Local strains Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1 as a reducing and detoxifying agents for deoxynivalenol

Abstract

Aims: Deoxynivalenol is a type B trichothecene produced by Fusarium graminearum that can cause serious health problems in human and livestock. The present study aimed to reduce and detoxify deoxynivalenol using a local strain Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1. Methodology and results: Corn as solid substrate artificially inoculated with F. graminearum bio 163252 to produce deoxynivalenol. Deoxynivalenol contaminated corn then inoculated with A. oryzae KKB4 and R. oryzae KP1R1. During fermentation, a decrease in deoxynivalenol levels is analyzed including loss of dry matter and glucosamine content. Deoxynivalenol was extracted from the substrate by solid phase extraction and quantified using high-performance liquid chromatography. The reduction of deoxynivalenol by A. oryzae KKB4 and R. oryzae KP1R1 were 65.91% and 56.82%, respectively after ten days of fermentation. Toxicity analysis revealed that residues of deoxynivalenol were not toxic to growth of Saccharomyces cerevisiae cells. Conclusion, significance and impact of study: Local strains A. oryzae KKB4 and R. oryzae KP1R1 were able to reduce and detoxify deoxynivalenol in solid substrates. This study provides supporting data to control mycotoxin that is critical for food and feed safety.

Deoxynivalenol in food and feed: Recent advances in decontamination strategies

“…Aspergillus oryzae KKB4 and Rhizopus oryzae KP1R1 were inoculated into DON-contaminated corn and fermented for 10 days, which resulted in DON reduction by 65.91% and 56.82%, respectively. Toxicity analysis showed that the degradation products did not affect the growth of Saccharomyces cerevisiae ( Arifin et al, 2019 ). Moreover, the DON degradation percentage in corn medium was as high as 92% after culture of Aspergillus oryzae (5509NRRL) for 21 days ( Tran and Smith, 2014 ).…”

Global distribution, toxicity to humans and animals, biodegradation, and nutritional mitigation of deoxynivalenol: A review

“…Arifin et al. (2019) showed that local strains A. oryzae KKB4 and R. oryzae KP1R1 (0.1% rough powder) could detoxify DON (500 ng/mL) in corn, and the reduction rate of DON was 65.91% and 56.82%, respectively, after 10 days of fermentation.…”

“…Tian, Tan, Liu, Yan, et al (2016) indicated that Trichoderma strains possess a selfprotection mechanism as plants to detoxify DON (57 μg/g) into D3G when competing with F. graminearum. Arifin et al (2019) showed that local strains A. oryzae KKB4 and R. oryzae KP1R1 (0.1% rough powder) could detoxify DON (500 ng/mL) in corn, and the reduction rate of DON was 65.91% and 56.82%, respectively, after 10 days of fermentation.…”