LncRNAs act as prognostic biomarkers in bladder carcinoma: A meta-analysis

Zhong, Yucheng; Zhang, Yeshen; Li, Huan; Ma, Wenzhi

doi:10.1016/j.heliyon.2019.e02785

Cited by 4 publications

(3 citation statements)

References 31 publications

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“…[19][20][21] Meanwhile, meta-analysis of several studies revealed that the expression levels of lncRNAs are associated with the prognosis of BC. 22 Increasing numbers of studies have reported that lncRNAs can serve as competing endogenous RNAs (ceRNAs) to bind miRNAs, consequently regulating the expression levels of downstream genes. 23,24 Some lncRNAs have been identified as molecular biomarkers for BC patients; however, novel correlated lncRNAs are urgently needed.…”

Section: Discussionmentioning

confidence: 99%

<p>LncRNA RNF144A-AS1 Promotes Bladder Cancer Progression via RNF144A-AS1/miR-455-5p/SOX11 Axis</p>

Shang

Jia

et al. 2020

OTT

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Background Bladder cancer (BC) is the most commonly occurring malignant tumor of the urinary system worldwide. Long non-coding RNAs (lncRNAs), including lncRNA RNF144A-AS1 (RNF144A-AS1), perform an oncogenic role in BC progression. However, how RNF144A-AS1 is regulated in BC has not been fully investigated, and its role in BC is mostly obscure. In this study, we explore its role in BC progression. Materials and Methods The expression level of RNF144A-AS1 in BC tissues was explored via bioinformatics analysis and quantitative real-time PCR (qRT-PCR). We used RNF144A-AS1 siRNA (si-RNF144A-AS1) to inhibit the RNF144A-AS1 level in BC cell lines (J82 and 5637 cells). A series of experimental studies in vitro (CCK-8 assay, colony formation assay and Transwell assay) was performed to explore the role of si-RNF144A-AS1 on the proliferation, migration and invasion of J82 and 5637 cells. A BC xenograft model was established, and the effect of si-RNF144A-AS1 on xenograft growth was explored in vivo. The interactions among RNF144A-AS1, miR-455-5p and SOX11 were predicted by bioinformatics miRanda and Targetscan database, and verified by the luciferase reporter assay and RNA pull-down assay. Finally, miR-455-5p inhibitor and si-RNF144A-AS1 were cotransfected into J82 and 5637 cells. Results RNF144A-AS1 is overexpressed in BC tumors and cells, and its overexpression is correlated with poor prognosis. Knockdown of RNF144A-AS1 markedly suppressed the proliferation, migration and invasion of J82 and 5637 cells and significantly inhibited xenograft growth in nude mice, compared to si-NC. We found that RNF144A-AS1 serves as a sponge for miR-455-5p. Furthermore, a binding site of miR-455-5p was found in 3ʹ UTR of SOX11 gene, and overexpression of miR-455-5p suppressed SOX11 levels. RNF144A-AS1 knockdown markedly decreased SOX11 expression levels, while miR-455-5p inhibitor restored this repressive effect. Restoration of SOX11 could reverse this repressive effect of RNF144A-AS1 on cell proliferation, migration and invasion abilities. Conclusion Overall, our findings underline the critical role of RNF144A-AS1 in BC development, and our study reveals for the first time that RNF144A-AS1 promotes BC progression via the RNF144A-AS1/miR-455-5p/SOX11 axis.

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Section: Discussionmentioning

confidence: 99%

<p>LncRNA RNF144A-AS1 Promotes Bladder Cancer Progression via RNF144A-AS1/miR-455-5p/SOX11 Axis</p>

Shang

Jia

et al. 2020

OTT

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“…Molecular markers for early detection could be the most effective method of BlCa prevention, however the molecular mechanisms underlying BlCa progression are still not well characterized. Numerous publications have suggested that lncRNAs, such as UCA-1, MALAT1, PANDAR and so on, play key roles in development and progression of bladder cancer [4]. Nevertheless, their clinical use is still limited and none is yet validated or widely used in the clinical practice [5].…”

