lncRNA XIST knockdown suppresses cell proliferation and promotes apoptosis in diabetic cataracts through the miR‑34a/SMAD2 axis

Wang, Chao; Zhao, Ruipeng; Zhang, Suhong

doi:10.3892/mmr.2021.12523

Cited by 8 publications

(2 citation statements)

References 35 publications

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“…Our study’s functional enrichment results showed no significant enrichment in the X-chromosome, indicating that the ARC pathogenesis may be independent of the X-chromosome. Another study found that Xist lncRNA plays a protective role in diabetic cataracts by promoting cell proliferation and decreasing apoptosis through the miR-34a/SMAD2 ( Wang, Zhao & Zhang, 2022 ). It is unclear in our study whether the Xist up-regulation that was observed was a causative or reactive protective factor in ARC.…”

Section: Discussionmentioning

confidence: 99%

ceRNA network construction and identification of hub genes as novel therapeutic targets for age-related cataracts using bioinformatics

Hong

Sun

et al. 2023

PeerJ

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Background The aim of this study is to investigate the genetic and epigenetic mechanisms involved in the pathogenesis of age-related cataract (ARC). Methods We obtained the transcriptome datafile of th ree ARC samples and three healthy, age-matched samples and used differential expression analyses to identify the differentially expressed genes (DEGs). The differential lncRNA-associated competing endogenous (ceRNA) network, and the protein-protein network (PPI) were constructed using Cytoscape and STRING. Cluster analyses were performed to identify the underlying molecular mechanisms of the hub genes affecting ARC progression. To verify the immune status of the ARC patients, immune-associated analyses were also conducted. Results The PPI network identified the FOXO1 gene as the hub gene with the highest score, as calculated by the Maximal Clique Centrality (MCC) algorithm. The ceRNA network identified lncRNAs H19, XIST, TTTY14, and MEG3 and hub genes FOXO1, NOTCH3, CDK6, SPRY2, and CA2 as playing key roles in regulating the pathogenesis of ARC. Additionally, the identified hub genes showed no significant correlation with an immune response but were highly correlated with cell metabolism, including cysteine, methionine, and galactose. Discussion The findings of this study may provide clues toward ARC pathogenic mechanisms and may be of significance for future therapeutic research.

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Section: Discussionmentioning

confidence: 99%

ceRNA network construction and identification of hub genes as novel therapeutic targets for age-related cataracts using bioinformatics

Hong

Sun

et al. 2023

PeerJ

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“…In conclusion, XIST was identified as a promoter of cell proliferation and an inhibitor of apoptosis in DC, functioning through the regulatory axis of miR‑34a/SMAD2. These findings propose the XIST/miR-34a/SMAD2 axis could serve as a potential novel biomarker for the management of DC (Wang et al 2022a ).…”

Section: Lncrna/mirna/mrna Axis-mediated Apoptosis In Drmentioning

confidence: 98%

Mechanistic and therapeutic perspectives of non-coding RNA-modulated apoptotic signaling in diabetic retinopathy

Wu,

Liu,

Shu

et al. 2024

Cell Biol Toxicol

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Diabetic retinopathy (DR), a significant and vision-endangering complication associated with diabetes mellitus, constitutes a substantial portion of acquired instances of preventable blindness. The progression of DR appears to prominently feature the loss of retinal cells, encompassing neural retinal cells, pericytes, and endothelial cells. Therefore, mitigating the apoptosis of retinal cells in DR could potentially enhance the therapeutic approach for managing the condition by suppressing retinal vascular leakage. Recent advancements have highlighted the crucial regulatory roles played by non-coding RNAs (ncRNAs) in diverse biological processes. Recent advancements have highlighted that non-coding RNAs (ncRNAs), including microRNAs (miRNAs), circular RNAs (circRNAs), and long non-coding RNAs (lncRNAs), act as central regulators in a wide array of biogenesis and biological functions, exerting control over gene expression associated with histogenesis and cellular differentiation within ocular tissues. Abnormal expression and activity of ncRNAs has been linked to the regulation of diverse cellular functions such as apoptosis, and proliferation. This implies a potential involvement of ncRNAs in the development of DR. Notably, ncRNAs and apoptosis exhibit reciprocal regulatory interactions, jointly influencing the destiny of retinal cells. Consequently, a thorough investigation into the complex relationship between apoptosis and ncRNAs is crucial for developing effective therapeutic and preventative strategies for DR. This review provides a fundamental comprehension of the apoptotic signaling pathways associated with DR. It then delves into the mutual relationship between apoptosis and ncRNAs in the context of DR pathogenesis. This study advances our understanding of the pathophysiology of DR and paves the way for the development of novel therapeutic strategies. Graphical Abstract

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oar‐miR‐411a‐5p Promotes Proliferation and Differentiation in Hu Sheep Myoblasts Under Heat Stress by Targeting SMAD2

Lu,

Liu,

2024

Journal Cellular Physiology

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MicroRNAs (miRNAs) are endogenous noncoding RNAs that produce a remarked effect on regulating posttranscriptional gene expression. Our previous study identified a decrease in the expression of oar‐miR‐411a‐5p from umbilical plasma in intrauterine growth restriction (IUGR) Hu lambs subjected to maternal heat stress. In this study, we demonstrated that oar‐miR‐411a‐5p could modulate skeletal muscle development. Overexpression of oar‐miR‐411a‐5p significantly enhanced proliferation and differentiation in heat‐stressed Hu sheep myoblasts while suppressing apoptosis. Conversely, inhibition of oar‐miR‐411a‐5p resulted in opposing effects. Subsequently, RNAhybrid analysis revealed targeted sites between oar‐miR‐411a‐5p and the 3′ untranslated region (UTR) of SMAD2. This suggested that SMAD2 is a direct target gene of oar‐miR‐411a‐5p, as its expression was negatively modulated by oar‐miR‐411a‐5p, a finding corroborated by dual‐luciferase assay and RT‐qPCR. Furthermore, co‐transfection of oar‐miR‐411a‐5p and SMAD2 into Hu sheep myoblasts indicated that oar‐miR‐411a‐5p modulated heat‐stressed myoblast growth by targeting SMAD2. In conclusion, these findings elucidate the function of oar‐miR‐411a‐5p in promoting the development of heat‐stressed Hu sheep myoblasts, thereby enhancing our understanding of how miRNAs influence skeletal muscle growth in heat‐stressed Hu sheep.

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lncRNA XIST knockdown suppresses cell proliferation and promotes apoptosis in diabetic cataracts through the miR‑34a/SMAD2 axis

Cited by 8 publications

References 35 publications

ceRNA network construction and identification of hub genes as novel therapeutic targets for age-related cataracts using bioinformatics

ceRNA network construction and identification of hub genes as novel therapeutic targets for age-related cataracts using bioinformatics

Mechanistic and therapeutic perspectives of non-coding RNA-modulated apoptotic signaling in diabetic retinopathy

oar‐miR‐411a‐5p Promotes Proliferation and Differentiation in Hu Sheep Myoblasts Under Heat Stress by Targeting SMAD2

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