Local therapy of herpes simplex with dried extract from Melissa officinalis

Wölbling, R.H.; Leonhardt, K.

doi:10.1016/s0944-7113(11)80019-x

Cited by 78 publications

(47 citation statements)

References 6 publications

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“…These plants have been widely used in traditional medicine to treat a variety of infectious diseases and cancer [5] and represent an abundant source of new bioactive secondary metabolites. Melissa officinalis (lemon balm) is a member of the Lamiaceae family and plants of this family are well known in antiviral phytotherapy [6,7]. Extracts from plants of the Lamiaceae family have been described for antioxidant [8] and antibacterial effects [9,10] which have been linked to their polyphenolic composition.…”

Section: Introductionmentioning

confidence: 99%

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Astani

Reichling²,

Schnitzler³

2012

Chemotherapy

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Background: Extracts and essential oils of medicinal plants are increasingly of interest as novel drugs for antiherpetic agents, since the herpes simplex virus (HSV) might develop resistance to commonly used antiviral drugs. Methods: An aqueous extract of Melissa officinalis as well as phenolic extract compounds, i.e. caffeic acid, p-coumaric acid and rosmarinic acid were examined for their antiviral activity against herpes simplex virus type 1 (HSV-1) in vitro. Results: When drugs were added to HSV-1-infected cells, no antiviral effect was observed as determined by plaque reduction assay and analysis of expression of viral protein ICP0. However, the Melissa extract demonstrated a high virucidal activity against HSV-1, even at very low concentrations of 1.5 µg/ml, whereas similar results for phenolic compounds were only achieved at 100 times higher concentrations. Besides the virucidal activity, the Melissa extract and rosmarinic acid inhibited HSV-1 attachment to host cells in a dose-dependent manner. These results indicate that rosmarinic acid was the main contributor to the antiviral activity of Melissa extract. However, the selectivity index of Melissa extract of 875 against HSV is superior to the selectivity indices of single constituents. Conclusion: Melissa extract exhibits low toxicity, is virucidal and affects HSV-1 attachment to host cells in vitro.

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Section: Introductionmentioning

confidence: 99%

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Astani

Reichling²,

Schnitzler³

2012

Chemotherapy

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“…Antibacterial effects of rosemary essential oil [5] as well as rosemary extract [6] against Propionibacterium acnes infections have also been reported and linked to the plants' phenolic compositions. Members of the Lamiaceae family are used in traditional as well as in complementary medicine, especially in phytotherapy [7,8]. Rosemary essential oil might be used in drug-resistant infections as reported previously [9].…”

Section: Introductionmentioning

confidence: 99%

Impact of Ethanolic Lamiaceae Extracts on Herpesvirus Infectivity in Cell Culture

Reichling

Nolkemper²,

Stintzing³

et al. 2008

Complement Med Res

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Background: Extracts of medicinal plants are increasingly of interest as novel drugs for antimicrobial and antiviral agents, since microorganisms might develop resistance to commonly used antimicrobial or antiviral agents. Materials and Methods: Ethanolic extracts from Lamiaceae plants prunella, peppermint, rosemary and thyme were phytochemically characterised. The inhibitory activity of four 20% ethanolic plant extracts and four 80% ethanolic extracts against herpes simplex virus (HSV) strains was tested in cell culture. Results: Rosmarinic acid, a typical compound in Lamiaceae species, was identified in the extracts except for thyme 20% ethanolic extract. In addition, some other phenolic compounds such as apigenin- and luteolin-derivatives were identified in different amounts. All extracts exhibited high and concentration-dependent levels of antiviral activity against free acyclovir-sensitive and acyclovir-resistant HSV-1 strains with 50% inhibitory concentrations of 0.05–0.82 μg/ml. Mechanistically, exposure of free virions as well as host cells to prunella and peppermint 80% ethanolic extracts at maximum non-cytotoxic concentrations prior to infection reduced plaque formation drastically. Thus, both extracts revealed a dual mode of action similar to aqueous lemon balm extracts. Conclusions: Since infectivity of acyclovir-susceptible and acyclovir-resistant HSV strains was significantly reduced with Lamiaceae extracts, the results obtained indicate that ethanolic plant extracts affected herpesvirus prior to and during adsorption and in a different way than acyclovir. Based on its dual mode of action, e.g. antiviral effect against free virions and blocking virus attachment to host cells, prunella and peppermint 80% ethanolic extracts are promising antiviral agents in recurrent herpes labialis for topical therapeutic applications.

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“…Investigaciones sobre la Melissa officinalis comparan el efecto antiviral en herpes simple con placebo para la evaluación del tratamiento estableciendo la eficacia de extracto de planta para la remisión del evento (Wölbling & Leonhardt, 1994). Al comparar el mismo efecto con un gold estándar, como el Aciclovir tó-pico al 5% de uso tópico usado en el estudio, se mantiene la eficacia evaluando el número de días con lesiones, estableciendo un primer ciclo que homologa el efecto farmacológico del Aciclovir tópico al 5%, y un segundo ciclo en el cual el Melissa officinalis disminuye el total de días (Sepúlveda Tabache et al, 2003).…”

Section: Discussionunclassified

Estudio Comparativo entre Terapia con Melissa officinalis vs. Tratamiento Convencional con Aciclovir Tópico al 5% para Lesiones Herpéticas Tipo 1

2014

Int. J. Odontostomat.

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entre terapia con Melissa officinalis vs. tratamiento convencional con aciclovir tópico al 5% para lesiones herpéticas tipo 1. Int. J. Odontostomat., 8(3):323-328, 2014. RESUMEN:El herpes simple tipo I es una patología infecciosa e inflamatoria de tipo vírico que se caracteriza por la aparición de lesiones ulcerativas extra orales, de duración aproximada de 14 días y que provocan gran picazón e incomodidad a las personas que lo padecen. El objetivo de este trabajo fue evaluar el uso la Melissa officinalis (toronjil) como terapia alternativa sobre el tiempo de remisión y el número de las lesiones extraorales en pacientes con herpes simple del comparado con el Aciclovir tópico al 5% como terapia convencional. Se realizó un ensayo clínico, aleatorizado y enmascarado simple, controlado. Se evaluó a 34 pacientes con lesiones herpéticas extra orales activas en la etapa primaria vesiculosa, sin patologías asociadas a alteraciones del sistema inmunológico como diabetes mellitus, alergias e hipertensión arterial. Se les administró el tratamiento tópico y se les evalúo diariamente las lesiones herpéticas. La Melissa officinalis posee un efecto antiviral sobre lesiones herpéticas tipo 1 al igual que el Aciclovir tópico al 5%, pero la Melissa officinalis es más efectivo en la disminución de días con lesiones herpéticas (valor p=0,05). La Melissa officinalis tiene un efecto antiviral positivo sobre lesiones herpéticas tipo 1, disminuyendo el número total de días con las lesiones herpéticas en comparación con el Aciclovir tópico al 5%.PALABRAS CLAVE: herpes simple tipo 1, patología viral, lesiones ulcerativas, Melissa officinalis, aciclovir tópico al 5%, antiviral.

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Local therapy of herpes simplex with dried extract from Melissa officinalis

Cited by 78 publications

References 6 publications

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

<i>Melissa officinalis</i> Extract Inhibits Attachment of Herpes Simplex Virus in vitro

Impact of Ethanolic Lamiaceae Extracts on Herpesvirus Infectivity in Cell Culture

Estudio Comparativo entre Terapia con Melissa officinalis vs. Tratamiento Convencional con Aciclovir Tópico al 5% para Lesiones Herpéticas Tipo 1

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