Localisation of ABCA1 in First Trimester and Term Placental Tissues

Albrecht, Christiane; Nikitina, Liudmila; Wenger, F.; Baumann, Marc; Surbek, Daniel; Körner, Meike

doi:10.1016/j.placenta.2010.05.010

Cited by 16 publications

(11 citation statements)

References 4 publications

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“…Classically, it has been described that the efflux to HDL is mediated by ABCG1 and to ApoA-I by ABCA1 19,20,[48][49][50] . Our results of in situ localization are in agreement with previous papers describing ABCA1 in the syncytiotrophoblast layer of the placenta 20,49,[51][52][53] . Although a proper marker for syncytiotrophoblast apical and basal membrane is required in the same tissue section, our findings are also in accordance with these reports and suggests a distribution of the transporter on the apical and basal sides of the trophoblast layer, indicating a possible role in the export of cholesterol to the maternal and also the foetal circulation.…”

Section: Sr-bi and Hdl Uptakesupporting

confidence: 93%

Cholesterol uptake and efflux are impaired in human trophoblast cells from pregnancies with maternal supraphysiological hypercholesterolemia

Fuenzalida

Cantin

Kallol

et al. 2020

Sci Rep

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Maternal physiological (MpH) or supraphysiological hypercholesterolaemia (MSpH) occurs during pregnancy. Cholesterol trafficking from maternal to foetal circulation requires the uptake of maternal LDL and HDL by syncytiotrophoblast and cholesterol efflux from this multinucleated tissue to ApoA-I and HDL. We aimed to determine the effects of MSPH on placental cholesterol trafficking. Placental tissue and primary human trophoblast (pHt) were isolated from pregnant women with total cholesterol <280 md/dL (MPH, n = 27) or ≥280 md/dL (MSPH, n = 28). The lipid profile in umbilical cord blood from MpH and MSpH neonates was similar. the abundance of LDL receptor (LDLR) and HDL receptor (SR-Bi) was comparable between MSpH and MpH placentas. However, LDLR was localized mainly in the syncytiotrophoblast surface and was associated with reduced placental levels of its ligand ApoB. in pHt from MSpH, the uptake of LDL and HDL was lower compared to MpH, without changes in LDLR and reduced levels of SR-BI. Regarding cholesterol efflux, in MSPH placentas, the abundance of cholesterol transporter ABCA1 was increased, while ABCG1 and SR-BI were reduced. In PHT from MSPH, the cholesterol efflux to ApoA-I was increased and to HDL was reduced, along with reduced levels of ABCG1, compared to MPH. Inhibition of SR-BI did not change cholesterol efflux in PHT. The TC content in PHT was comparable in MpH and MSpH cells. However, free cholesterol was increased in MSpH cells. We conclude that MSPH alters the trafficking and content of cholesterol in placental trophoblasts, which could be associated with changes in the placenta-mediated maternal-to-foetal cholesterol trafficking. During the progress of human pregnancy, the concentrations of total cholesterol (TC) and low-(LDL) rises in a physiological (maternal physiological hypercholesterolemia, MPH) 1,2 or supraphysiological (maternal supraphysiological hypercholesterolemia, MSPH) 3,4 way. MSPH is determined in women with TC levels above a cutoff value of 280-300 mg/dL at term of pregnancy or above the 75th percentile for the different trimesters of pregnancy 1-6. Endothelial dysfunction of the macro-and the microvascular vessels of the placenta 4,7-9 as well as development of atherosclerosis in the foetal aorta 1,2,6 has been described in pregnancies with MSPH and increased LDL levels. This information suggest that MSPH could be related to the development of cardiovascular disease in the offspring later in life 2,6,10,11. Despite the increased maternal TC and LDL concentrations, the levels of TC and triglycerides (Tg) of neonates from MSPH pregnancies are comparable to those from MPH pregnancies, suggesting a possible regulation of placental maternal-to-foetal cholesterol trafficking across the placenta 7,12 .

