Localisation of Eight Additional Genes in the Human Major Histocompatibility Complex, Including the Gene Encoding the Casein Kinase II Beta Subunit, and DNA Sequence Analysis of the Class III Region

Mr, Albertella; Jones, H.; Thomson, Wendy; Mg, Olavesen; Neville, Matt J.; Campbell, R. Duncan

doi:10.3109/10425179609015638

Cited by 5 publications

(2 citation statements)

References 4 publications

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“…The α-subunits are encoded by two distinct homologous genes, CSNK2A1, which encodes CK2α [4], and CSNK2A2, which encodes CK2α’ [5]. The β-subunit is encoded by CSNK2B [6]. CK2β is not only a simple on–off regulator of the catalytic activity of CK2α but also regulates thermostability, substrate specificity, and the ability to attach and penetrate cell membranes [7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Protein Kinase CK2—A Putative Target for the Therapy of Diabetes Mellitus?

Ampofo

Nalbach

Menger

et al. 2019

IJMS

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Since diabetes is a global epidemic, the development of novel therapeutic strategies for the treatment of this disease is of major clinical interest. Diabetes is differentiated in two types: type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM). T1DM arises from an autoimmune destruction of insulin-producing β-cells whereas T2DM is characterized by an insulin resistance, an impaired insulin reaction of the target cells, and/or dysregulated insulin secretion. In the past, a growing number of studies have reported on the important role of the protein kinase CK2 in the regulation of the survival and endocrine function of pancreatic β-cells. In fact, inhibition of CK2 is capable of reducing cytokine-induced loss of β-cells and increases insulin expression as well as secretion by various pathways that are regulated by reversible phosphorylation of proteins. Moreover, CK2 inhibition modulates pathways that are involved in the development of diabetes and prevents signal transduction, leading to late complications such as diabetic retinopathy. Hence, targeting CK2 may represent a novel therapeutic strategy for the treatment of diabetes.

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Section: Introductionmentioning

confidence: 99%

Protein Kinase CK2—A Putative Target for the Therapy of Diabetes Mellitus?

Ampofo

Nalbach

Menger

et al. 2019

IJMS

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“…Other less abundant transcripts were also detected (see Figure 6A ) which were confirmed when primers from exons 5 or 6 of CSNK2B were used in the PCR reactions (Figures 6C and 6D ). Amplification under the same conditions of the CSNK2B gene using primers from exons 2 and 7 resulted in the appearance of a single band of 645 bp (Figure 6E ) corresponding to only one RNA form, the one described in the literature [ 29 ].…”

Section: Resultsmentioning

confidence: 89%

Regulation of expression of two LY-6 family genes by intron retention and transcription induced chimerism

et al. 2008

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Background: Regulation of the expression of particular genes can rely on mechanisms that are different from classical transcriptional and translational control. The LY6G5B and LY6G6D genes encode LY-6 domain proteins, whose expression seems to be regulated in an original fashion, consisting of an intron retention event which generates, through an early premature stop codon, a non-coding transcript, preventing expression in most cell lines and tissues.

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Selection of novel reference genes for use in the human central nervous system: a BrainNet Europe Study

Durrenberger¹,

Fernando²,

Magliozzi

et al. 2012

Acta Neuropathol

100

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The use of an appropriate reference gene to ensure accurate normalisation is crucial for the correct quantification of gene expression using qPCR assays and RNA arrays. The main criterion for a gene to qualify as a reference gene is a stable expression across various cell types and experimental settings. Several reference genes are commonly in use but more and more evidence reveals variations in their expression due to the presence of on-going neuropathological disease processes, raising doubts concerning their use. We conducted an analysis of genome-wide changes of gene expression in the human central nervous system (CNS) covering several neurological disorders and regions, including the spinal cord, and were able to identify a number of novel stable reference genes. We tested the stability of expression of eight novel (ATP5E, AARS, GAPVD1, CSNK2B, XPNPEP1, OSBP, NAT5 and DCTN2) and four more commonly used (BECN1, GAPDH, QARS and TUBB) reference genes in a smaller cohort using RT-qPCR. The most stable genes out of the 12 reference genes were tested as normaliser to validate increased levels of a target gene in CNS disease. We found that in human post-mortem tissue the novel reference genes, XPNPEP1 and AARS, were efficient in replicating microarray target gene expression levels and that XPNPEP1 was more efficient as a normaliser than BECN1, which has been shown to change in expression as a consequence of neuronal cell loss. We provide herein one more suitable novel reference gene, XPNPEP1, with no current neuroinflammatory or neurodegenerative associations that can be used for gene quantitative gene expression studies with human CNS post-mortem tissue and also suggest a list of potential other candidates. These data also emphasise the importance of organ/tissue-specific stably expressed genes as reference genes for RNA studies.

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Localisation of Eight Additional Genes in the Human Major Histocompatibility Complex, Including the Gene Encoding the Casein Kinase II Beta Subunit, and DNA Sequence Analysis of the Class III Region

Cited by 5 publications

References 4 publications

Protein Kinase CK2—A Putative Target for the Therapy of Diabetes Mellitus?

Protein Kinase CK2—A Putative Target for the Therapy of Diabetes Mellitus?

Regulation of expression of two LY-6 family genes by intron retention and transcription induced chimerism

Selection of novel reference genes for use in the human central nervous system: a BrainNet Europe Study

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