Local Actin Polymerization and Dynamin Recruitment in SV40-Induced Internalization of Caveolae

Pelkmans, Lucas; Püntener, Daniel; Helenius, Ari

doi:10.1126/science.1069784

Cited by 645 publications

(610 citation statements)

References 31 publications

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“…The clusters of vesicles, each vesicle loaded with a single virion, fused with irregularly shaped vesiculartubular structures ( Figure 2). These latter structures were morphologically consistent with the descriptions of caveosomes (9,10,12). In rare instances there were recognizable changes in the cellular cytoskeleton with aggregation of intermediate and thin filaments in the adjacent cytosol.…”

Section: Virions Associated With the Surface Cytoplasmic Membranesupporting

confidence: 87%

“…This virus is internalized in non-clathrin-coated vesicles called caveolae. From caveolae the virions reach the nucleus through an intermediate vesicular and tubular system (9)(10)(11)(12)(13). This is in contrast to many other viruses that enter the host cells by the usual endocytic pathway.…”

Section: Introductionmentioning

confidence: 99%

“…The latter is a constitutive pathway that utilizes clathrin-coated vesicles targeted to the endosomal/lysosomal system. In these organelles the viruses are disassembled through acid-activated hydrolytic enzymes (10,12). Disassembly of SV40, however, is not dependent on low pH (14).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

BK Polyoma Virus Allograft Nephropathy: Ultrastructural Features from Viral Cell Entry to Lysis

Drachenberg¹,

Papadimitriou²,

Wali³

et al. 2003

American Journal of Transplantation

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BK virions must enter the host cell and target their genome to the nucleus in order to complete their life cycle. The mechanisms by which the virions accomplish these tasks are not known. In this morphological study we found that BK virions localized beneath the host cell cytoplasmic membrane in 60-70-nm, smooth (non-coated) monopinocytotic vesicles similar to, or consistent with, caveolae. In the cytoplasm, the monopinocytotic vesicles carrying virions appeared to fuse with a system of smooth, vesicles and tubules that communicated with the rough endoplasmic reticulum and was continuous with the Golgi system. Membrane-bound single virions and large tubuloreticular complexes loaded with virions accumulated in paranuclear locations. Occasional nuclei displayed virions within the perinuclear cisterna in association to the perinuclear viral accumulations. Tubular cells with mature productive infection had large nuclei, distended by daughter virions, whereas they lacked significant numbers of cytoplasmic virions. In addition to virally induced cell necrosis, there was extensive tubular cell damage (apoptosis and necrosis) in morphologically non-infected tubules. The observed ultrastructural interactions between the BK virions and host cells are remarkably similar to viral cell entry and nuclear targeting described for SV40 virus.

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Section: Virions Associated With the Surface Cytoplasmic Membranesupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

BK Polyoma Virus Allograft Nephropathy: Ultrastructural Features from Viral Cell Entry to Lysis

Drachenberg¹,

Papadimitriou²,

Wali³

et al. 2003

American Journal of Transplantation

View full text Add to dashboard Cite

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“…2). Most SV40 co-localizes with caveolae, activating tyrosin kinases and inducing rearrangement of actin stress fibres to form actin tails on virusloaded caveolae 21 (FIG. 2).…”

Section: Viral Entrymentioning

confidence: 99%

Virus trafficking – learning from single-virus tracking

2007

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What could be a better way to study virus trafficking than 'miniaturizing oneself' and 'taking a ride with the virus particle' on its journey into the cell? Single-virus tracking in living cells potentially provides us with the means to visualize the virus journey. This approach allows us to follow the fate of individual virus particles and monitor dynamic interactions between viruses and cellular structures, revealing previously unobservable infection steps. The entry, trafficking and egress mechanisms of various animal viruses have been elucidated using this method. The combination of single-virus trafficking with systems approaches and state-of-the-art imaging technologies should prove exciting in the future.

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“…Viruses such as SV40 take a similar approach in disrupting the actin cytoskeleton through the activation of tyrosine kinases to aid in their movement through the cytoplasm. 34 Thus, it would seem likely that actin depolymerization plays some role in the stretch-induced increase in gene expression. However, it would seem that simple depolymerization alone is not sufficient for enhanced expression as shown by the fact that the addition of latrunculin B does not increase gene transfer and expression in static cells to the same level that stretching the cells does.…”

Section: The Percent Of Apoptotic Cells (7sd) Is Shownmentioning

confidence: 99%

Cyclic stretch-induced reorganization of the cytoskeleton and its role in enhanced gene transfer

et al. 2006

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Cyclic stretch is known to alter a number of cellular and subcellular processes, including those involved in nonviral gene delivery. We have previously shown that moderate equibiaxial cyclic stretch (10% change in basement membrane area, 0.5 Hz, 50% duty cycle) of human pulmonary A549 cells enhances gene transfer and expression of reporter plasmid DNA in vitro, and that this phenomena may be due to alterations in cytoplasmic trafficking. Although the path by which plasmid DNA travels through the cytoplasm toward the nucleus is not well understood, the cytoskeleton and the constituents of the cytoplasm are known to significantly hinder macromolecular diffusion. Using biochemical techniques and immunofluorescence microscopy, we show that both the microfilament and microtubule networks are significantly reorganized by equibiaxial cyclic stretch. Prevention of this reorganization through the use of cytoskeletal stabilizing compounds mitigates the stretchinduced increase in gene expression, however, depolymerization in the absence of stretch is not sufficient to increase gene expression. These results suggest that cytoskeletal reorganization plays an important role in stretch-induced gene transfer and expression. Gene Therapy (2006) 13, 725-731.

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Local Actin Polymerization and Dynamin Recruitment in SV40-Induced Internalization of Caveolae

Cited by 645 publications

References 31 publications

BK Polyoma Virus Allograft Nephropathy: Ultrastructural Features from Viral Cell Entry to Lysis

BK Polyoma Virus Allograft Nephropathy: Ultrastructural Features from Viral Cell Entry to Lysis

Virus trafficking – learning from single-virus tracking

Cyclic stretch-induced reorganization of the cytoskeleton and its role in enhanced gene transfer

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