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Section: Sr-bi and Hdl Uptakesupporting

confidence: 93%

Cholesterol uptake and efflux are impaired in human trophoblast cells from pregnancies with maternal supraphysiological hypercholesterolemia

Fuenzalida

Cantin

Kallol

et al. 2020

Sci Rep

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“…ABCA1 is predominantly localized on the basolateral side of the cytotrophoblast layer of the first trimester of pregnancy, suggesting its potential role in the efflux of cholesterol to the fetal side (29). While findings by Albrecht et al were similar to that by Bhattacharjee et al , Albrecht et al also found localization of ABCA1 in early pregnancy in the possible Hofbauer cells in the villous stroma (30). Furthermore, while Albrecht et al found variable but confirmed staining of ABCA1 in the cytoplasm of villous syncytiotrophoblasts by immunohistochemistry (30), Bhattacharjee et al had shown ABCA1 staining by immunofluorescence only on the apical side of a few villous, syncytiotrophoblasts (29).…”

Section: Abc Transporters In the Placentasupporting

confidence: 68%

“…While findings by Albrecht et al were similar to that by Bhattacharjee et al , Albrecht et al also found localization of ABCA1 in early pregnancy in the possible Hofbauer cells in the villous stroma (30). Furthermore, while Albrecht et al found variable but confirmed staining of ABCA1 in the cytoplasm of villous syncytiotrophoblasts by immunohistochemistry (30), Bhattacharjee et al had shown ABCA1 staining by immunofluorescence only on the apical side of a few villous, syncytiotrophoblasts (29). Baumann et al showed that the ABCA1 mRNA expression is dependent on gestational age and displayed significantly higher ABCA1 mRNA levels in preterm compared to term placenta.…”

Section: Abc Transporters In the Placentasupporting

confidence: 68%

Placental ABC Transporters: Biological Impact and Pharmaceutical Significance

et al. 2016

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The human placenta fulfills a variety of essential functions during prenatal life. Several ABC transporters are expressed in the human placenta, where they play a role in the transport of endogenous compounds and may protect the fetus from exogenous compounds such as therapeutic agents, drugs of abuse, and other xenobiotics. To date, considerable progress has been made toward understanding ABC transporters in the placenta. Recent studies on the expression and functional activities are discussed. This review discusses the placental expression and functional roles of several members of ABC transporter subfamilies B, C, and G including MDR1/P-glycoprotein, the MRPs, and BCRP, respectively. Since placental ABC transporters modulate fetal exposure to various compounds, an understanding of their functional and regulatory mechanisms will lead to more optimal medication use when necessary in pregnancy.

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“…ABCA1 is highly expressed in the placenta [46–48] and was suggested to play a role in cholesterol transfer from the maternal to the fetal circulation [49]. Moreover, previous studies from our laboratories demonstrated a reduced ABCA1 protein expression in the syncytiotrophoblast of pre-eclamptic placental tissues by immunohistochemistry [50], and decreased placental mRNA expression in pre-eclampsia as compared to controls (unpublished data).…”

Section: Resultsmentioning

confidence: 88%

Increased Placental Phospholipid Levels in Pre-Eclamptic Pregnancies

Huang

Jain

Baumann

et al. 2013

IJMS

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Physiological pregnancy is associated with an increase in lipids from the first to the third trimester. This is a highly regulated response to satisfy energy and membrane demands of the developing fetus. Pregnancy disorders, such as pre-eclampsia, are associated with a dysregulation of lipid metabolism manifesting in increased maternal plasma lipid levels. In fetal placental tissue, only scarce information on the lipid profile is available, and data for gestational diseases are lacking. In the present study, we investigated the placental lipid content in control versus pre-eclamptic samples, with the focus on tissue phospholipid levels and composition. We found an increase in total phospholipid content as well as changes in individual phospholipid classes in pre-eclamptic placental tissues compared to controls. These alterations could be a source of placental pathological changes in pre-eclampsia, such as lipid peroxide insult or dysregulation of lipid transport across the syncytiotrophoblast.

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Localisation of ABCA1 in First Trimester and Term Placental Tissues

Cited by 16 publications

References 4 publications

Cholesterol uptake and efflux are impaired in human trophoblast cells from pregnancies with maternal supraphysiological hypercholesterolemia

Cholesterol uptake and efflux are impaired in human trophoblast cells from pregnancies with maternal supraphysiological hypercholesterolemia

Placental ABC Transporters: Biological Impact and Pharmaceutical Significance

Increased Placental Phospholipid Levels in Pre-Eclamptic Pregnancies

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