis and contributed to the progression of thyroid cancer

Cited by 24 publications

References 30 publications

Long Non-Coding RNA ZFAS1 as a Novel Potential Biomarker for Predicting the Prognosis of Thyroid Cancer

Long Non-Coding RNA ZFAS1 as a Novel Potential Biomarker for Predicting the Prognosis of Thyroid Cancer

LncRNA NBR2 Inhibits the Malignancy of Thyroid Cancer, Associated With Enhancing the AMPK Signaling

Long non-coding RNA SPRY4-IT1 promotes cell proliferation and invasion by regulation of Cdc20 in pancreatic cancer cells

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