LncRNAs ENST 00000499459 and TCONS_00004989 enhance asthma progression in children with house dust mite-induced allergic asthma

Abstract

Background Long noncoding RNAs (lncRNAs) have been extensively reported to play critical roles in disease pathogenesis, especially in the field of cancer. However, there has been limited research on the role of lncRNAs in the pathogenesis of pediatric allergic asthma. Methods High-throughput sequencing analysis was performed to identify differentially expressed mRNAs and lncRNAs in peripheral blood mononuclear cells (PBMCs) from 3 children with allergic asthma and 3 matched healthy controls. Bioinformatics analysis was used to select candidate lncRNAs and mRNAs that may be involved in the pathogenesis of asthma. Candidate lncRNAs were used for validation in a larger sample of asthma patients and healthy controls. Finally, lncRNAs and molecular pathways associated with the pathogenesis of allergic asthma were identified by competing endogenous RNA (ceRNA) analysis. Results Five differentially expressed lncRNAs (DE lncRNAs) were identified after high-throughput sequencing and verified by real-time PCR. LncRNAs ENST0000631797, TCONS_00004989 and ENST00000499459 were verified to have significant differences in expression among the PBMC samples from 15 AAS patients and 15 healthy controls. ENST00000499459/DIXDC1 was finally identified as a marker molecule of allergic asthma after comprehensive ceRNA network analysis. Conclusion ENST00000499459 and TCONS_00004989 are potential biomarkers in children with house dust mite-induced allergic asthma.