Local tissue reaction after the application of topical hemostatic agents in a rat partial nephrectomy model

Abstract

Various hemostatics are used for renal surgical procedures. We investigated the hemostatic efficacy of cellulose derivatives on the model of partial nephrectomy in rats focusing on the local reaction of renal parenchyma. A total of 50 Wistar rats were divided into five groups of 10 animals each. Partial nephrectomy of the caudal pole without hilar vascular control was performed. Oxidized cellulose (OC), sodium salt of oxycellulose (OCN), carboxymethyl cellulose (CMC), dialdehyde cellulose (DAC), and gelatin-based hemostatic (C) were applied to the bleeding wounds. The time to hemostasis was monitored. Half of the animals were euthanized after 3 days, the second half 30 days from the experiment start date. The left kidney was excised and subjected to histopathological examination. The biochemical data was subjected to statistical analysis. The time to hemostasis in all groups was significantly less than in the C group (in OC p = 0.0057, OCN p = 0.0039, CMC and DAC p = 0.0001). In the C group, massive hemorrhages and necrosis did occur. In the OC and OCN groups, there were regenerative changes, a receding inflammatory reaction and hemorrhage. DAC caused an immune reaction and massive interstitial hemorrhages with biochemical signs of liver damage. Parenchyma in CMC revealed a reduction of necrosis and interstitial hemorrhages with regenerative processes. The most effective hemostatics were CMC and OC, achieving the best results both in the time to hemostasis, and for histopathological evaluation.

Composite Hemostatic Nonwoven Textiles Based on Hyaluronic Acid, Cellulose, and Etamsylate

“…Preparations consisting of hyaluronic acid and cellulose and potentially with an active pharmaceutical ingredient for hemostatic application are unique in this category and cannot easily be compared with materials that have been used previously. Comparing the results of another cellulose hemostatic study utilizing the same in vivo model, it can be assumed that the new composite preparations proved to possess more powerful hemostatic properties [38]. The carboxymethyl cellulose textiles also showed significant effect in comparison with the oxycellulose version (HA/OX) of the new product (p < 0.01 for HA/CMC, p < 0.05 for HA/CMC/ET).…”

“…From the beginning, the Scientific Committee for the Protection of Animals at the university, along with the Ministry of Agriculture, approved the in vivo concept (approval number 19-2016) according to the submitted experimental protocols. A partial nephrectomy model in rats was then performed [38]. Forty male Wistar laboratory rats (AnLab, Prague, Czech Republic) with an average weight of 244 ± 27 g were randomly assigned to 4 test groups of 10 rats.…”

Hemostatic implant based on cellulose derivates

“…Результаты исследований по оценке гемостатической эффективности композиционного полимерного материала имеют схожие результаты с ранее представленными работами при сравнительном исследовании аппликационных гемостатических средств [14,15]. В одном из исследований при резекции левого каудального полюса почки у крыс в эксперименте с использованием «Gelitacel» и «Gelaspon», время остановки кровотечения составило 1,40±0,18 мин и 2,33±0,27 мин, что демонстрирует гемостатическую активность материалов на основе производных целлюлозы [16]. В нашем случае при использовании КПМ время гемостаза составило 34,0±2,5 сек.…”

Oxidised cellulose powder for haemostasis following sinus surgery: a pilot randomised trial

“…We found no evidence that oxidised cellulose powder causes more crusting or synechiae in the nose up to three months after surgery. Oxidised cellulose is known to induce minimal inflammatory reaction, 28 and is usually absorbed from the surgical site within a few days of application. In this study, oxidised cellulose powder was compared with a polyvinyl alcohol pack, which is also known to induce little inflammatory reaction.…”