Local cytoplasmic calcium gradients in living mitotic cells

Keith, Charles H.; Ratan, Rajiv R.; Maxfield, Frederick R.; Bajer, A.; Shelanski, Michael L.

doi:10.1038/316848a0

Cited by 155 publications

(65 citation statements)

References 27 publications

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“…This idea was supported by early reports that [Ca 2+ ] is elevated at spindle poles before anaphase in PTK and endosperm cells (Keith et al, 1985;Ratan et al, 1986). More recently, using confocal imaging and ratiometric Ca 2+ indicators, localized Ca 2+ increases were detected in the perinuclear region just before NEBD in sea-urchin embryos (Wilding et al, 1996).…”

Section: Casupporting

confidence: 69%

An increase in [Ca2+]i is sufficient but not necessary for driving mitosis in early mouse embryos

FitzHarris

Larman

Richards

et al. 2005

Journal of Cell Science

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An increase in intracellular Ca2+ concentration ([Ca2+]i) has been shown to drive sea-urchin embryos and some fibroblasts through nuclear-envelope breakdown (NEBD) and the metaphase-to-anaphase transition. Mitotic Ca2+ transients can be pan-cellular global events or localized to the perinuclear region. It is not known whether Ca2+ is a universal regulator of mitosis or whether its role is confined to specific cell types. To test the hypothesis that Ca2+ is a universal regulator of mitosis, we have investigated the role of Ca2+ in mitosis in one-cell mouse embryos. Fertilized embryos generate Ca2+ transients during the first mitotic division. Imposing a Ca2+ transient by photorelease of inositol (1,4,5)-trisphosphate [Ins(1,4,5)P3] resulted in acceleration of mitosis entry, suggesting that a [Ca2+]i increase is capable of triggering mitosis. Mitotic Ca2+ transients were inhibited using three independent approaches: injection of intracellular Ca2+ buffers; downregulation of Ins(1,4,5)P3 receptors; and removal of extracellular Ca2+. None of the interventions had any effects on the timing of NEBD or cytokinesis. The possibility that NEBD is driven by localized perinuclear Ca2+ transients was examined using two-photon microscopy but no Ca2+-dependent increases in fluorescence were found to precede NEBD. Finally, the second mitotic division took place in the absence of any detectable [Ca2+]i increase. Thus, although an induced [Ca2+]i increase can accelerate mitosis entry, neither cytosolic nor perinuclear [Ca2+] increases appear to be necessary for progression through mitosis in mouse embryos.

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Section: Casupporting

confidence: 69%

An increase in [Ca2+]i is sufficient but not necessary for driving mitosis in early mouse embryos

FitzHarris

Larman

Richards

et al. 2005

Journal of Cell Science

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“…Ion channels are likely to be involved in cellular development; Norvac & Bentrup (1972) found the regenerative pole of Acetabularia corresponded to the site of action potentials, and this may be related to the higher levels of Ca^^ found in growing tips (Brownlee & Wood, 1986;Nobiling & Reiss, 1987) and at the spindle poles of mitotic cells (Keith et aL, 1985). Whole-cell free Ca^^ may rise during anaphase (Hepler & Callahan, 1987), which might be related to plasma membrane Ca"'ĉ hannel activity.…”

Section: Ion Channels and Plant Developmentmentioning

confidence: 99%

Tansley Review No. 21 Plant ion channels: whole‐cell and single channel studies

Tester¹

1990

New Phytologist

301

166

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SUMMARY Ion channels are proteins which catalyse rapid, passive, electrogenic uniport of ions through pores spanning an otherwise poorly permeable lipid bilayer. Among other processes, fluxes through ion channels are responsible for action potentials – large, transient changes in membrane potential which have been known of in plants for over 100 years. Much disparate information on ion channels in plant cells has accumulated over the past few years. In an attempt to synthesize these data, the properties of at least 18 different ion channels are collated in this review. Channels are initially classified according to ion selectivity (Ca2+, Cl−, K+ and H+); then gating characteristics (i.e. control of opening and closing), unitary conductance and pharmacology are used to distinguish further different sub‐types of channels. To provide a background for this overview, the fundamental properties which define ion channels in animal cells, namely conduction, selectivity and gating, are described. Appropriate techniques for the study of ion channels are also assessed. The review concludes with a discussion on the role of ion channels in plant cells, although any comment on functions beyond turgor regulation and general statements about signalling remains largely speculative. The study of ion channels in plant cells is still at an early stage and it is hoped that this review will provide a framework upon which further work in both algae and vascular plants can be based.

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“…Such a procedure can be used not only in cell suspensions, but also in single cells individually in a fluorescent microscope. The investigation with Fura-2 can further be pursued at the subcellular level through the use of imaging techniques (4,12,19,20). In the present study a change of [Ca2+]i of hepatocytes following the addition of DMSO is examined using a digital imaging microscope, since [Ca2+]i is related to the polymerization and depolymerization of actin in nonmuscle cells (5,7,15,24 division to exclude non-cellular regions from the determination of the ratio image.…”

Section: Isommentioning

confidence: 99%

Effect of dimethyl sulfoxide on cytosolic ionized calcium concentration and cytoskeletal organization of hepatocytes in a primary culture.

Yamamoto

1989

Cell Struct. Funct.

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ABSTRACT. The addition of dimethyl sulfoxide (DMSO) to a chemically defined, serum free medium prolonged hepatocytes survival in primary culture. DMSO exposure had a remarkable effect on morphological change and F-actin filaments distribution of hepatocytes. When hepatocytes were cultured in a medium containing 2% DMSO, the cells showed a compact and cubical shape and intracellular F-actin filaments were mainly observed in a ring-like fashion around the intercellular space. After exposure to DMSO, fibronectin fibers in the interspace between cell and substratum were not apparent.Exposing the hepatocytes to DMSO also caused a sharp increase in cytosolic free ionized calcium ([Ca2+]i). The initial increase in [Ca2+]i following the addition of DMSO was not attenuated by the chelation of extracellular Ca2+ with EGTA. The Ca2+ signal in the absence of extracellular Ca2+ was transient and returned to the basal levels within 1-2 min, while it was maintained at a high steady state in the presence of extracellular Ca2+ . These results suggest that DMSO may be able to increase [Ca2+]i by two mechanisms, by the release of the ion from intracellular pools and, by the stimulation of influx across the plasma membrane.The increase in [Ca2]i induced by DMSO treatment may play a role in prolonging hepatocyte survival in culture, since [Ca2+]i is one of the most important dynamic second messengers in various cellular metabolic processes.

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Local cytoplasmic calcium gradients in living mitotic cells

Cited by 155 publications

References 27 publications

An increase in [Ca2+]i is sufficient but not necessary for driving mitosis in early mouse embryos

An increase in [Ca2+]i is sufficient but not necessary for driving mitosis in early mouse embryos

Tansley Review No. 21 Plant ion channels: whole‐cell and single channel studies

Effect of dimethyl sulfoxide on cytosolic ionized calcium concentration and cytoskeletal organization of hepatocytes in a primary culture.

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