Local inflammatory events induced by Bothrops atrox snake venom and the release of distinct classes of inflammatory mediators

Moreira, Vanessa; Dos-Santos, Maria Cristina; Nascimento, Neide Galvão; Silva, Henrique Borges da; Fernandes, C.M.; Lima, Maria Regina D'Império; Teixeira, Catarina

doi:10.1016/j.toxicon.2012.03.004

Cited by 78 publications

(58 citation statements)

References 69 publications

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“…These venom components can be classified into procoagulants, anticoagulants, fibrinolytic toxins, vessel wall interactive toxins, toxins that affect platelet activity and plasma protein activators . These not only destroy tissues, but can also evoke a significant inflammatory response through cytokine release in the victim . In viperine (adders) and crotaline (rattlesnakes) species, inflammation is a major characteristic of envenoming and inflammation and coagulation are known to inadvertently activate each other …”

Section: Introductionmentioning

confidence: 99%

Hemostatic analysis of dogs naturally envenomed by the African puffadder (Bitis arietans) and snouted cobra (Naja annulifera)

Nagel

Schoeman

Thompson³

et al. 2014

J Vet Emergen Crit Care

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Objective -To investigate hemostatic changes in dogs envenomed by cytotoxic (African puffadder) and neurotoxic snakes (snouted cobra) using thromboelastography (TEG) and plasma-based coagulation assays. Design -Prospective observational clinical study. Setting -University teaching hospital. Animals -Eighteen client-owned dogs; 9 envenomed by African puffadder (Bitis arietans) and 9 by snouted cobra (Naja annulifera). Ten healthy dogs served as controls. Interventions -None. Measurements and Main Results -Blood was collected at presentation and 24 hours post envenomation. Platelet count, TEG, prothrombin time, activated partial thromboplastin time (aPTT), antithrombin activity, and fibrinogen (Fib) and C-reactive protein (CRP) concentrations were measured. Outcomes were analyzed using linear mixed models at 5% significance. At presentation, R time was significantly prolonged in the puffadder group compared to the cobra (P = 0.01) and control groups (P = 0.05). Platelet count was significantly lower in the puffadder compared to the cobra (P = 0.04) and control groups (P = 0.001), respectively. Antithrombin activity was significantly decreased in the puffadder (P = 0.002) and cobra groups (P = 0.004) compared to the control group. Both prothrombin time and activated partial thromboplastin time were significantly prolonged in the cobra group compared to the control group (P = 0.03 for both). The TEG variables, maximum amplitude (MA) and G, were significantly increased 24 hours post envenomation in the puffadder group compared to their values at presentation (P = 0.05 for both). Fib and CRP concentrations were significantly increased 24 hours post envenomation in both snake-envenomed groups. Conclusions -Prolonged clot initiation was a common feature in puffadder-envenomed dogs at presentation and this was likely venom induced. Snouted cobra-envenomed dogs were normo-to hypercoagulable at presentation. Dogs from both puffadder and cobra groups progressed to a more hypercoagulable by 24 hours post envenomation, most likely due to marked inflammation as indicated by the increased Fib and CRP concentrations. TEG proved a sensitive tool for detecting abnormal hemostasis in snake-envenomed dogs.

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Section: Introductionmentioning

confidence: 99%

Hemostatic analysis of dogs naturally envenomed by the African puffadder (Bitis arietans) and snouted cobra (Naja annulifera)

Nagel

Schoeman

Thompson³

et al. 2014

J Vet Emergen Crit Care

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show abstract

“…In addition, venom has been shown to have pro- and anti-inflammatory properties and has been shown to regulate the expression of inflammatory mediators such as IL-1β, IL-6, IL-10, IL-22, TNF-α, PGD2, PGE2, and TXA2 [18,19,21,41,42,43,44]. In this study, we constructed and screened an H. cyanocinctus venom gland T7 phage display library and discovered H-TL1, a natural peptide with antagonizing effects against TNF-α.…”

Section: Discussionmentioning

confidence: 99%

Hydrostatin-TL1, an Anti-Inflammatory Active Peptide from the Venom Gland of Hydrophis cyanocinctus in the South China Sea

Wang

Huang

Liu

et al. 2016

IJMS

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Tumor necrosis factor (TNF)-α is a pleiotropic cytokine with intense pro-inflammatory and immunomodulatory properties, and anti-TNF-α biologics are effective therapies for various inflammatory diseases such as inflammatory bowel disease (IBD) and sepsis. Snake venom, as a traditional Chinese medicine, has been used in the treatment of inflammatory diseases in China for centuries. In this research, we constructed a venom gland T7 phage display library of the sea snake Hydrophis cyanocinctus to screen bioactive compounds that antagonize TNF-α and identified a novel nine-amino-acid peptide, termed hydrostatin-TL1 (H-TL1). In enzyme-linked immunosorbent assay (ELISA) and surface plasmon resonance (SPR) analyses, H-TL1 inhibited the interaction between TNF-α and TNF receptor 1 (TNFR1). Further, H-TL1 attenuated the cytotoxicity of TNF-α in L929 cells as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. H-TL1 also decreased the mRNA expression of TNF-α/TNFR1 downstream targets and suppressed the phosphorylation of well-characterized proteins of downstream signal transduction pathways in HEK-293 cells. In vivo data demonstrated that H-TL1 protects animals against dextran sodium sulfate (DSS)-induced acute colitis and lipopolysaccharide (LPS)-induced acute shock. Given its significant anti-inflammatory activity in vitro and in vivo, H-TL1 is a potential peptide for the development of new agents to treat TNF-α-associated inflammatory diseases.

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“…Bothrops atrox venom, for example, was shown to increase the vascular permeability in mice, inducing leukocyte influx between 1 and 8 h after i.p. injection and releasing important inflammatory mediators such as the eicosanoids PGD 2 , PGE 2 , TXA 2 , LTB 4 , the cytokines TNF-α, IL-6, IL-10, and the chemokine CCL-2 [52]. These inflammatory effects are most commonly related to phospholipases A 2 and metalloproteinases [72,73], although other classes of enzymes may also contribute to these responses [49].…”

Section: Discussionmentioning

confidence: 99%

Inflammatory mediators involved in the paw edema and hyperalgesia induced by Batroxase, a metalloproteinase isolated from Bothrops atrox snake venom

Toni

Menaldo

Cintra

et al. 2015

International Immunopharmacology

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Local inflammatory events induced by Bothrops atrox snake venom and the release of distinct classes of inflammatory mediators

Cited by 78 publications

References 69 publications

Hemostatic analysis of dogs naturally envenomed by the African puffadder (Bitis arietans) and snouted cobra (Naja annulifera)

Hemostatic analysis of dogs naturally envenomed by the African puffadder (Bitis arietans) and snouted cobra (Naja annulifera)

Hydrostatin-TL1, an Anti-Inflammatory Active Peptide from the Venom Gland of Hydrophis cyanocinctus in the South China Sea

Inflammatory mediators involved in the paw edema and hyperalgesia induced by Batroxase, a metalloproteinase isolated from Bothrops atrox snake venom

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