Local Zinc Chloride Release From a Calcium Sulfate Carrier Enhances Fracture Healing

Krell, Ethan S.; Ippolito, Joseph A.; Montemurro, Nicholas J.; Lim, Phillip H; Vincent, Richard A; Hreha, Jeremy; Cottrell, Jessica; Sudah, Suleiman Y.; Muñoz, Maximilian F; Pacific, Kristen; Benevenia, Joseph; O’Connor, J. P.

doi:10.1097/bot.0000000000000748

Cited by 7 publications

(15 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…29 Krell et al ., using a calcium sulfate paste as a carrier, found that an intramedullary dose of 1 mg/kg ZnCl 2 (ZnCl 2 to body weight) significantly improved rat femur fracture healing as compared to a 3 mg/kg dose of ZnCl 2 . 15…”

Section: Discussionmentioning

confidence: 99%

“…In prior work, we demonstrated that zinc salts and other insulin-mimetic metal salts can promote bone regeneration in rats. 15,16 Therefore, we hypothesized that zinc could be a possible osteogenic adjuvant for bone allograft. 17 We tested this hypothesis by adding different zinc salts to commonly used DBM and cancellous bone chip allograft and evaluated the zinc-allograft for osteogenic activity in a rat femoral defect model.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improved osteogenesis in rat femur segmental defects treated with human allograft and zinc adjuvants

Kanjilal

Grieg

Culbertson

et al. 2021

Exp Biol Med (Maywood)

Self Cite

View full text Add to dashboard Cite

Bone allograft is widely used to treat large bone defects or complex fractures. However, processing methods can significantly compromise allograft osteogenic activity. Adjuvants that can restore the osteogenic activity of processed allograft should improve clinical outcomes. In this study, zinc was tested as an adjuvant to increase the osteogenic activity of human allograft in a Rag2 null rat femoral defect model. Femoral defects were treated with human demineralized bone matrix (DBM) mixed with carboxy methyl cellulose containing ZnCl2 (0, 75, 150, 300 µg) or Zn stearate (347 µg). Rat femur defects treated with DBM-ZnCl2 (75 µg) and DBM-Zn stearate (347 µg) showed increased calcified tissue in the defect site compared to DBM alone. Radiograph scoring and µCT (microcomputed tomography) analysis showed an increased amount of bone formation at the defects treated with DBM-Zn stearate. Use of zinc as an adjuvant was also tested using human cancellous bone chips. The bone chips were soaked in ZnCl2 solutions before being added to defect sites. Zn adsorbed onto the chips in a time- and concentration-dependent manner. Rat femur defects treated with Zn-bound bone chips had more new bone in the defects based on µCT and histomorphometric analyses. The results indicate that zinc supplementation of human bone allograft improves allograft osteogenic activity in the rat femur defect model.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved osteogenesis in rat femur segmental defects treated with human allograft and zinc adjuvants

Kanjilal

Grieg

Culbertson

et al. 2021

Exp Biol Med (Maywood)

Self Cite

View full text Add to dashboard Cite

show abstract

“…What is more, Zn shows antibacterial and anti-inflammatory properties and inhibits osteoclasts differentiation [ 40 ]. Other authors have reported a positive influence of Zn on bone fracture healing [ 41 ]. The incorporation of zinc in various calcium phosphate-based biomaterials for bone regeneration led to higher new bone formation and bone–implant contact in vivo [ 42 – 45 ].…”

Section: Discussionmentioning

confidence: 99%

Electrospun polycaprolactone membranes with Zn-doped bioglass for nasal tissues treatment

Rajzer

Dziadek

Kurowska

et al. 2019

J Mater Sci: Mater Med

View full text Add to dashboard Cite

In this work, composite membranes were investigated as future components of a layered implant for the reconstruction of nasal septum. Incorporation of zinc ions into nasal implants could potentially provide antibacterial properties to decrease or eliminate bacterial infections and subsequent surgical complications. Two types of membranes were prepared using an electrospinning method: PCL with bioglass and PCL with bioglass doped with Zn. The aim of this work was to investigate the influence of bioglass addition on the morphology, fiber diameter and composition of the membranes. The apatite-forming ability was examined in Simulated Body Fluid (SBF). The cytotoxicity of the membranes, ALP activity and in vitro mineralization were evaluated in cell culture. The mineralization and ALP activity was higher for polycaprolactone membranes modified with Zn doped bioglass than compared to pure PCL membranes or control material. The results proved that the presence of Zn 2+ in the electrospun membranes = influence the osteogenic differentiation of cells.

show abstract

“…Oral intake of Zn can increase serum Zn and alkaline phosphatase (ALP) activity and promote fracture healing in patients [ 200 ]. Local injection of ZnCl 2 promoted fracture healing of bones in rats, and increased the mechanical strength and stability after fracture healing [ 201 , 202 ].…”

Section: Mg-based Bms For Oral and Maxillofacial Applicationmentioning

confidence: 99%

“…Zn has a well-known insulin-mimetic property, which is conducive to cartilage regeneration [ 293 ]. Local application of ZnCl 2 enhanced cartilage formation and improved endochondral ossification [ 201 , 202 ]. In vitro experiments showed that a low concentration of Zn ions could increase the proliferation rate of growth plate chondrocytes by 40–50% and increased the synthesis of highly charged proteoglycan molecules, thus reducing mineralization [ 294 ].…”

Section: Perspectivesmentioning

confidence: 99%

Research status of biodegradable metals designed for oral and maxillofacial applications: A review

Xia

Yang

Zheng

et al. 2021

Bioactive Materials

View full text Add to dashboard Cite

The oral and maxillofacial regions have complex anatomical structures and different tissue types, which have vital health and aesthetic functions. Biodegradable metals (BMs) is a promising bioactive materials to treat oral and maxillofacial diseases. This review summarizes the research status and future research directions of BMs for oral and maxillofacial applications. Mg-based BMs and Zn-based BMs for bone fracture fixation systems, and guided bone regeneration (GBR) membranes, are discussed in detail. Zn-based BMs with a moderate degradation rate and superior mechanical properties for GBR membranes show great potential for clinical translation. Fe-based BMs have a relatively low degradation rate and insoluble degradation products, which greatly limit their application and clinical translation. Furthermore, we proposed potential future research directions for BMs in the oral and maxillofacial regions, including 3D printed BM bone scaffolds, surface modification for BMs GBR membranes, and BMs containing hydrogels for cartilage regeneration, soft tissue regeneration, and nerve regeneration. Taken together, the progress made in the development of BMs in oral and maxillofacial regions has laid a foundation for further clinical translation.

show abstract

Local Zinc Chloride Release From a Calcium Sulfate Carrier Enhances Fracture Healing

Abstract: The effective dose of ZnCl2 augmentation for the enhancement of fracture healing in rats was reduced 3-fold in this study compared with previous findings. Furthermore, CaSO4 acted synergistically with ZnCl2 to increase the mechanical strength and stability at the fracture site.

Cited by 7 publications

References 32 publications

Improved osteogenesis in rat femur segmental defects treated with human allograft and zinc adjuvants

Improved osteogenesis in rat femur segmental defects treated with human allograft and zinc adjuvants

Electrospun polycaprolactone membranes with Zn-doped bioglass for nasal tissues treatment

Research status of biodegradable metals designed for oral and maxillofacial applications: A review

Contact Info

Product

Resources

About