Section: Introductionmentioning

confidence: 99%

Subcellular Localization of uc.8+ as a Prognostic Biomarker in Bladder Cancer Tissue

Terreri

Mancinelli

Ferro

et al. 2021

Cancers

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Non-coding RNA transcripts originating from Ultraconserved Regions (UCRs) have tissue-specific expression and play relevant roles in the pathophysiology of multiple cancer types. Among them, we recently identified and characterized the ultra-conserved-transcript-8+ (uc.8+), whose levels correlate with grading and staging of bladder cancer. Here, to validate uc.8+ as a potential biomarker in bladder cancer, we assessed its expression and subcellular localization by using tissue microarray on 73 human bladder cancer specimens. We quantified uc.8+ by in-situ hybridization and correlated its expression levels with clinical characteristics and patient survival. The analysis of subcellular localization indicated the simultaneous presence of uc.8+ in the cytoplasm and nucleus of cells from the Low-Grade group, whereas a prevalent cytoplasmic localization was observed in samples from the High-Grade group, supporting the hypothesis of uc.8+ nuclear-to-cytoplasmic translocation in most malignant tumor forms. Moreover, analysis of uc.8+ expression and subcellular localization in tumor-surrounding stroma revealed a marked down-regulation of uc.8+ levels compared to the paired (adjacent) tumor region. Finally, deep machine-learning approaches identified nucleotide sequences associated with uc.8+ localization in nucleus and/or cytoplasm, allowing to predict possible RNA binding proteins associated with uc.8+, recognizing also sequences involved in mRNA cytoplasm-translocation. Our model suggests uc.8+ subcellular localization as a potential prognostic biomarker for bladder cancer.

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“…Bcl-2 overexpression in bladder cancer has been also reported to play an important role in cisplatin resistance ( Cho et al, 2006 ). The very first study by Zhong et al has shown atypical/aberrant expression of ncRNAs in bladder cancer might be exploited as bladder cancer biomarkers ( Zhong et al, 2019 ).…”

Section: Bladder Cancer and Non-coding Rnas (Ncrnas)mentioning

confidence: 99%

Non-coding RNA and autophagy: Finding novel ways to improve the diagnostic management of bladder cancer

2023

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Major fraction of the human genome is transcribed in to the RNA but is not translated in to any specific functional protein. These transcribed but not translated RNA molecules are called as non-coding RNA (ncRNA). There are thousands of different non-coding RNAs present inside the cells, each regulating different cellular pathway/pathways. Over the last few decades non-coding RNAs have been found to be involved in various diseases including cancer. Non-coding RNAs are reported to function both as tumor enhancer and/or tumor suppressor in almost each type of cancer. Urothelial carcinoma of the urinary bladder is the second most common urogenital malignancy in the world. Over the last few decades, non-coding RNAs were demonstrated to be linked with bladder cancer progression by modulating different signalling pathways and cellular processes such as autophagy, metastasis, drug resistance and tumor proliferation. Due to the heterogeneity of bladder cancer cells more in-depth molecular characterization is needed to identify new diagnostic and treatment options. This review emphasizes the current findings on non-coding RNAs and their relationship with various oncological processes such as autophagy, and their applicability to the pathophysiology of bladder cancer. This may offer an understanding of evolving non-coding RNA-targeted diagnostic tools and new therapeutic approaches for bladder cancer management in the future.

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LncRNAs act as prognostic biomarkers in bladder carcinoma: A meta-analysis

Cited by 4 publications

References 31 publications

<p>LncRNA RNF144A-AS1 Promotes Bladder Cancer Progression via RNF144A-AS1/miR-455-5p/SOX11 Axis</p>

<p>LncRNA RNF144A-AS1 Promotes Bladder Cancer Progression via RNF144A-AS1/miR-455-5p/SOX11 Axis</p>

Subcellular Localization of uc.8+ as a Prognostic Biomarker in Bladder Cancer Tissue

Non-coding RNA and autophagy: Finding novel ways to improve the diagnostic management of bladder cancer

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